System, method, and product for scanning of biological materials
First Claim
1. ) A scanning system, comprising:
- one or more optical elements constructed and arranged to direct a excitation beam at a probe array;
one or more detectors constructed and arranged to receive reflected intensity data responsive to the excitation beam, wherein the reflected intensity data is responsive, at least in part, to a focusing distance between an optical element and the probe array;
a transport frame constructed and arranged to adjust the focusing distance in a first direction with respect to the probe array;
an auto-focuser constructed and arranged to determine a best plane of focus based, at least in part, upon one or more characteristics of the reflected intensity data as received at two or more focusing distances;
and wherein;
the one or more detectors are further constructed and arranged to receive a plurality of pixel intensity values based, at least in part, upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus; and
the system further comprises an image generator constructed and arranged to associate each of the pixel intensity values with one or more image pixel positions of a probe array based, at least in part, upon one or more position correction values.
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Accused Products
Abstract
An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.
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Citations
107 Claims
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1. ) A scanning system, comprising:
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one or more optical elements constructed and arranged to direct a excitation beam at a probe array;
one or more detectors constructed and arranged to receive reflected intensity data responsive to the excitation beam, wherein the reflected intensity data is responsive, at least in part, to a focusing distance between an optical element and the probe array;
a transport frame constructed and arranged to adjust the focusing distance in a first direction with respect to the probe array;
an auto-focuser constructed and arranged to determine a best plane of focus based, at least in part, upon one or more characteristics of the reflected intensity data as received at two or more focusing distances;
and wherein;
the one or more detectors are further constructed and arranged to receive a plurality of pixel intensity values based, at least in part, upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus; and
the system further comprises an image generator constructed and arranged to associate each of the pixel intensity values with one or more image pixel positions of a probe array based, at least in part, upon one or more position correction values.
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2. ) A method, comprising the acts of:
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receiving a plurality of pixel intensity values based, at least in part, upon detected emissions from a plurality of probe features disposed on a probe array; and
associating each of the pixel intensity values with one or more image pixel positions of a probe array image based, at least in part, upon one or more position correction values. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. ) A scanning system, comprising:
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one or more detectors constructed and arranged to receive a plurality of pixel intensity values based, at least in part, upon detected emissions from a plurality of probe features disposed on a probe array; and
a corrected image generator constructed and arranged to associate each of the pixel intensity values with one or more image pixel positions of a probe array image based, at least in part, upon one or more position correction values - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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57. ) A method, comprising the acts of,
directing an excitation beam at a probe array; -
receiving reflected intensity data responsive to the excitation beam, wherein the intensity data is responsive, at least in part, to a focusing distance between an optical element and the probe array;
adjusting the focusing distance in a first direction with respect to the probe array; and
repeating the steps of receiving and adjusting for a number of iterations;
determining a best plane of focus based, at least in part, upon one or more characteristics of the reflected intensity data at the adjusted focusing distances. - View Dependent Claims (58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68)
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69. ) A scanning system, comprising,
one or more optical elements constructed and arranged to direct an excitation beam at a probe array; -
one or more detectors constructed and arranged to receive reflected intensity data responsive to the excitation beam, wherein the intensity data is determined, at least in part, by a focusing distance between an optical element and the probe array;
an auto-focuser constructed and arranged to determine a best plane of focus based, at least in part, upon one or more characteristics of the reflected intensity data as received at two or more focusing distances. - View Dependent Claims (70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80)
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81. ) A method, comprising the acts of:
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storing one or more probe arrays in a magazine, wherein each of the plurality of probe arrays is housed in a probe array cartridge;
reversibly transporting a first probe array cartridge between the magazine and a scanning system; and
advancing the magazine by one or more positions to a second probe array cartridge. - View Dependent Claims (82, 83, 84, 85, 86, 87, 88, 89, 90, 91)
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92. ) A scanning system, comprising:
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a cooled storage chamber constructed and arranged to store one or more probe arrays in a magazine, wherein each of the plurality of probe arrays is housed in a probe array cartridge;
a cartridge transport assembly constructed and arranged to reversibly transport a first probe array cartridge between the magazine and a scanning system; and
a linear motor constructed and arranged to advance the magazine by one or more positions to a second probe array cartridge. - View Dependent Claims (93, 94, 95, 96, 97, 98, 99, 100, 101, 102)
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103. ) A scanning system, comprising:
a service application constructed and arranged to perform one or more calibration methods, wherein the service application includes an interface to a plurality of elements of the scanning system. - View Dependent Claims (104, 105, 106, 107)
Specification