Oligonucleotide-facilitated coalescence
First Claim
Patent Images
1. A method for facilitating the coalescence of two members selected from the group consisting of cells, vesicles, liposomes and lipidic particles comprising:
- a. incorporating in the first of said members a first outwardly projecting anchored oligonucleotide containing a first terminal hydrophobic anchoring moiety, b. incorporating in the second of said members a second outwardly projecting anchored oligonucleotide complementary to said first anchored oligonucleotide and also containing a second terminal hydrophobic anchoring moiety, and c. incubating said first and second members under conditions promoting hybridization of said first and second outwardly projecting anchored oligonucleotides.
3 Assignments
0 Petitions
Accused Products
Abstract
Coalescence of cells or other membrane-bound entities is facilitated by anchoring an outwardly projecting first oligonucleotide in one member and an outwardly projecting second oligonucleotide, complementary to the first, in a second member and incubating under hybridizing conditions. Liposomes may be coalesced with cells to deliver hydrophilic agents thereto, such as DNA probes or drugs. Kits containing complementary oligonucleotides containing hydrophobic anchoring moieties may be used.
-
Citations
12 Claims
-
1. A method for facilitating the coalescence of two members selected from the group consisting of cells, vesicles, liposomes and lipidic particles comprising:
-
a. incorporating in the first of said members a first outwardly projecting anchored oligonucleotide containing a first terminal hydrophobic anchoring moiety, b. incorporating in the second of said members a second outwardly projecting anchored oligonucleotide complementary to said first anchored oligonucleotide and also containing a second terminal hydrophobic anchoring moiety, and c. incubating said first and second members under conditions promoting hybridization of said first and second outwardly projecting anchored oligonucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
- 8. A liposome or lipidic particle containing an entrapped hydrophilic substance and having incorporated therein an outwardly projecting anchored oligonucleotide containing a terminal hydrophobic anchoring moiety.
- 11. A kit of reagents comprising a pair of separately packaged complementary oligonucleotides each containing a terminal hydrophobic anchoring moiety.
Specification