Process for regulating gene expression

• US 20040014052A1
• Filed: 07/22/2002
• Published: 01/22/2004
• Est. Priority Date: 07/22/2002
• Status: Abandoned Application

• Current Assignee: Biomed Solutions, LLC
• Original Assignee: Biomed Solutions, LLC
• Inventors: Kurtz, Warren H., MacDonald, Stuart G., Greenwald, Howard J.
• Application Number:
  US10/200,980
• Publication Number:
  US 20040014052A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07K 14/4702 Regulators; Modulating activity
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 15/85 for animal cells

Abstract

A process for stimulating the expression of a gene in which two different DNA constructs are disposed within a biological system and contacted with electromagnetic radiation, thereby causing each of the DNA constructs to express a gene product.

Claims

1. A process for stimulating the expression of a gene comprising the steps of disposing a first electromagnetic responsive DNA construct in a biological system, disposing a second electromagnetic responsive DNA construct in such biological system, stimulating said first electromagnetic responsive DNA construct with electromagnetic energy, stimulating said second electromagnetic responsive DNA construct with electromagnetic energy, expressing a first RNA transcript from said first electromagnetic responsive DNA construct, and expressing a second RNA transcript from said second electromagnetic responsive DNA construct.

2. A biological system comprised of a first electromagnetic responsive gene product, a second electromagnetic responsive gene product, means for stimulating said first electromagnetic responsive gene product with a electromagnetic energy, means for stimulating said second electromagnetic responsive gene product with electromagnetic energy, means for expressing a first RNA transcript with said first electromagnetic responsive gene product, and means for expressing a second RNA transcript with said second electromagnetic responsive gene product.

3. A bioreactor comprised of a biological system, wherein said biological system is comprised of a first electromagnetic responsive DNA construct comprised of a electromagnetic response element ligated to a first polynucleotide sequence, means for stimulating said first electromagnetic response DNA construct with electromagnetic energy, and means for expressing a first RNA transcript from said first electromagnetic response DNA constructt, wherein said bioreactor is comprised of a sensor for sensing a property of said biological system and means for generating an electromagnetic signal in response to information received from said sensor.

4. The bioreactor as recited in claim 3, wherein said bioreactor is comprised of a first electromagnetic coil assembly.

5. The bioreactor as recited in claim 4, wherein said bioreactor is comprised of a controller operatively connected to said first electromagnetic coil assembly.

6. The bioreactor as recited in claim 5, wherein said sensor is operatively connected to said controller.

7. The bioreactor as recited in claim 6, wherein said bioreactor is comprised of a power supply connected to said first electromagnetic coil assembly.

8. The bioreactor as recited in claim 7, wherein said bioreactor further comprises a exit line.

9. The bioreactor as recited in claim 8, wherein said electromagnetic coil is disposed around said exit line.

10. The bioreactor as recited in claim 7, further comprising a reactor vessel comprised of hollow fibers.

11. The bioreactor as recited in claim 10, wherein said hollow fibers are disposed within a hollow fiber bioreactor comprised of a multiplicity said hollow fibers on whose outer surface a bonded a multiplicity of cells.

12. The bioreactor as recited in claim 11, wherein said electromagnetic coil assembly is disposed around said hollow fiber bioreactor.

13. The bioreactor as recited in claim 12, further a second electromagnetic coil assembly disposed around a multiplicity of hollow fibers.

14. The bioreactor as recited in claim 13, wherein said second electromagnetic coil assembly is comprised of a continuous electromagnetic coil.

15. The bioreactor as recited in claim 13, wherein said second electromagnetic coil assembly is comprised of a multiplicity of individual closed coils that are adjacent to each other but not contiguous with each other.

16. The bioreactor as recited in claim 12, wherein a multiplicity of magnetic particles are disposed on said hollow fibers.

17. The bioreactor as recited in claim 16, wherein a multiplicity of cells are disposed on said hollow fibers.

18. The bioreactor as recited in claim 11, further comprising means for delivering a first electromagnetic signal to a first region of said hollow fiber bioreactor.

19. The bioreactor as recited in claim 18, further comprising means for delivering a second electromagnetic signal to a second region of said hollow fiber bioreactor.

20. The bioreactor as recited in claim 19, further comprising means for delivering a third electromagnetic signal to a third region of said hollow fiber bioreactor.

21. The bioreactor as recited in claim 7, wherein said bioreactor is comprised of means for circulating a cell culture within said bioreactor.

22. The bioreactor as recited in claim 21, wherein said means for circulating said cell culture is comprised of a pump.

22-1. The bioreactor as recited in claim 21, wherein said pump is a variable pump.

23. The bioreactor as recited in claim 22, wherein said bioreactor is comprised of a mean a in-line mixer.

24. The bioreactor as recited in claim 23, wherein said bioreactor is comprised of a multiplicity of said in-line mixers.

25. The bioreactor as recited in claim 24, wherein said bioreactor is comprised of a multiplicity of sensors.

26. The bioreactor as recited in claim 25, wherein said bioreactor is comprised of a multiplicity of input ports.

27. The bioreactor as recited in claim 7, further comprising a second electromagnetic coil assembly connected to said power supply.

28. The bioreactor as recited in claim 7, further comprising a third electromagnetic coil assembly connected to said power supply.

29. The bioreactor as recited in claim 28, wherein said bioreactor is comprised of a closed loop.

30. The bioreactor as recited in claim 29, wherein said first electromagnetic coil is disposed around a first section of said closed loop.

31. The bioreactor as recited in claim 30, wherein said second electromagnetic coil is disposed around a second section of said closed loop.

32. The bioreactor as recited in claim 31, wherein said third electromagnetic coil is disposed around said third section of said closed loop.

33. The bioreactor as recited in claim 7, wherein said bioreactor is comprised of a closed loop.

34. The bioreactor as recited in claim 33, wherein said closed loop is comprised of conductive fluid.

35. The bioreactor as recited in claim 34, wherein said conductive fluid has a conductivity of from about 0.5 to about 100 milliSiemens.

36. The bioreactor as recited in claim 35, wherein said conductive fluid has a conductivity of from about 5 to about 30 milliSiemens.

37. The bioreactor as recited in claim 36, further comprising means for circulating said conductive fluid within said closed loop.

38. The bioreactor as recited in claim 39, wherein said closed loop is disposed around a transformer core.

39. The bioreactor as recited in claim 38, wherein said transformer core is a torroidal transformer core.

40. The bioreactor as recited in claim 39, wherein said first electromagnetic oil is disposed around said torroidal transformer core.

41. The bioreactor as recited in claim 40, further comprising means for sensing a property of said conductive fluid.

42. The bioreactor as recited in claim 42, further comprising a controller connected to said means for sensing a property of said conductive fluid.

43. The bioreactor as recited in claim 7, wherein said bioreactor is comprised of a biological system comprising an electromagnetic response element linked to a first gene that expresses a product, an electromagnetic response element linked to a second gene that expresses a regulatory control factor, and an electromagnetic response element linked to a third gene that expresses an anti-apototic

44. A biological system comprised of a first DNA construct comprised of a first electromagnetic response element ligated to a first polynucleotide sequence and a second DNA construct comprised of a second electromagnetic response element ligated to a second polynucleotide sequence, wherein said first DNA construct expresses a positive regulatory control element in response to exposure to electromagnetic radiation, and wherein said second DNA construct expresses a negative regulatory control element in response to electromagnetic radiation.

45. The biological system as recited in claim 44, wherein said biological system is comprised of implantable means for emitting said electromagnetic radiation.

46. The biological system as recited in claim 45, wherein said biological system is comprised of an implantable sensor.

47. The biological system as recited in claim 46, wherein said biological system is comprised of an implantable pump.

48. A process for regulating a biological system, comprising the steps of:

(a) disposing within said biological system a first electromagnetic response element ligated to a first polynucleotide sequence and a second DNA construct comprised of a second electromagnetic response element ligated to a second polynucleotide sequence, wherein said first DNA construct expresses a positive regulatory control element in response to exposure to electromagnetic radiation, and wherein said second DNA construct expresses a negative regulatory control element in response to electromagnetic radiation,

(b) exposing said first DNA construct to electromagnetic radiation, and

(c) exposing said second DNA construct to electromagnetic radiation.

49. The process as recited in claim 48, wherein said biological system is exposed to electromagnetic noise.

50. A biological system comprised of a first DNA construct comprised of a first electromagnetic response element ligated to a first polynucleotide sequence, wherein said first DNA construct expresses a negative regulatory control element in response to exposure to electromagnetic radiation.