Biosensor chip

• US 20040014054A1
• Filed: 01/16/2003
• Published: 01/22/2004
• Est. Priority Date: 03/30/2000
• Status: Abandoned Application

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Thewes, Roland, Frey, Alexander
• Application Number:
  US10/239,622
• Publication Number:
  US 20040014054A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The invention relates to a biosensor chip that is provided with a first electrode and a second electrode. The first electrode is provided with a holding area for holding probe molecules which can bind macromolecular biopolymers. The invention also relates to an integrated electric differentiating circuit by means of which an electric current can be detected and can be differentiated according to time, whereby said current is generated during a reduction/oxidation recycling procedure.

Claims

1. A biosensor chip, comprising:

a first electrode, which has a holding region for holding probe molecules which can bind macromolecular biopolymers;

a second electrode, the first electrode and the second electrode being designed in such a manner that a reduction/oxidation recycling operation can take place at these electrodes; and

an integrated electrical differentiator circuit, by means of which an electric current generated during the reduction/oxidation recycling operation can be recorded and can be differentiated over the course of time.

2. The biosensor chip as claimed in claim 1, further comprising:

a third electrode, the second electrode and the third electrode being designed in such a manner that the reduction/oxidation process takes place as part of the reduction/oxidation recycling operation at the second electrode and at the third electrode.

3. The biosensor chip as claimed in claim 2,

in which the first electrode has a first electrical potential;

in which the second electrode has a second electrical potential; and

in which the third electrode has a third electrical potential, the third electrical potential being selected in such a manner that during the reduction/oxidation recycling operation the reduction or oxidation takes place only at the second electrode and at the third electrode.

4. The biosensor chip as claimed in claim 3,

in which the third electrical potential is greater than the first electrical potential, and

in which the first electrical potential is greater than the second electrical potential.

5. The biosensor chip as claimed in claim 1 in which the holding region of the first electrode is coated with a material which can immobilize probe molecules.

6. The biosensor chip as claimed in claim 1 in which the holding region of the first electrode is designed to hold ligands, to which peptides or proteins can be bound.

7. The biosensor chip as claimed in claim 1 in which the holding region of the first electrode is designed to hold DNA probe molecules, to which DNA molecules can be bound.

8. The biosensor chip as claimed in claim 1 in which the holding region includes at least one of the following materials selected from the group consisting of hydroxyl radicals, epoxy radicals, amine radicals, acetoxy radicals, isocyanate radicals, succinimidyl ester radicals, thiol radicals, gold, silver, platinum, and titanium.

9. The biosensor chip as claimed in claim 1 in which the electrodes are arranged in an interdigitated electrode arrangement, the third electrode in each case being arranged between the first electrode and the second electrode.

10. The biosensor chip as claimed in claim 1 in which the first electrode and the second electrode and/or the third electrode are arranged in such a manner relative to one another that substantially uncurved field lines of an electric field, which is produced between the first electrode and the second electrode and/or the third electrode, can form between the first electrode and the second electrode and/or the third electrode.

11. The biosensor chip as claimed in claim 1 in which the differentiator circuit is electrically coupled to the second electrode.

12. The biosensor chip as claimed in claim 11, in which the differentiator circuit is electrically coupled to the second electrode via a current-voltage converter.

13. The biosensor chip as claimed in claim 1 having a reference circuit which has the same structure as the differentiator circuit and which can be used to generate an electrical reference signal.

14. The biosensor chip as claimed in claim 13, having an evaluation unit for evaluating the electrical signals generated by the differentiator circuit and by the reference circuit, it being possible to determine the increase in the curve of the electrical current generated during the reduction/oxidation recycling operation as a function of time using the evaluation unit.

15. The biosensor chip as claimed in claim 1 further comprising:

a multiplicity of first electrodes, which have a holding region for holding probe molecules which can bind macromolecular biopolymers; and

a multiplicity of second electrodes, the first and second electrodes being arranged in an electrode array.

16. The biosensor chip as claimed in claim 15, further comprising:

a multiplicity of third electrodes, the second electrodes and the third electrodes being designed in such a manner that the reduction/oxidation process takes place as part of the reduction/oxidation recycling operation at the second electrodes and at the third electrodes.