Capsules encapsulating solid particles of signal-generating organic substances and their use in vitro bioassays for detection of target molecules in a sample
First Claim
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1. Capsules encapsulating solid particles of signal-generating organic substances and carrying on the outer surface affinity molecules for specific recognition of and binding to target molecules.
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Abstract
The present invention refers to capsules encapsulating solid particles of signal-generating organic substances and carrying on the outer surface affinity molecules for specific recognition of and binding to target molecules in a sample. The invention is directed to the use of these capsules for signal production in the optical, electrochemical or chemical detection of target molecules. To obtain a signal the signal-generating organic substances are released and dissolved. A detection method and a kit are provided.
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29 Claims
- 1. Capsules encapsulating solid particles of signal-generating organic substances and carrying on the outer surface affinity molecules for specific recognition of and binding to target molecules.
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20. A method for optical, electrochemical or chemical detection of one or more target molecules in a sample using affinity-based interactions between the target molecule and an affinity molecule for specific recognition of the target molecule comprising the steps
i) incubating the target molecules with capsules encapsulating solid particles of signal-generating organic substances and carrying on their outer surface the corresponding affinity molecules, ii) separating the resulting target-affinity molecule complex from the capsules with unreacted affinity molecules on their surface, iii) releasing and dissolving the encapsulated solid particles of the signal-generating organic substances by treating the obtained target-affinity complex with physical or chemical means, iv) detecting or quantifying the signal which is generated by the released and dissolved signal-generating substances and which is directly or indirectly related to the amount of the target molecules.
- 24. Kit for optical, electrochemical or chemical detection of target molecules in a sample comprising capsules encapsulating solid particles of signal-generating organic substances and carrying on the outer surface affinity molecules for specific recognition and binding to a target molecule.
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26. Kit for optical, electrochemical or chemical detection of target molecules in a sample comprising
a) capsules encapsulating solid particles of signal-generating organic substances being designated to bind affinity molecules b) agents for the modification of affinity molecules to make them suitable to bind to the surface of the capsules c) agents for performing the binding reaction between the capsules and the affinity molecules.
Specification