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Method for examining reactivity and method for detecting a complex

  • US 20040014124A1
  • Filed: 06/30/2003
  • Published: 01/22/2004
  • Est. Priority Date: 08/31/2000
  • Status: Abandoned Application
First Claim
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45. A method of detecting a complex formed between an oligonucleotide of which base sequence is known and a component having a capability of binding to said oligonucleotide, comprising the steps of:

  • preparing at least one oligonucleotide of which base sequence is known;

    preparing at least two liquid test samples potentially containing a component having a capability of binding to said oligonucleotide;

    binding said oligonucleotide as a probe to a predetermined region on a solid substrate to produce a detection substrate;

    arranging a plurality of spots of said test samples at a predetermined amount to form an array of said test samples within said region with said oligonucleotide bound thereto;

    detecting whether a complex between said oligonucleotide and said component is present or not for each of said plurality of spots; and

    determining whether or not said component is contained in each of said liquid test samples, or how strong its binding capability to said oligonucleotide is, based on said detection.

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