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Preparation and xenotransplantation or porcine islets

  • US 20040014212A1
  • Filed: 06/25/2003
  • Published: 01/22/2004
  • Est. Priority Date: 10/17/2000
  • Status: Abandoned Application
First Claim
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1. A method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, the method including or comprising:

  • (i) harvesting the pancreas of piglets at or near full term gestation, and (ii) extracting islets from a culture of the harvested pancreas using a suitable collagenase, the culture of the harvested pancreas being—

    (a) of mechanically reduced harvested pancreas, and (b) a supportive mammalian albumin substantially free of non-human microbiological agents, wherein (at least some stage in the method) the islets are associated with Sertoli cells.

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