Apparatus, method and computer program product for automated high-throughput sampling and data acquisition

• US 20040014227A1
• Filed: 03/14/2003
• Published: 01/22/2004
• Est. Priority Date: 01/04/2002
• Status: Abandoned Application

• Current Assignee: Nuada Pharmaceuticals, Inc.
• Original Assignee: Nuada Pharmaceuticals, Inc.
• Inventors: Polyakov, Valery R., Frederick, Erik D., Eichenbaum, Gary M., Geysen, Hendrik M.
• Application Number:
  US10/182,885
• Publication Number:
  US 20040014227A1
• Time in Patent Office: Days
• US Class Current: 436/43
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: G01N 30/20 using a sampling valve
• CPC Class Codes: G01N 30/24 Automatic injection systems
• CPC Class Codes: G01N 35/1009 Characterised by arrangements for controlling the aspiration or dispense of liquids
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

In a method, apparatus and computer program product for acquiring data from a sample, a sample or at least an initial portion thereof is transferred into an analytical instrument and the analytical instrument acquires data from the sample. While the analytical instrument is acquiring data, one or more properties of the sample are measured or the status of the system or instrumentation is considered. A determination is made as to whether the sample of the associated system or instrumentation meets one or more decision criteria (273) based on the one or more properties measured or on the status information obtained. A valve assembly (60) is_also provided that is adjustable to at least three modes operation, a sample injection/needle rinse mode, a sample loop load/instrument flush mode, and a sample loop flush/instrument flush mode.

Claims

1. A method for acquiring data from a sample, comprising the steps of:

(a) transferring at least an initial portion of a sample into an analytical instrument;

(b) causing the analytical instrument to acquire data from the initial portion of the sample;

(c) while the analytical instrument is acquiring data, measuring one or more properties of the initial portion of the sample;

(d) determining whether the initial portion of the sample meets one or more decision criteria based on the one or more properties measured; and

(e) in response to determining the initial portion of the sample to have failed to meet any of the one or more decision criteria, causing the analytical instrument to cease acquiring data from the initial portion of the sample, or in response to determining the initial portion of the sample to have met all of the one or more decision criteria transferring an additional portion of the sample into the analytical instrument and causing the analytical instrument to acquire data from the additional portion.

2. The method according to claim 1 wherein the initial portion of the sample is transferred into the analytical instrument from a sample reservoir.

3. The method according to claim 2 comprising the step of, prior to transferring the initial portion of the sample into the analytical instrument, loading the sample into the sample reservoir from a sample conduit.

4. The method according to claim 3 comprising the step of flushing the analytical instrument while the sample reservoir is being loaded.

5. The method according to claim 3 comprising the step of flushing the sample conduit while the initial portion of the sample is being transferred into the analytical instrument.

6. The method according to claim 5 wherein the sample conduit comprises a sampling probe.

7. The method according to claim 2 comprising the step of flushing the sample reservoir after determining whether the initial portion of the sample meets one or more decision criteria.

8. The method according to claim 7 comprising the step of flushing the analytical instrument while the sample reservoir is being flushed.

9. The method according to claim 1 comprising the steps of:

(a) transferring the sample into a sample loop from a sampling probe, wherein the initial portion of the sample is transferred into the analytical instrument from the sample loop; and

(b) rinsing the sampling probe while the initial portion of the sample is being transferred into the analytical instrument.

10. The method according to claim 1 comprising the step of, in response to determining the initial portion of the sample to have failed to meet any of the one or more decision criteria, causing a solvent to flow into the analytical instrument.

11. The method according to claim 1 comprising the step of, in response to determining the initial portion of the sample to have failed to meet any of the one or more decision criteria, preventing a remaining portion of the sample from being injected into the analytical instrument.

12. The method according to claim 11 comprising the step of discarding the remaining portion of the sample.

13. The method according to claim 11 comprising the step of diverting the remaining portion of the sample away from the analytical instrument.

14. The method according to claim 13 comprising the step of transferring the remaining sample to an apparatus.

15. The method according to claim 14 wherein the apparatus is another analytical instrument.

16. The method according to claim 11 comprising the step of altering an analytical method performed by the analytical instrument.

17. A computer program product comprising computer-executable instructions embodied in a computer-readable medium for acquiring data from a sample, the steps comprising:

(a) causing at least an initial portion of a sample to be transferred into an analytical instrument;

(b) causing the analytical instrument to acquire data from the initial portion of the sample;

(c) while the analytical instrument is acquiring data, measuring one or more properties of the initial portion of the sample;

(d) determining whether the initial portion of the sample meets one or more decision criteria based on the one or more properties measured; and

(e) in response to determining the initial portion of the sample to have failed to meet any of the one or more decision criteria, causing the analytical instrument to cease acquiring data acquisition from the initial portion of the sample, or in response to determining the initial portion of the sample to have met all of the one or more decision criteria transferring an additional portion of the sample into the analytical instrument and causing the analytical instrument to acquire data from the additional portion.

18. The computer program product according to claim 17 wherein the initial portion of the sample is transferred into the analytical instrument from a sample reservoir.

19. The computer program product according to claim 18 comprising the step of, prior to transferring the initial portion of the sample into the analytical instrument, loading the sample into the sample reservoir from a sample conduit.

20. The computer program product according to claim 19 comprising the step of flushing the analytical instrument while the sample reservoir is being loaded.

21. The computer program product according to claim 19 comprising the step of flushing the sample conduit while the initial portion of the sample is being transferred into the analytical instrument.

22. The computer program product according to claim 21 wherein the sample conduit comprises a sampling probe.

23. The computer program product according to claim 18 comprising the step of flushing the sample reservoir after determining whether the initial portion of the sample meets one or more decision criteria.

24. The computer program product according to claim 23 comprising the step of flushing the analytical instrument while the sample reservoir is being flushed.

25. The computer program product according to claim 17 comprising the steps of:

(a) transferring the sample into a sample loop from a sampling probe, wherein the initial portion of the sample is transferred into the analytical instrument from the sample loop; and

(b) rinsing the sampling probe while the initial portion of the sample is being transferred into the analytical instrument.

26. The computer program product according to claim 17 comprising the step of, in response to determining the initial portion of the sample to have failed to meet any of the one or more decision criteria, causing a solvent to flow into the analytical instrument.

27. The computer-program product according to claim 17 comprising the step of, in response to determining the initial portion of the sample to have failed to meet any of the one or more decision criteria, preventing a remaining portion of the sample from being injected into the analytical instrument.

28. The computer program product according to claim 27 comprising the step of discarding the remaining portion of the sample.

29. The computer program product according to claim 27 comprising the step of diverting the remaining portion of the sample away from the analytical instrument.

30. The computer program product according to claim 29 comprising the step of transferring the remaining sample to an apparatus.

31. The computer program product according to claim 30 wherein the apparatus is another analytical instrument.

32. The computer program product according to claim 27 comprising the step of altering an analytical method performed by the analytical instrument.

33. A method for acquiring data from a sample, comprising the steps of:

(a) loading a sample into a sample reservoir from a sample conduit;

(b) while the sample is being loaded, flushing an injection conduit;

(c) injecting at least a portion of the sample through the injection conduit into an analytical instrument;

(d) while the sample is being injected, flushing the sample conduit;

(e) flushing the sample reservoir;

(f) while the sample reservoir is being flushed, flushing the injection conduit; and

(g) repeating steps (a), (b), (c), and (d).

34. The method according to claim 33 comprising the steps of:

(a) providing a valve assembly in fluid communication with the sample reservoir, the sample conduit and the injection conduit;

(b) setting the valve assembly to a first position to enable the loading of the sample and the flushing of the injection conduit to occur simultaneously;

(c) setting the valve assembly to a second position to enable the injecting of the sample and the flushing of the sample conduit to occur simultaneously; and

(d) setting the valve assembly to a third position to enable the flushing of the sample and the flushing of the injection conduit to occur simultaneously.

35. A valve assembly for use in sample data acquisition, comprising:

(a) a sample loop; and

(b) a valve comprising an aspiration/dispensing port, a sampling port, a waste port, a pump-side port, and an instrument-side port, the valve being selectively adjustable to at least first, second and third positions, the first position defining a sample injection flow path and a sampling probe flushing path, the second position defining a sample loop loading flow path and an instrument flushing flow path, and the third position defining a sample loop flushing flow path.

36. The valve assembly according to claim 35 wherein:

(a) the sample injection flow path is directed from the pump-side injection port, through the sample loop, and to the instrument-side injection port;

(b) the sampling probe flushing path is directed from the aspiration/dispensing port to the sampling port;

(c) the sample loop loading flow path is directed from the sampling port to the sample loop;

(d) the instrument flushing flow path is directed from the pump-side injection port to the instrument-side injection port; and

(e) the sample loop flushing flow path is directed from the aspiration/dispensing port, through the sample loop, and to the waste port.

37. The valve assembly according to claim 36, wherein the sample loop comprises first and second openings, and the valve comprises a first sample loop port communicating with the first opening and a second sample loop port communicating with the second opening.

38. The valve assembly according to claim 36 comprising a valve structure and a movable valve body, wherein the valve structure comprises the aspiration/dispensing port, the sampling port, the waste port, the pump-side port, and the instrument-side port, and the valve body comprises a plurality of internal fluid passages selectively communicating with one or more of the ports of the valve structure at the first, second and third positions.

39. A valve assembly for use in sample data acquisition, comprising:

(a) a sample loop;

(b) a valve structure comprising an aspiration/dispensing port, a sampling port, a waste port, a pump-side port, and an instrument-side port;

(c) a valve body selectively adjustable to at least first, second and third positions, wherein the first position defines a sample injection flow path and a sampling probe flushing path, the second position defines a sample loop loading flow path and an instrument flushing flow path, and the third position defines a sample loop flushing flow path.

40. The valve assembly according to claim 39 wherein:

(a) the sample injection flow path is directed from the pump-side injection port, through the sample loop, and to the instrument-side injection port;

(b) the sampling probe flushing path is directed from the aspiration/dispensing port to the sampling port;

(c) the sample loop loading flow path is directed from the sampling port to the sample loop;

(d) the instrument flushing flow path is directed from the pump-side injection port to the instrument-side injection port; and

(e) the sample loop flushing flow path is directed from the aspiration/dispensing port, through the sample loop, and to the waste port.

41. The valve assembly according to claim 40, wherein the sample loop comprises first and second openings, and the valve structure comprises a first sample loop port communicating with the first opening and a second sample loop port communicating with the second opening.

42. The valve assembly according to claim 39 wherein the valve assembly has a dual-valve rotary configuration.

43. The valve assembly according to claim 39 wherein the valve assembly has a single-valve linear configuration.

44. The valve assembly according to claim 39 wherein the valve assembly has a single-valve rotary configuration.

45. A sample analysis system comprising:

(a) a robotic assembly;

(b) a sampling probe movably mounted to the robotic assembly;

(c) a sample loop; and

(d) a valve assembly mounted to the robotic assembly and comprising an aspiration/dispensing port, a sampling port, a waste port, a pump-side port, and an instrument-side port, the valve assembly being selectively adjustable to at least first, second and third positions, the first position defining a sample injection flow path and a sampling probe flushing path, the second position defining a sample loop loading flow path and an instrument flushing flow path, and the third position defining a sample loop flushing flow path.

46. The system according to claim 45 wherein:

(a) the sample injection flow path is directed from the pump-side injection port, through the sample loop, and to the instrument-side injection port;

(b) the sampling probe flushing path is directed from the aspiration/dispensing port, to the sampling port, and to the sampling probe;

(c) the sample loop loading flow path is directed from the sampling probe, to the sampling port, and to the sample loop;

(d) the instrument flushing flow path is directed from the pump-side injection port to the instrument-side injection port; and

(e) the sample loop flushing flow path is directed from the aspiration/dispensing port, through the sample loop, and to the waste port.

47. The system according to claim 45 comprising a reversible pump fluidly communicating with the aspiration/dispensing port.

48. The system according to claim 47 wherein the reversible pump is a syringe pump.

49. The system according to claim 45 comprising a waste receptacle fluidly communicating with the waste port.

50. The system according to claim 45 comprising an instrument pump fluidly communicating with the pump-side port.

51. The system according to claim 45 comprising an analytical instrument fluidly communicating with the instrument-side port.

52. The system according to claim 51 wherein the analytical instrument is a mass spectrometer.