Ultra high throughput sampling and analysis systems and methods

• US 20040014239A1
• Filed: 05/29/2003
• Published: 01/22/2004
• Est. Priority Date: 01/06/2000
• Status: Abandoned Application

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Inventors: Biondi, Sherri Ann, Parce, J. Wallace, Jensen, Morten J., Kopf-Sill, Anne R., Wolk, Jeffrey A.
• Application Number:
  US10/447,691
• Publication Number:
  US 20040014239A1
• Time in Patent Office: Days
• US Class Current: 436/180
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00367 capillary
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00587 High throughput processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01L 2200/025 : Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/027 : for microfluidic devices
• CPC Class Codes: B01L 2200/10 : Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 : Quality control, feedback systems
• CPC Class Codes: B01L 2300/0819 : Microarrays; Biochips
• CPC Class Codes: B01L 2300/0861 : Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2400/022 : droplet contacts the surface of the receptacle
• CPC Class Codes: B01L 2400/0406 : capillary forces
• CPC Class Codes: B01L 3/02 : Burettes; Pipettes
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 : characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: C12Q 2565/629 : being a microfluidic device
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 2001/028 : Sampling from a surface, swabbing, vaporising
• CPC Class Codes: G01N 2035/1034 : Transferring microquantities of liquid
• CPC Class Codes: G01N 2035/1037 : Using surface tension, e.g. pins or wires
• CPC Class Codes: G01N 2035/1039 : Micropipettes, e.g. microcapillary tubes
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 35/10 : Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Ultra-high throughput systems and methods are used for sampling large numbers of different materials from surfaces of substantially planar library storage components. The systems and methods typically employ: microfluidic devices having integrated capillary elements for carrying out the analysis of the sampled materials; library storage components, e.g., planar solid substrates, capable of retaining thousands, tens of thousands and hundreds of thousands of different materials in small areas; sensing systems for allowing rapid and accurate sampling of the materials by the microfluidic devices, and associated instrumentation for control and analysis of the overall operation of these systems.

Claims

1. A sample substrate array, comprising:

a substrate having a first surface; and

at least 100 separate test compound spots dried onto the first surface of the substrate, each test compound spot comprising a test compound and at least one excipient agent.

2. The substrate array of claim 1, wherein the at least 100 separate compounds spots are present at a density of at least about 100 compounds/cm² of substrate surface.

3. The substrate array of claim 1, wherein the at least 100 test compounds spots comprise at least 500 test compounds which are present at a density of at least about 500 compounds/cm² of substrate surface.

4. The substrate array of claim 1, wherein the at least 100 test compounds spots comprise at least 1000 test compounds which are present at a density of at least about 1000 compounds/cm² of substrate surface.

5. The substrate array of claim 1, wherein the first surface of the substrate has a surface area of at least 1 cm².

6. The substrate array of claim 1, wherein the first surface of the substrate comprises at least 200 different compounds spots reversibly immobilized thereon in discrete regions.

7. The substrate array of claim 1, wherein the first surface of the substrate comprises at least 1000 different compound spots reversibly immobilized thereon in discrete regions.

8. The substrate array of claim 1, wherein the surface of the substrate comprises at least 10,000 different compound spots reversibly immobilized thereon in discrete regions.

9. The substrate array of claim 1, wherein the first surface of the substrate comprises a metal.

10. The substrate array of claim 1, wherein the substrate comprises glass or quartz.

11. The substrate array of claim 1, wherein the first surface of the substrate is nonconductive.

12. The substrate array of claim 1, wherein the first surface of the substrate is selected from a metal oxide, SiO₂, Si₃N₄, siliconoxynitride and a polymeric material.

13. The substrate array of claim 1, wherein the first surface of the substrate is a polymeric material.

14. The substrate array of claim 13, wherein the polymeric material is selected from nitrocellulose, acrylic, polystyrene, parylene, polyvinylidine difluoride (PVDF), polysulfone, polyvinyl chloride, spun polypropylene, polytetrafluoroethylene (PTFE), and polycarbonate.

15. The substrate array of claim 1, wherein the at least one excipient agent is selected from a starch, dextran, glycol, polyethylene oxide, polyvinylpyrrolidone, a detergent, sucrose, fructose, maltose, and trehelose.

16. A method of fabricating a sample substrate array, comprising:

providing a substrate having a first surface;

depositing at least 100 separate test compounds on the first surface of the substrate; and

freeze drying each of the at least 100 separate test compounds on the first surface.

17. The method of claim 16, wherein the at least 100 separate compounds are present at a density of at least about 100 compounds/cm² of substrate surface.

18. The method of claim 16, wherein the at least 100 test compounds comprise at least 500 test compounds which are present at a density of at least about 500 compounds/cm² of substrate surface.

19. The method of claim 16, wherein the at least 100 test compounds comprise at least 1000 test compounds which are present at a density of at least about 1000 compounds/cm² of substrate surface.

20. The method of claim 16, wherein the first surface of the substrate has a surface area of at least 1 cm².

21. The method of claim 16, wherein the first surface of the substrate comprises at least 200 different compounds reversibly immobilized thereon in discrete regions.

22. The method of claim 16, wherein the first surface of the substrate comprises at least 1000 different compounds reversibly immobilized thereon in discrete regions.

23. The method of claim 16, wherein the first surface of the substrate comprises at least 10,000 different compounds reversibly immobilized thereon in discrete regions.

24. The method of claim 16, wherein the first surface of the substrate comprises a metal.

25. The method of claim 16, wherein the substrate comprises glass or quartz.

26. The method of claim 16, wherein the first surface of the substrate is nonconductive.

27. The method of claim 16, wherein the first surface of the substrate is selected from a metal oxide, SiO₂, Si₃N₄, siliconoxynitride and a polymeric material.

28. The method of claim 16, wherein the first surface of the substrate is a polymeric material.

29. The method of claim 28, wherein the polymeric material is selected from nitrocellulose, acrylic, polystyrene, parylene, polyvinylidine difluoride (PVDF), polysulfone, polyvinyl chloride, spun polypropylene, polytetrafluoroethylene (PTFE), and polycarbonate.