Methods and compositions for interferon therapy
First Claim
1. A pharmaceutical composition comprising gene delivery enhancing agent selected from the group consisting of SYN3 and a SYN3 homolog;
- and a gene delivery system, wherein the gene delivery system contains a gene for interferon and wherein the gene for interferon is operably linked to a genetic regulatory element which modulates expression of the gene.
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Abstract
Methods and pharmaceutical compositions for administering interferon therapy to tissues or organs having an epithelial cell layer are provided. A recombinant adenoviral vector encoding an interferon gene is administered to the target tissue or organ in combination with treatment with a delivery enhancing agent which increases the transduction of the cells of the target tissues or organs by the vector. The methods and combinations are useful in the treatment of cancers and other conditions responsive to interferon therapy. An exemplary method comprises the transurethral intravesical administration to the bladder of a therapeutically effective amount of a pharmaceutical composition comprising an adenoviral vector encoding alpha-interferon and SYN3 or a SYN3 homolog or analog. In the urinary bladder, as much as a 1,000 to 10,000 fold increase in interferon gene expression has been achieved by use of the combination of SYN3 with the recombinant adenoviral vector as compared to the use of the vector without SYN3.
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Citations
58 Claims
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1. A pharmaceutical composition comprising gene delivery enhancing agent selected from the group consisting of SYN3 and a SYN3 homolog;
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a gene delivery system, wherein the gene delivery system contains a gene for interferon and wherein the gene for interferon is operably linked to a genetic regulatory element which modulates expression of the gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 17, 18, 19, 20, 21, 22, 50)
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- 15. The composition of 13, wherein m and n are each 1.
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23. A method for delivering interferon gene therapy to a mammalian subject, said method comprising administering to the epithelial tissue or organ of the subject a therapeutically effective amount of a pharmaceutical composition comprising a gene delivery system wherein the gene for interferon is operably linked to a genetic regulatory element that modulates expression of the interferon gene and an agent selected from the group consisting of SYN3 and SYN3 homologues;
- wherein the agent enhances the delivery of the interferon gene to the tissue or organ.
- View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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51. A kit comprising:
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a first container containing SYN3 or a SYN3 homologue capable of enhancing the delivery of a gene delivery system; and
a second container containing the gene delivery system wherein the gene delivery system has an interferon gene operably linked to a genetic regulatory element modulating the expression of the interferon gene. - View Dependent Claims (52, 53, 54, 55, 56, 57, 58)
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Specification