Human obesity LIPIN3 polynucleotide and polypeptide sequences and methods of use thereof
First Claim
1. An isolated polynucleotide comprising (a) the sequence shown in Table 8, or its complement;
- (b) a polynucleotide that selectively hybridizes to the sequence shown in Table 8 relative to a known polynucleotide;
(c) a region of at least about 15 contiguous nucleotides, said region comprising nucleotide 1904 shown in Table 8;
or (d) a polynucleotide that selectively hybridizes to the sequence shown in Table 8 relative to a known polynucleotide.
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Abstract
The present invention relates to human LIPIN3 polynucleotides, which are involved in the control of mammalian body weight. The invention encompasses LIPIN3 polynucleotide sequences, including altered (mutant) LIPIN3 sequences, and fragments thereof, vectors and host cells comprising LIPIN3 polynucleotides, Lipin3 polypeptides, antibodies that specifically bind to Lipin3 polypeptide, arrays comprising LIPIN3 polynucleotides and polypeptides, and purified cells comprising an altered LIPIN3 polynucleotide. The invention also encompasses methods and compositions for the diagnosis and/or treatment of obesity and obesity related diseases, and for the identification of individuals susceptible to such diseases. The invention also encompasses methods of screening for compounds that alter LIPIN3 expression, preferably reducing LIPIN3 expression, and compounds that modulate the activity of the LIPIN3 gene product.
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Citations
38 Claims
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1. An isolated polynucleotide comprising (a) the sequence shown in Table 8, or its complement;
- (b) a polynucleotide that selectively hybridizes to the sequence shown in Table 8 relative to a known polynucleotide;
(c) a region of at least about 15 contiguous nucleotides, said region comprising nucleotide 1904 shown in Table 8;
or (d) a polynucleotide that selectively hybridizes to the sequence shown in Table 8 relative to a known polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 21)
- (b) a polynucleotide that selectively hybridizes to the sequence shown in Table 8 relative to a known polynucleotide;
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11. An isolated polypeptide comprising:
- a polypeptide comprising (i) a polypeptide having the sequence shown in Table 10;
or (ii) a region consisting at least about 5 contiguous amino acids, wherein said region includes amino acid 634 of Table 10. - View Dependent Claims (12, 13)
- a polypeptide comprising (i) a polypeptide having the sequence shown in Table 10;
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14. A method for detecting differential expression of a LIPIN3 polynucleotide in a test sample comprising:
- detecting a level of expression of (i) a polynucleotide comprising the sequence shown in Table 1;
(ii) a polynucleotide comprising the sequence shown in Table 7B;
or (iii) a region of a polynucleotide having the sequence shown in Table 1 or Table 7B, wherein said region is at least about 10 nucleotides in length. - View Dependent Claims (16, 17, 18, 19)
- detecting a level of expression of (i) a polynucleotide comprising the sequence shown in Table 1;
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15. A method for detecting obesity or obesity-related disorders associated with differential expression of a LIPIN3 polynucleotide comprising:
(a) detecting a level of expression of (i) a polynucleotide comprising the sequence shown in Table 1;
(ii) a polynucleotide comprising the sequence shown in Table 7B;
or (iii) a region of a polynucleotide having the sequence shown in Table 1 or Table 7B, wherein said region is at least about 10 nucleotides in length.
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22. A method for detecting differential expression of a LIPIN3 polypeptide in a test sample comprising:
- detecting a level of expression of (i) a polypeptide comprising the sequence shown in Table 9;
(ii) a polypeptide comprising the sequence shown in Table 7A;
or (iii) a region of the polypeptide shown in Table 9 or Table 7A, wherein the region is at least 10 contiguous amino acids in length. - View Dependent Claims (23, 24)
- detecting a level of expression of (i) a polypeptide comprising the sequence shown in Table 9;
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25. A method of screening for agents that reduce the expression of a LIPIN3 polynucleotide in a test cell sample comprising:
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contacting one or more cells possessing LIPIN3 overexpression with an antiobesity drug candidate;
measuring expression of;
(i) a polynucleotide comprising the sequence shown in Table 1;
(ii) a polynucleotide comprising the sequence shown in Table 7B;
or (iii) a region of a polynucleotide having the sequence shown in Table 1 or Table 7B, wherein said region is at least about 15 nucleotides in length;
and determining whether expression of the polynucleotide has decreased. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33)
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34. A method of screening for agents that reduce the expression of a Lipin3 polypeptide in a test cell sample comprising:
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contacting one or more cells possessing Lipin3 overexpression with an antiobesity drug candidate;
measuring expression of;
(i) a polypeptide comprising the sequence shown in Table 9;
(ii) a polypeptide comprising the sequence shown in Table 7A;
or (iii) a region of the polypeptide shown in Table 9 or Table 7A, wherein the region is at least 10 contiguous amino acids in length;
and determining whether Lipin3 expression has decreased. - View Dependent Claims (35, 36, 37, 38)
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Specification