Method for producing a microarray

• US 20040018613A1
• Filed: 07/09/2003
• Published: 01/29/2004
• Est. Priority Date: 07/16/2002
• Status: Abandoned Application

• Current Assignee: Nisshinbo Industries, Inc. (Kawasaki Heavy Industries Limited)
• Original Assignee: Nisshinbo Industries, Inc. (Kawasaki Heavy Industries Limited)
• Inventors: Shoji, Tomoaki
• Application Number:
  US10/616,160
• Publication Number:
  US 20040018613A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00364 Pipettes
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00533 essentially rectangular
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 3/0262 using touch-off at substrate or container
• CPC Class Codes: G01N 2035/1039 Micropipettes, e.g. microcapillary tubes

Abstract

Microarray is produced by the method comprising the steps of forming a droplet of a liquid sample containing a biological substance at a pouring port of a micropipette by ejecting a predetermined amount of the liquid sample from the micropipette, supporting the micropipette at a position where the droplet formed at the pouring port can contact with a substrate, and transferring the droplet formed at the pouring port to the substrate, thereby spotting the sample on the substrate. The microarray has a plurality of visually identifiable spots of samples on the substrate.

Claims

1. A method for producing a microarray comprising the steps of spotting a liquid sample containing a biological substance onto the substrate by using an automated dispensing device which is equipped with micropipette and automatically performs at least the operations of supporting the micropipette and ejecting a liquid sample stored in the micropipette, and drying the spotted sample so that the biological substance is fixed onto the substrate, wherein the method comprises the steps of:

forming a droplet of the sample at a pouring port of the micropipette by ejecting a predetermined amount of the sample from the micropipette,

supporting the micropipette at the position where the droplet formed at the pouring port can contact with the substrate and

transferring the droplet formed at the pouring port to the substrate, thereby spotting the sample onto the substrate.

2. The method according to claim 1, wherein the sample is spotted at a plurality of positions on the substrate by an automated dispensing device having a plurality of micropipettes.

3. The method according to claim 1 or 2, wherein the droplet is formed by ejecting 0.5 to 2.0 μ

l of the sample from the micropipette.