Biosensor

• US 20040020777A1
• Filed: 06/03/2003
• Published: 02/05/2004
• Est. Priority Date: 06/03/2002
• Status: Abandoned Application

• Current Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Yamamoto, Tomohiro, Yoshioka, Toshihiko, Miyamoto, Yoshiko, Hasegawa, Miwa
• Application Number:
  US10/452,936
• Publication Number:
  US 20040020777A1
• Time in Patent Office: Days
• US Class Current: 205/54
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

In a biosensor comprising an electrically insulating base plate, an electrode system including a working electrode and a counter electrode formed on the base plate, and a reaction layer formed on or in the vicinity of the electrode system, at least surface of the reaction layer is made porous, so as to provide a biosensor of good response characteristic in which a reaction layer containing an enzyme dissolves quickly into a small amount of a sample solution and the enzyme reaction is effectively utilized.

Claims

1. A biosensor comprising an electrically insulating base plate, an electrode system including a working electrode and a counter electrode formed on the base plate, and a reaction layer formed on or in the vicinity of the electrode system, at least a surface of the reaction layer being porous.

2. The biosensor according to claim 1, wherein the reaction layer contains at least an enzyme and an aggregate of fine particles having an average diameter of not smaller than 0.1 μ

m and not larger than 1 μ

m.

3. The biosensor according to claim 1, wherein the fine particles are made of a material selected from the group consisting of a polymer compound, ceramic, glass, diamond and carbon.

4. The biosensor according to claim 1, wherein the reaction layer further contains an electron mediator.

5. The biosensor according to claim 1, wherein a layer containing an electron mediator is formed in the vicinity of the reaction layer.

6. The biosensor according to claim 1, wherein the reaction layer contains cholesterol esterase, a surfactant and at least one of cholesterol oxidase and cholesterol dehydrogenase, and a layer containing an electron mediator is formed in the vicinity of the reaction layer.