Synthesis of highly conjugated polymers
First Claim
Patent Images
1. A method comprising:
- providing a substantially mono-activated steric hindrance molecule; and
reacting the substantially mono-activated steric hindrance molecule with a polymer, to provide a polymer-steric hindrance molecule copolymer having an elongated structure and having a degree of conjugation of 90% or greater.
3 Assignments
0 Petitions
Accused Products
Abstract
Improved methods for forming substituted polymers having a high degree of conjugation include the steps of activating a steric hindrance molecule under conditions that ensure substantially all the steric hindrance molecules are mono-activated and then reacting with a polymer. An improved method for recovering polypeptides substituted with steric hindrance molecules and having an elongate configuration removes impurities and volatiles without achieving complete dryness of the copolymer.
29 Citations
31 Claims
-
1. A method comprising:
-
providing a substantially mono-activated steric hindrance molecule; and
reacting the substantially mono-activated steric hindrance molecule with a polymer, to provide a polymer-steric hindrance molecule copolymer having an elongated structure and having a degree of conjugation of 90% or greater. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 25, 26, 27)
-
-
10. A method comprising:
-
providing a substantially mono-activated lanthanide complexing molecule; and
reacting the substantially mono-activated lanthanide complexing molecule with a polypeptide. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19)
-
-
20. A method comprising:
-
providing a solution containing diethylenetriamine pentaacetic acid and triethylamine;
cooling the solution to a temperature of about −
40°
C. or less;
adding isobutyl chloroformate to the cooled solution to form a mono-anhydride of diethylenetriamine pentaacetic acid;
reacting the mono-anhydride of diethylenetriamine pentaacetic acid with a polypeptide containing lysine residues, whereby at least 90% of the lysine residues are substituted with diethylenetriamine pentaacetic acid groups. - View Dependent Claims (21, 22, 23, 28)
-
-
24. A diethylenetriamine pentaacetic acid-ploysine conjugate having a degree of conjugation of about 95% or higher.
-
29. A method of purifying a conjugated polypeptide comprising the steps of:
-
subjecting a first composition to one or more purification processes without rendering the composition substantially dry, the first composition containing impurities and a polypeptide substituted with a steric hindrance molecule, the substituted polypeptide having an elongated configuration; and
recovering a purified composition containing the substituted polypeptide having an elongated configuration. - View Dependent Claims (30, 31)
-
Specification