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Method to assess quorum sensing potential of microbial communities

  • US 20040023254A1
  • Filed: 01/07/2003
  • Published: 02/05/2004
  • Est. Priority Date: 01/08/2002
  • Status: Abandoned Application
First Claim
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1. A method of detecting quorum sensing potential of a microorganism in a sample comprising:

  • (a) extracting nucleic acid from a sample comprising at least one type of microorganism;

    (b) performing a polymerase chain reaction using said nucleic acid wherein said polymerase chain reaction comprises a first oligonucleotide primer and a second oligonucleotide primer, wherein each of said primers comprises at least 15 nucleobases, wherein said primers anneal to a consensus sequence of a lux gene and homologs thereof, wherein the first eight nucleobases at the 3′

    end of said primers comprise a match to said consensus sequence with optional degeneracy at positions 4-8 from the 3′

    end of said primers;

    wherein said polymerase chain reaction results in amplified DNA fragments;

    (c) separating said amplified DNA fragments; and

    (d) determining whether any amplified DNA fragment corresponds to the size of a predicted product related to quorum sensing potential, thereby detecting quorum sensing potential of said microorganism in said sample.

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