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Method for multiple chemokine receptor screening for antagonists using RAM assay

  • US 20040023286A1
  • Filed: 07/30/2003
  • Published: 02/05/2004
  • Est. Priority Date: 06/07/2001
  • Status: Active Grant
First Claim
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1. A method for identifying a chemoattractant receptor antagonist, comprising:

  • incubating a cell population comprising first and second chemoattractant receptors;

    contacting the cell population with an inhibitory concentration of a ligand for the first chemoattractant receptor;

    contacting the cell population with an inhibitory concentration of a ligand for the second chemoattractant receptor;

    contacting the cell population with a candidate antagonist;

    assaying migration of the cell population, wherein migration identifies the candidate antagonist as an antagonist of at least one of the first and second chemoattractant receptors; and

    determining whether an identified antagonist is an antagonist for one of the first chemoattractant receptors, the second chemoattractant receptor, or both.

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