Method and reagent for the detection of proteins and peptides

• US 20040023292A1
• Filed: 10/18/2002
• Published: 02/05/2004
• Est. Priority Date: 10/19/2001
• Status: Abandoned Application

• Current Assignee: Ribozyme Pharmaceuticals, Inc. (Alnylam Pharmaceuticals Incorporated)
• Original Assignee: Ribozyme Pharmaceuticals, Inc. (Alnylam Pharmaceuticals Incorporated)
• Inventors: Seiwert, Scott, McSwiggen, James
• Application Number:
  US10/273,907
• Publication Number:
  US 20040023292A1
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92
• CPC Class Codes: G01N 33/6803 General methods of protein analysis not limited to specific proteins or families of proteins

Abstract

Methods and reagents for detecting proteins without the need for purified protein are disclosed. These methods can be used with a variety of protein detection technologies, including nucleic acid sensor molecules, aptamers, antibodies, combinatorial peptides and combinatorial proteins.

Claims

1. A method, comprising:

a. generating one or more peptide sequences from a predetermined protein,

b. generating a detection reagent that can recognize at least one of the peptide sequences of (a); and

c. contacting the detection reagent with a system under conditions suitable for the determination of the presence or absence of a target peptide in the system.

8. The method of any of claims 1-3, wherein the peptides are generated by fragmenting the predetermined protein in silico.

9. The method of any of claims 1-3, wherein the peptides are generated by fragmenting the predetermined protein in vitro.

2. A method comprising:

a. generating one or more known peptide sequences from a predetermined protein,

b. synthesizing at least one peptide from (a),

c. generating a detection reagent that can recognize the peptide from (b); and

d. contacting the detection reagent with a system under conditions suitable for the determination of the presence or absence of the peptide in the system.

3. The method according to claim 2 wherein the system is subjected to the fragmentation conditions of (a) before being contacted with the detection reagent.

4. The method according to claim 3 wherein the detection reagent is an aptamer.

5. The method according to claim 3 wherein the detection reagent is a nucleic acid sensor molecule.

6. The method according to claim 3 wherein the detection reagent is an antibody.

7. The method according to claim 3 wherein the detection reagent is a combinatorial peptide.