Device for chemical and biochemical reactions using photo-generated reagents

• US 20040023368A1
• Filed: 04/21/2003
• Published: 02/05/2004
• Est. Priority Date: 02/11/1998
• Status: Abandoned Application

• Current Assignee: University of Houston (University of Houston System), Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Gao, Xiaolian, Zhou, Xiaochuan, Gulari, Erdogan
• Application Number:
  US10/419,380
• Publication Number:
  US 20040023368A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00434 Liquid crystal masks
• CPC Class Codes: B01J 2219/00439 using micromirror arrays
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00689 : using computers
• CPC Class Codes: B01J 2219/00711 : Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 : Compounds covalently bound to a solid support
• CPC Class Codes: C07H 21/00 : Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07K 1/045 : using devices to improve synthesis, e.g. reactors, special vessels
• CPC Class Codes: C07K 1/047 : Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 40/12 : Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G03F 7/00 : Photomechanical, e.g. photolithographic, production of textured or patterned surfaces, e.g. printing surfaces; Materials therefor, e.g. comprising photoresists; Apparatus specially adapted therefor using photoresist structures for special production processes, see the relevant places, e.g. B44C, H01L, e.g. H01L21/00, H05K

Abstract

This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. Specifically, the method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

Claims

28. An apparatus for light controlled reactions comprising:

i) a substrate comprising a plurality of reaction sites; and

ii) an optical system for directing light to said substrate, said optical system comprising;

a light source and a computer-controlled spatial optical modulator for selectively irradiating a plurality of the reaction sites.

29. The apparatus of claim 28, wherein said spatial optical modulator is a digital micromirror device.

30. The apparatus of claim 29, wherein said digital micromirror device comprises a plurality of individual rocking micromirrors.

31. The apparatus of claim 28, wherein said spatial optical modulator is a reflective liquid crystal display device.

32. The apparatus of claim 28, wherein said spatial optical modulator is a transmissive liquid crystal display device.

33. The apparatus of claim 28, further comprising a computer configured to control the irradiation pattern.

34. The apparatus of claim 28 comprising at least one filter in-line with the light source, wherein said filter is configured to transmit a desired wavelength.

35. The apparatus of claim 28, wherein said substrate is transparent.

36. The apparatus of claim 28, further comprising a reagent manifold having one or more inlets and one or more outlets, said manifold configured to direct at least one reagent solution to the reaction sites.

37. The apparatus of claim 28, wherein said reaction sites are isolated reaction wells.

38. An apparatus for parallel synthesis of polymer arrays on a solid substrate comprising:

i) a solid substrate comprising isolated reaction sites;

ii) a light source;

iii) a digital optical projector for projecting light from said light source in a computer-controlled iradiation pattern onto selected reaction sites; and

iv) a reagent manifold having one or more inlets and one or more outlets, and configured to direct at least one reagent solution to the reaction sites.

39. The apparatus of claim 38, wherein said digital optical projector is a digital micromirror device.

40. The apparatus of claim 39, wherein said digital micromirror device comprises a plurality of individual rocking micromirrors.

41. The apparatus of claim 38, wherein said spatial optical modulator is a reflective liquid crystal display device.

42. The apparatus of claim 38, wherein said spatial optical modulator is a transmissive liquid crystal display device.

43. The apparatus of claim 38, further comprising a computer configured to control the irradiation pattern.

44. The apparatus of claim 38, comprising at least one filter in-line with the light source, wherein said filter is configured to transmit a desired wavelength.

45. The apparatus of claim 38, wherein said substrate is transparent.

46. The apparatus of claim 38, wherein said reaction sites are isolated reaction wells.

47. The apparatus of claim 38, wherein said light source is a mercury lamp.

48. The apparatus of claim 38, wherein said light source is a xenon lamp.

49. The apparatus of claim 38, wherein said light source is a halogen lamp.

50. The apparatus of claim 38, wherein said light source is a laser.

51. The apparatus of claim 38, wherein said light source is a light emitting diode.

52. An apparatus comprising:

i) a substrate comprising a plurality of isolated reaction sites;

ii) a light source;

iii) a digital micromirror device configured to direct light from said light source to selectively irradiate said plurality of isolated reaction sites in a computer-controlled irradiation pattern; and

iv) a reagent manifold comprising one or more inlets and one or more outlets and configured to deliver at least one reagent to said reaction sites.