Self-assembling peptide surfaces for cell patterning and interactions

• US 20040023414A1
• Filed: 12/11/2002
• Published: 02/05/2004
• Est. Priority Date: 06/25/1997
• Status: Abandoned Application

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation), Massachusetts Institute of Technology
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation), Massachusetts Institute of Technology
• Inventors: Zhang, Shuguang, Yan, Lin, Whitesides, George, Rich, Alexander
• Application Number:
  US10/317,838
• Publication Number:
  US 20040023414A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07K 17/14 Peptides being immobilised on, or in, an inorganic carrier
• CPC Class Codes: C07K 2/00 Peptides of undefined number of amino acids; Derivatives thereof
• CPC Class Codes: C07K 7/08 having 12 to 20 amino acids gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68
• CPC Class Codes: C12N 2535/10 : Patterned coating
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 2610/00 : Assays involving self-assembled monolayers [SAMs]
• CPC Class Codes: G01N 33/54353 : with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G03F 7/0002 : Lithographic processes using patterning methods other than those involving the exposure to radiation, e.g. by stamping
• CPC Class Codes: G03F 7/165 : Monolayers, e.g. Langmuir-Blodgett

Abstract

This invention describes self assembled monolayers (SAMs) manufactured by imprinting reactive peptides onto solid supports. The invention further relates to methods of preparing and using these improved SAMs.

Claims

1. A device comprising:

a solid support; and

an array of isolated regions on the support, the array comprising a layer of peptides, wherein the peptides are bound to the support by a bond between the support and a terminal amino acid in a preselected, reproducible pattern.

2. The device of claim 1 further comprising a background region surrounding the isolated regions, wherein the peptides are not bound to the background region.

3. The device of claim 2 wherein the background region comprises an inert compound.

4. A device comprising:

a solid support; and

an isolated region comprising a layer of peptides, wherein the peptides are bound to the support by a bond between the support and a terminal reactive group in a preselected, reproducible pattern.

5. The device of claim 4 comprising a plurality of isolated regions of a self-assembled monolayer, the plurality of regions defining an ordered array on the support.

6. A device comprising:

a solid support;

an isolated region comprising a self-assembled monolayer of peptides, wherein the peptides are bound to the support by a bond between the support and a terminal reactive group in a preselected, reproducible pattern; and

a background region surrounding the isolated region and comprising a compound which can react with the support.

7. A device for immobilizing at least one biological material in a specific and predetermined pattern comprising:

a surface,

an array of immobilization islands in a specific and predetermined pattern over the surface isolated from each other by at least one background region, the array of immobilization islands comprising a first self-assembled monolayer comprising at least one first functional group wherein the at least one first functional group is selected to biophilic, and wherein the first self-assembled monolayer comprises a monolayer of linear peptides, the at least one background region comprising a second self-assembled monolayer having a second functional group wherein the second functional group is selected to be biophobic.