Sort block and liquid collection device for sorting flow cytometer

• US 20040025602A1
• Filed: 08/08/2002
• Published: 02/12/2004
• Est. Priority Date: 08/08/2002
• Status: Active Grant

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Norton, Pierce O.
• Granted Patent: US 6,880,414 B2
• Time in Patent Office: Days
• US Class Current: 73/863.21
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 15/1492 within droplets
• CPC Class Codes: G01N 2015/1481 Optical analysis of particles within droplets sorting particles within droplets G01N15/1492

Abstract

A sort block for use with sorting flow analysis system. The sort block includes a fin extending into a sort stream in the sort block. Unsorted particles and aerosol are aspirated into the open top of the fin. When error is detected, collection baskets associated with the fin extend into the sort stream, block passage of particles to the collection containers and aspirate all droplets and aerosol to a waste or retrieval container. This sort block may include error monitors, such as a laser and camera combination, which monitor the flow stream and control the extension of the fin and basket type collector.

Claims

1. A cell sort block comprising:

a block body, having an open top, an open bottom and an elongate interior chamber;

a block face sealed over said body to enclose on four sides the interior chamber;

said chamber having a top chamber opening through which droplets may be introduced in a flow stream into said chamber and an open chamber bottom through which droplets leave the chamber and flow into collection containers;

a particle deflector disposed to deflect charged droplets that pass through said chamber;

a fin having an open fin top facing the housing top opening and a closed fin bottom defining a fin slot, said fin located between the particle deflector and the bottom opening and extending into a depth of said interior chamber such that deflected particles are deflected to one of two sides of said fin and undeflected particles pass into said open fin top; and

an aspiration source evacuating air through said fin slot, said aspiration source providing a negative air pressure within said chamber moving air within said interior chamber into said open fin slot.

2. The sort block of claim 1, further comprising a circuitous intake path on a surface of said block body top allowing a gas to move from outside the sort block to inside the interior chamber.

3. The sort block of claim 1, wherein the block face is clear.

4. The sort block of claim 1, further including an o-ring between said block face and said block housing.

5. The sort block of claim 1, wherein said fin is mounted on an actuator.

6. The sort block of claim 5, further comprising a pair of collection baskets mounted flanking the fin, wherein said actuator may move said collection baskets between a first position and a second position wherein in said first position said baskets are positioned out of the flow stream and in a second position said baskets are positioned in the flow stream blocking flow of droplets through said open chamber bottom.

7. The sort block of claim 6, further including an error sensor, wherein when an error is sensed by said error sensor the actuator is activated to move the fin and collection baskets to the second position, thereby collecting the flow stream emanating from the flow cell.

8. The sort block of claim 6, wherein said baskets are affixed to said fin such that both the baskets and the fin move as a single unit.

9. The sort block of claim 7, wherein said error sensor includes a laser directed into the flow stream and a camera monitoring the flow stream.

10. The sort block of claim 9, further including at least one filter positioned in front of a lens of said camera.

11. The sort block of claim 10, wherein said at least one filter comprises two filters disposed on a filter holder and said block further includes a filter actuator joined to said filter holder whereby actuation of said filter actuator moves a different filter in front of the camera.

12. The sort block of claim 8, wherein said error sensor is one of a group consisting of a flow cell monitor, a pressure monitor and a computer error sensor.

13. The sort block of claim 6, wherein the collection baskets each have a hole within the collection basket in communication with the aspiration source.

14. The sort block of claim 13, wherein collected droplets are recovered by a recovery line feeding to a recovered liquid container.

15. The sort block of claim 1, further comprising a collection container holder joined to said sort block body such that a plurality of containers placed in said container holders are held in the path of deflected droplets.

16. The sort block of claim 15, further comprising an o-ring between the block housing and the collection container holder such that an air-tight seal between said block housing and said collection container holder is formed.

17. The sort block of claim 1, further including a pivotable mount, said mount allowing the sort block to be mounted on a frame and pivot in relation to the position of a fixed flow cell.

18. A device for use with a sorting flow cytometer, said cytometer having a flow cell for analysis of particles, a droplet generator producing droplets containing said particles, a charging system and deflection plates for sorting charged droplets in a flow stream, an enclosed block body having an interior chamber through which said flow stream of droplets pass and a plurality of collection container for collecting deflected droplets at an end of said interior chamber, the device comprising:

a fin having an open end and a closed end, said fin positioned between said deflection plates and said collection containers and having said open end facing said deflection plates said open end oriented to collect undeflected droplets;

an aspirator connected to said fin such that air in front of said open end is evacuated through said open end;

a pair of baskets flanking said fin, said baskets having an open end and a closed end, wherein a front end of said fin extends beyond said baskets;

an actuator arm onto which said fin and baskets are mounted;

an actuator mechanically linked to said actuator arm such that said actuator arm may position said fin and baskets in a retracted position and an extended position, wherein when said device is used in said sorting flow cytometer in said retracted position said open end of said fin collects a central stream of unsorted particles and said baskets do not collect sorted particles, and when said fin end baskets are in an extended position said baskets are positioned to collect deflected particles while said fin continues to collect undeflected particles, thereby blocking droplets from flowing into said collection containers.

19. The device of claim 18, wherein said device further includes an error sensor, wherein when said error sensor detects a flow cytometer error said actuator activates and moves said fin and basket to the extended position.

20. The device of claim 19, wherein said sensor includes a light source illuminating the flow stream and a camera viewing droplets illuminated by said light source, wherein a change in droplet flow rate may be detected by said camera triggering actuator movement to said extended position.

21. The device of claim 19, wherein said sensor is selected from a group comprising a pressure sensor, an power flow sensor, an optical sensor, and a computer error sensor.

22. The device of claim 21, wherein said system for actuating said actuator includes an error sensor selected from the group comprising an optical error sensor, an electronics monitoring sensor, a pressure sensor and a computer error sensor.

23. The device of claim 21, wherein said system for actuating said actuator includes a manually activated switch in which a user activates the actuator.

24. The device of claim 21, further including a first o-ring between said block face and said block body and a second o-ring between said block body and said collection container holder.

25. The device of claim 21 further including a recovery container associated with said aspiration source such that droplets and aerosol collected by said baskets and fin are drawn into said recovery container.

26. The device of claim 21, wherein said block face is transparent.

21-1. A flow cytometer cell sorting device comprising:

an elongate block body enclosing three sides;

a block face fit onto said block body enclosing a fourth side to define a block chamber having an open top end and an open bottom end;

an electrostatic particle deflector disposed to deflect charged droplets in a droplet stream introduced through said block chamber producing a stream of undeflected droplets and one or more streams of deflected droplets;

a holder for droplet collection containers joined to said block body, said holder designed to hold at least two collection containers at a location to receive deflected droplets;

a fin disposed in said chamber between said deflector and said holder, the fin having a closed fin bottom and an open fin top facing said particle stream;

a pair of baskets flanking said fin;

an aspiration source drawing air through said open fin top;

an actuator arm onto which said fin and baskets are mounted; and

an actuator mechanically linked to said actuator arm such that said fin and baskets may be moved between a first and second position, wherein in said first position said fin collects undeflected droplets and aerosol in said open end while deflected droplets pass to the collection container and wherein in said second position said fin is further extended into a notch on said block face and said baskets are extended into said block chamber such that deflected, non-deflected and aerosol are collected and droplets do not pass to said collection containers; and

a system for actuating said actuator.