Novel integrated circuit chip for bioassays

• US 20040029109A1
• Filed: 03/25/2003
• Published: 02/12/2004
• Est. Priority Date: 03/26/2002
• Status: Active Grant

• Current Assignee: Vincogen Corporation
• Original Assignee: Vincogen Corporation
• Inventors: Lai, Derhsing
• Granted Patent: US 7,338,811 B2
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00567 Transponder chips
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G06K 19/0716 at least one of the integrated circuit chips comprising a sensor or an interface to a sensor
• CPC Class Codes: Y02A 90/10 Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Abstract

The invention is directed to novel IC chips containing substances used particularly in bioassays. Furthermore, the invention is directed to assay methods and kits using these chips.

Claims

1. An integrated circuit chip for use in an assay of a substance comprising:

a. a transponder encoded with information which is transmitted to a receiver in response to a radio signal and

b. at least one substance attached to said transponder

2. The chip according to claim 1, wherein the substance is covalently attached to said transponder.

3. The chip according to claim 1, wherein the substance is noncovalently attached to said transponder.

4. The chip according to claim 1, wherein the substance is a biological molecule.

5. The chip according to claim 4, wherein the biological molecule is selected from the group consisting of a nucleic acid, protein, antibody and at least one cell or subcellular component thereof.

6. The chip according to claim 4, wherein said biological molecule is a member of a phage display library.

7. The chip according to claim 4, wherein said biological molecule is a cell comprising a pathogen.

8. The chip according to claim 1, wherein said substance comprises a detectable moiety.

9. The chip according to claim 1, wherein said transponder comprises binary code.

10. The chip according to claim 1, wherein said transponder further comprises an electrode.

11. The chip according to claim 1, wherein said substance is contacted with said electrode.

12. The chip according to claim 1, wherein said substance is selected from the group consisting of a whole cell, subcellular particle, virus, prion, viroid, lipid, fatty acid, nucleic acid, polysaccharide, protein, lipoprotein, lipopolysaccharide, glycoprotein, peptide, cellular metabolite, hormone, pharmacological agent, tranquilizer, barbiturate, alkaloid, steroid, vitamin, amino acid, sugar, non biological polymer, synthetic organic molecule, organometallic molecule, inorganic molecule, biotin, avidin and streptavidin.

13. The chip according to claim 1, wherein said chip comprises at least one metal option.

16. A method for detecting binding of a first substance to a second substance comprising

a. incubating the integrated chip of claim 1, said chip comprising a transponder and a first substance attached to said transponder with a second substance for a time and under conditions for the first and second substances to bind;

b. analyzing the incubated mixture of step a to detect binding of said first substance and said second substance and

c. identifying the substance attached to said transponder.

17. The method according to claim 16, wherein the substance attached to said transponder is identified by decoding the information on said transponder.

18. The method according to claim 16, wherein said substance attached to said transponder is a biological sample.

19. The method according to claim 18, wherein said substance attached to said biological sample is selected from the group consisting of a cell, subcellular component, virus and tissue.

20. The method according to claim 16, wherein said substance attached to said transponder is a substance comprising a detectable moiety.

21. The method according to claim 16, wherein said substance is selected from the group consisting of DNA, RNA, protein, natural product and pharmacological agent.

22. The method according to claim 16, wherein said substance is an antibody.

23. The method according to claim 16, wherein said antibody is a monoclonal antibody.

24. The method according to claim 16, wherein said substance is a fusion protein.

25. The method according to claim 16, wherein said substance is a member of a display library.

26. The method according to claim 16, wherein said substance is a member of a phage display library.

27. The method according to claim 16, wherein said library is selected from the group consisting of a gene expression library, protein library and antibody library,

28. The method according to claim 16, wherein said substance is a ligand.

29. The method according to claim 16, wherein said detectable moiety is selected from the group consisting of a chromophore, fluorophore and luminescent agent.

30. The method according to claim 16 wherein said detectable moiety is a luminescent agent selected from the group consisting of a chemiluminescent, photoluminescent, bioluminescent and electrochemiluminescent agent.

31. The method according to claim 16, wherein said transponder further comprises an electrode.

32. The method according to claim 31, wherein said substance is attached to said electrode.

33. The method according to claim 32, wherein said substance is contacted with a substance having an electrochemiluminescent moiety.

34. The method according to claim 32, wherein said substance attached to said electrode is contacted with a substance containing an electrochemiluminescent moiety.

35. The method according to claim 16, wherein said substance containing a detectable moiety is selected from the group consisting of an antibody, a protein and a drug.

36. The method according to claim 16, wherein said substance attached to said transponder is a receptor.

37. The method according to claim 16, wherein said substance attached to said transponder is a ligand.

38. The method according to claim 16, wherein said substance binding to said substance attached to said transponder is a biological sample.

39. The method according to claim 38, wherein said biological sample is selected from the group consisting of a blood, serum, saliva, urine, tissue, and cell sample.

40. The method according to claim 16, wherein said substance attached to said transponder is identified with SPR technology.

41. The method according to claim 16, wherein said a detectable moiety is added to the mixture of step (a) before analyzing said mixture.

42. A method for detecting binding of a biological molecule to a substance comprising

a. incubating the integrated chip of claim 1, said chip comprising a transponder and a substance attached to said transponder with a substance labeled with a detectable moiety;

b. analyzing the incubated mixture of step a to detect binding of said biological molecule to said labeled substance and

c. identifying the biological molecule attached to said transponder.

43. A method for detecting binding of a biological molecule to a substance comprising

a. incubating the integrated chip of claim 1, said chip comprising a transponder and a substance having a detectable moiety attached to said transponder with a biological molecule;

b. analyzing the incubated mixture of step a to detect binding of said biological molecule to said substance attached to said transponder and

c. identifying the biological molecule attached to said transponder.

50. A method for obtaining the integrated circuit of claim 1, comprising attaching a substance to a transponder containing a tag specific to said transponder, said tag containing information which identifies said transponder and is identified by (i) activating said tag with radiowaves and (ii) decoding said information on said tag.

51. The method according to claim 50, wherein the attachment of said biological molecule to said transponder is facilitated with a conjugating agent.

52. The method according to claim 50, wherein said method further comprises contacting said substance with a detectable moiety.

53. A multiplex assay method comprising:

a. providing multiple integrated circuit chips of claim 1 which comprise a substance attached to said chips;

b. contacting said chips with two or more substances to determine if said substance binds to a substance on each of said chips;

c. analyzing the incubated mixture of step b to detect binding of said substance to said chips and

d. identifying the substances bound to said substance on said chip.

54. The method according to claim 53, wherein each integrated circuit chip comprises the same substance.

55. The method according to claim 53, wherein said substance on said chip is a biological sample.

56. The method according to claim 53, wherein said substance on said chip contains a detectable moiety.

57. The method according to claim 53, wherein each chip is contacted with more than one substance containing a detectable moiety.

58. The method according to claim 53, wherein each chip comprises the same biological molecule and each chip is contacted with more than one substance.

59. The method according to claim 53 wherein each chip comprises the same substance and each chip is contacted with one or more biological samples.

60. The method according to claim 53, which further comprises adding a detectable moiety to the mixture of step a.

14. A system for assaying biological molecules comprising:

a. a radio frequency identification system comprising;

(i) an antenna which emits a radio signal;

(ii) a transponder having a read-only tag, wherein said tag contains unique information and said transponder activated by said antenna of (i) and (iii) receiver which receives and decodes said information and

b. at least one substance attached to said transponder.

15. The system according to claim 14, wherein said antenna is attached to said transponder.

44. A method for detecting binding of a biological sample to a substance comprising

a. attaching said sample to a transponder containing a tag specific to said transponder, said tag containing information which identifies said transponder and is identified by (i) activating said tag with radiowaves and (ii) decoding said information on said tag;

b. contacting said attached sample of step a. with a substance to obtain a mixture;

c. analyzing the mixture of step b to determine binding of said sample with said substance;

d. decoding the tag on said transponder to identify the biological sample bound to said substance.

45. The method according to claim 44, wherein said biological sample is selected from the group consisting of tissue, one or more cells, virus particles and subcellular structures.

46. The method according to claim 44, wherein the labeled substance is a pharmacological agent.

47. The method according to claim 44, wherein said a detectable moiety is added to the mixture of step a before analyzing said mixture.

48. A method for detecting binding of a biological sample to a substance comprising

a. attaching said substance, to a transponder containing a tag specific to said transponder, said tag containing information which identifies said transponder and is identified by (i) activating said tag with radiowaves and (ii) decoding said information on said tag, wherein said substance attached to said transponder has a detectable moiety;

b. contacting said attached substance of step a with a biological sample to obtain a mixture;

c. analyzing the mixture of step b to determine binding of said sample with said substance;

d. decoding the tag on said transponder to identify the biological sample bound to said substance.

49. The method according to claim 48, wherein a detectable moiety is added to the mixture of step a before analyzing said mixture.

61. A method for performing a multiplex assay of biological molecules in a sample comprising:

a. providing an integrated circuit chip comprising (i) an antenna which emits a radio signal and (ii) a transponder having a read-only tag, wherein said tag contains unique information and said transponder activated by said antenna of (i);

b. ttaching a substance to said transponder in step a;

c. contacting said substance in step b with one or more substances to determine is said substance binds to each biological molecule on said chips;

d. analyzing the mixture of step c to determine binding of said substance which contacted said substance on said chip;

e. ecoding the tag on said transponder to identify the biological sample bound to said substance.

62. The method according to claim 61, which further comprises after step (c) and before step (d) separating the chips from one another.

63. The method according to claim 61, wherein said tag is decoded with a receiver.

64. A kit for assaying a biological molecule comprising a transponder comprising a tag with information which is transmitted to a receiver in response to a radio signal.

65. The kit of claim 64, which further comprises a library of substances.

66. The kit according to claim 65, wherein the library is a phage display library.

67. The kit according to claim 64, which further comprises a detectable moiety.

68. A kit for assaying a biological molecule comprising

a. a radio frequency identification system comprising:

(i) an antenna which emits a radio signal;

(ii) a transponder having a read-only tag, wherein said tag contains unique information and said transponder activated by said antenna of (i) and (iii) receiver which receives and decodes said information and

b. A detectable moiety.

69. An integrated circuit chip for use in an assay of a substance comprising:

a. a transponder encoded with binary code which is transmitted to a receiver in response to a radio signal and b. at least one substance attached to said transponder.

70. A system for assaying a substance comprising

a. a radio frequency identification system comprising:

(i) an antenna which emits a radio signal;

(ii) a transponder having a read-only tag, wherein said tag contains unique binary code and said transponder is activated by said antenna of (ii) and (iii) receiver which receives and decodes said binary code and

b. at least one substance attached to said transponder.