Regulation of human skin healing
First Claim
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1. A method of vascularizing an autologous human skin graft, comprising the step of:
- injecting the skin graft intradermally with a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of tPA, MEL-CAM, uPA, SCF, bFGF, IGF-1, and ET-3, whereby the skin graft becomes vascularized.
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Abstract
The invention relates to new compositions and methods for preparing vascularized dermal reconstructs, vascularized skin grafts, and for enhancing vascularization in situ in response to a variety of medical conditions.
29 Citations
57 Claims
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1. A method of vascularizing an autologous human skin graft, comprising the step of:
injecting the skin graft intradermally with a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of tPA, MEL-CAM, uPA, SCF, bFGF, IGF-1, and ET-3, whereby the skin graft becomes vascularized. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- 13. A skin graft system comprising an immunodeficient mouse having a human skin graft which has been injected intradermally with a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of tPA, MEL-CAM, uPA, SCF, bFGF, IGF-1, and ET-3.
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28. A method of inducing vascularization in a mammalian tissue, comprising the step of:
injecting into the tissue a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of tPA, MEL-CAM, uPA, SCF, bFGF, IGF-1, and ET-3. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. An artificial microvascularized dermal reconstruct comprising:
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a monolayer of human endothelial cells;
a matrix comprising a first layer which is adjacent to the monolayer of endothelial cells and a second layer which is adjacent to the first layer, wherein the first layer comprises collagen type I and the second layer comprises fibroblasts and collagen type I; and
interconnected microvascular spaces within the matrix. - View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48)
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49. A method for producing a dermal reconstruct in vitro, comprising the steps of:
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overlaying a monolayer of endothelial cells with a first layer comprising collagen type I; and
overlaying the first layer with a second layer comprising both collagen type I and fibroblasts to form a dermal reconstruct, wherein at least one cell type of said endothelial cells and said fibroblasts has been transduced using a vector comprising a polynucleotide encoding a polypeptide selected from the group consisting of VEGF, Ang 1, Ang 2, tPA, uPA, MEL-CAM, SCF, PDGF-A, VEGF-D, TGF-b1, VEGF-C, PDGF-B, bFGF, IGF-1 and ET-3. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57)
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Specification