Method for attaching oligonucleotide to solid support and the oligonucleotide array prepared by the method thereof

• US 20040033503A1
• Filed: 06/04/2003
• Published: 02/19/2004
• Est. Priority Date: 04/14/2000
• Status: Abandoned Application

• Current Assignee: GenoTech Corp.
• Original Assignee: GenoTech Corp.
• Inventors: Kim, Jae-Jong, Cha, Sun-Ho
• Application Number:
  US10/257,528
• Publication Number:
  US 20040033503A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target

Abstract

The present invention relates to a method for attaching oligonucleotide to a solid support and an oligonucleotide array prepared by said method. In the present invention, an oligonucleotide array is prepared by the steps of: preparing a hydrophobic attachment layer; spotting solution of oligonucleotide in which a hydrophobic group is bonded to one side of its terminal onto the hydrophobic attachment layer; and solidifying the hydrophobic attachment layer.

Claims

1. A method of attaching oligonucleotide onto a support, which comprises the steps of applying a hydrophobic attachment layer, which can be solidified under certain conditions, onto the support;

spotting a solution of oligonucleotide with its 3′

- or 5′

-terminal bonded to a hydrophobic group onto a certain portion of said attachment layer; and

solidifying said attachment layer.

3. The method of attaching oligonucleotide onto a support, according to claim 1 or 2, wherein said oligonucleotide bonded to a hydrophobic group is a PCR amplified product from a primer with its 5′

-terminal bonded to a hydrophobic group.

4. The method of attaching oligonucleotide onto a support, according to claim 1 or 2, wherein said attachment layer is of polymers formed by polymerization or condensation polymerization.

5. The method of attaching oligonucleotide onto a support, according to claim 1 or 2, wherein said hydrophobic group, in one or more, is selected from the group consisting of a dimethoxytrityl group, a pyrene group, and cholesterol.

6. The method of attaching oligonucleotide onto a support, according to claim 3, wherein said attachment layer is of polymers formed by polymerization or condensation polymerization.

7. The method of attaching oligonucleotide onto a support, according to claim 3, wherein said hydrophobic group, in one or more, is selected from the group consisting of a dimethoxytrityl group, a pyrene group, and cholesterol.

8. An oligonucleotide array made by a method according to one of claims 1-7.

2. A method of attaching oligonucleotide onto a support, which comprises the steps of fluidifying a solidified hydrophobic attachment layer as applied onto the support under certain conditions;

spotting a solution of oligonucleotide with its 3′

- or 5′

terminal bonded to a hydrophobic group onto a certain portion of said attachment layer; and

solidifying said attachment layer.