Multipotent neural stemcells from peripheral tissues and uses thereof
First Claim
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1. A multipotent stem cell substantially purified from skin of a mammal.
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Abstract
This invention relates to multipotent neural stem cells, purified from the peripheral nervous system of mammals, capable of differentiating into neural and non-neural cell types. These stem cells provide an accessible source for autologous transplantation into CNS, PNS, and other damaged tissues.
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Citations
43 Claims
- 1. A multipotent stem cell substantially purified from skin of a mammal.
- 15. A population of at least ten cells, wherein at least 30% of the cells are multipotent stem cells substantially purified from skin of a mammal.
- 17. A composition comprising (i) a multipotent stem cell substantially purified from skin of a mammal, and (ii) a pharmaceutically acceptable carrier, auxiliary or excipient.
- 18. A cell aggregate comprising at least ten multipotent stem cells derived from skin of a mammal, said multipotent stem cells capable of differentiating as dopaminergic neurons.
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21. A method of producing a population of at least ten cells, wherein at least 30% of the cells are multipotent stem cells substantially purified from skin of a postnatal mammal, or progeny of said multipotent stem cells, said method comprising:
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(a) providing said skin from said mammal;
(b) culturing said skin under conditions in which multipotent stem cells proliferate and in which at least 25% of the cells that are not multipotent stem cells die or attach to the culture substrate; and
(c) continuing culture step (b) until at least 30% of the cells are multipotent stem cells or progeny of said multipotent stem cells.
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22. A method of producing a population of at least ten cells, wherein at least 30% of the cells are multipotent stem cells substantially purified from skin of a postnatal mammal or progeny of said multipotent stem cells, said method comprising:
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(a) providing said skin from said mammal;
(b) culturing said skin under conditions in which multipotent stem cells proliferate and in which at least 25% of the cells that are not multipotent stem cells die or attach to the culture substrate;
(c) separating said multipotent stem cells from said cells that attach to said culture substrate; and
(d) repeating steps (b) and (c) until at least 30% of the cells are multipotent stem cells or progeny of said multipotent stem cells. - View Dependent Claims (23)
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- 24. A multipotent stem cell in the central nervous system of a mammal, said multipotent stem cell produced by a method of comprising transplanting a multipotent stem cell substantially purified from the skin of a mammal into the central nervous system of said mammal.
- 27. A kit comprising a multipotent stem cell substantially purified from skin of a mammal.
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29. A kit for the substantial purification of multipotent stem cells from skin of a mammal.
- 30. A method of treating a patient having a disease characterized by failure of a cell type, said method comprising administering to said patient a multipotent stem cell derived from a peripheral tissue, said multipotent stem cell capable of producing neurons and glia.
- 33. A method for treating a patient having a disease characterized by failure of a cell type, said method comprising admininstering to said patient a differentiated cell that is the progeny of a multipotent stem cell derived from skin.
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35. A method for making a neuron that expresses dopamine, said method comprising:
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(a) providing a multipotent stem cell derived from skin; and
(b) culturing said cell under conditions whereby said cell differentiates into a neuron that expresses dopamine.
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- 36. A method for making a cell other than a skin cell, said method comprising culturing a multipotent stem cell substantially purified from peripheral tissue of a mammal under conditions in which said cell differentiates as a cell other than a skin cell.
- 39. A substantially pure composition of multipotent stem cells, which multipotent stem cell differentiates into neural cell types and non-neural cell types.
Specification