Software for the display of chromatographic separation data

• US 20040034513A1
• Filed: 05/22/2003
• Published: 02/19/2004
• Est. Priority Date: 12/30/1997
• Status: Active Grant

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Gallagher, Steven J.
• Granted Patent: US 6,834,240 B2
• Time in Patent Office: Days
• US Class Current: 703/1
• CPC Class Codes: G01N 2030/625 by measuring reference material, e.g. carrier without sample
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 30/86 Signal analysis
• CPC Class Codes: G01N 30/8651 Recording, data aquisition, archiving and storage

Abstract

Techniques for displaying chromatographic data using a graphical user interface are provided. Chromatographic separation data that is a series of measurements for a sample at a scanning location over time can be displayed on a display device in a series of bands. Additionally, the series of bands for multiple samples can be aligned on the display device.

Claims

1. A computer implemented method of displaying chromatographic separation data, comprising:

receiving a series of measurements indicating presence of constituents in a sample at a scanning location over time; and

displaying the series of measurements for the sample as a series of bands.

2. The method of claim 1, further comprising displaying the series of measurements for the sample as a graph of measurements vs. time.

3. The method of claim 1, wherein the sample includes at least one marker and the series of measurements for the sample indicates presence of the at least one marker in the sample at a scanning location over time.

4. The method of claim 3, further comprising:

identifying at least one peak in the series of measurements for the sample that corresponds to the at least one marker; and

aligning the series of measurements for the sample so that any displayed bands that correspond to the at least one marker are aligned with a predetermined location.

5. The method of claim 3, further comprising:

identifying two peaks in the series of measurements for the sample that correspond to two markers; and

scaling the series of measurements for the sample so that the two displayed bands that correspond to the two markers are aligned with predetermined locations.

6. The method of claim 3, further comprising displaying bands that correspond to the constituents in the sample visually different than the bands that correspond to the at least one marker.

7. The method of claim 3, further comprising:

receiving input to invert the display of the series of bands; and

inverting the displayed series of bands.

8. A computer system, comprising:

a processor;

a display device coupled to the processor;

a computer readable medium coupled to the processor that stores a computer program that displays chromatographic separation data, the computer program including;

computer code that receives a series of measurements indicating presence of constituents in a sample at a scanning location over time; and

computer code that displays the series of measurements for the sample as a series of bands on the display device.

9. A computer program product that displays chromatographic separation data, comprising:

computer code that receives a series of measurements indicating presence of constituents in a sample at a scanning location over time;

computer code that displays the series of measurements for the sample as a series of bands; and

a computer readable medium that stores the computer codes.

10. The computer program product of claim 9, wherein the computer readable medium is selected from the group consisting of CD-ROM, floppy disk, tape, flash memory, system memory, hard drive, and data signal embodied in a carrier wave.

11. A computer implemented method of displaying chromatographic separation data, comprising:

receiving a series of measurements indicating presence of constituents and at least one marker in a first sample at a scanning location over time;

receiving a series of measurements indicating presence of constituents and at least one marker in a second sample at a scanning location over time;

displaying the series of measurements for the first sample as a series of bands;

identifying at least one peak in the series of measurements for the first sample that corresponds to the at least one marker;

identifying at least one peak in the series of measurements for the second sample that corresponds to the at least one marker;

scaling the series of measurements for the second sample so that the displayed bands that correspond to the at least one marker in the first and second samples are aligned when displayed; and

displaying the series of measurements for the second sample as a series of bands adjacent to the bands for the first sample.

12. The method of claim 11, wherein the series of measurements for the second sample being received after the series of measurements for the first sample.

13. The method of claim 11, wherein the series of measurements for the first and second samples include peaks that indicate the presence of the constituents and the at least one marker such that each peak corresponds to a displayed band.

14. The method of claim 11, wherein the first sample includes a standard ladder of macromolecular species.

15. The method of claim 11, further comprising displaying the series of measurements for the sample as a graph of measurements vs. time.

16. The method of claim 11, further comprising displaying bands that correspond to the constituents in the sample visually different than the bands that correspond to the at least one marker.

17. The method of claim 11, further comprising:

receiving input to invert the display of the series of bands for the first and second samples; and

inverting the displayed series of bands for the first and second samples.

18. A computer system, comprising:

a processor;

a display device coupled to the processor;

a computer readable medium coupled to the processor that stores a computer program that displays chromatographic separation data, the computer program including;

computer code that receives a series of measurements indicating presence of constituents and at least one marker in a first sample at a scanning location over time;

computer code that receives a series of measurements indicating presence constituents and at least one marker in a second sample at a scanning location over time;

computer code that displays the series of measurements for the first sample as a series of bands;

computer code that identifies at least one peak in the series of measurements for the first sample that corresponds to the at least one marker;

computer code that identifies at least one peak in the series of measurements for the second sample that corresponds to the at least one marker;

computer code that scales the series of measurements for the second sample so that the displayed bands that correspond to the at least one marker are aligned when displayed; and

computer code that displays the series of measurements for the second sample as a series of bands adjacent to the bands for the first sample.

19. A computer program product that displays chromatographic separation data, comprising:

computer code that receives a series of measurements indicating presence of constituents and at least one marker in a first sample at a scanning location over time;

computer code that receives a series of measurements indicating presence constituents and at least one marker in a second sample at a scanning location over time;

computer code that displays the series of measurements for the first sample as a series of bands;

computer code that identifies at least one peak in the series of measurements for the first sample that corresponds to the at least one marker;

computer code that identifies at least one peak in the series of measurements for the second sample that corresponds to the at least one marker;

computer code that scales the series of measurements for the second sample so that the displayed bands that correspond to the at least one marker are aligned when displayed;

computer code that displays the series of measurements for the second sample as a series of bands adjacent to the bands for the first sample; and

a computer readable medium that stores the computer codes.

20. The computer program product of claim 19, wherein the computer readable medium is selected from the group consisting of CD-ROM, floppy disk, tape, flash memory, system memory, hard drive, and data signal embodied in a carrier wave.

21. A computer implemented method of graphically presenting chromatographic separation data, comprising:

acquiring chromatographic data for a sample, the chromatographic data for the sample including a set of constituents and a set of markers;

determining a position of each marker in the chromatographic data in order to define a range of positions;

determining an intensity of each marker in the chromatographic data in order to define a range of intensities;

determining a position of each constituent in the chromatographic data by scaling the position to the range of positions;

determining an intensity of each constituent in the chromatographic data by scaling the position to the range of range of intensities; and

presenting the position and intensity of each constituent in the chromatographic data in a graphical format.

22. The method of claim 21, wherein the graphical format is a series of bands.

23. The method of claim 22, further comprising displaying bands that correspond to the set of constituents visually different than the bands that correspond to set of markers.

24. The method of claim 22, further comprising:

receiving input to invert the presentation of the series of bands; and

inverting the presented series of bands.

25. A computer system, comprising:

a processor;

a display device coupled to the processor;

a computer readable medium coupled to the processor that stores a computer program that graphically presents chromatographic separation data, the computer program including;

computer code that acquires chromatographic data for a sample, the chromatographic data for the sample including a set of constituents and a set of markers;

computer code that determines a position of each marker in the chromatographic data in order to define a range of positions;

computer code that determines an intensity of each marker in the chromatographic data in order to define a range of intensities;

computer code that determines a position of each constituent in the chromatographic data by scaling the position to the range of positions;

computer code that determines an intensity of each constituent in the chromatographic data by scaling the position to the range of range of intensities; and

computer code that presents the position and intensity of each constituent in the chromatographic data in a graphical format.

26. A computer program product that graphically presents chromatographic separation data, comprising:

computer code that acquires chromatographic data for a sample, the chromatographic data for the sample including a set of constituents and a set of markers;

computer code that determines a position of each marker in the chromatographic data in order to define a range of positions;

computer code that determines an intensity of each marker in the chromatographic data in order to define a range of intensities;

computer code that determines a position of each constituent in the chromatographic data by scaling the position to the range of positions;

computer code that determines an intensity of each constituent in the chromatographic data by scaling the position to the range of range of intensities;

computer code that presents the position and intensity of each constituent in the chromatographic data in a graphical format; and

a computer readable medium that stores the computer codes.

27. The computer program product of claim 26, wherein the computer readable medium is selected from the group consisting of CD-ROM, floppy disk, tape, flash memory, system memory, hard drive, and data signal embodied in a carrier wave.