Analyte detection system

• US 20040037746A1
• Filed: 03/12/2003
• Published: 02/26/2004
• Est. Priority Date: 10/12/2000
• Status: Abandoned Application

• Current Assignee: Biosensor Systems Design, Inc.
• Original Assignee: Biosensor Systems Design, Inc.
• Inventors: Bauer, Alan Joseph
• Application Number:
  US10/380,395
• Publication Number:
  US 20040037746A1
• Time in Patent Office: Days
• US Class Current: 422/98
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A sensor (700) for detecting analytes of interest in which natural or synthetic macromolecules (740) are immobilized on an electrically conductive base member (720) to insure that interaction of analyte with the macromolecules will lead to altered de novo electrical signals in a sensor circuit (720,770,198).

Claims

1. A sensor for detecting an analyte, comprising:

a base member having a conductive property, said base member defining a first conductive element;

a binding agent layer proximate said base member;

a semiconductive element proximate said base member; and

,

a second conductive element that is electrically contacted to said semiconductive element and said first conductive element;

wherein said base member and said binding agent layer define a sensor strip.

2. The sensor according to claim 1, further comprising a chemical entity bound to said base member and disposed between said base member and said binding agent layer.

3. The sensor according to any of claims 1-2, further comprising two equipotential leads coupling said sensor strip to a detection unit, wherein at least one of said equipotential leads is electrically contacted to said semiconductive element.

4. The sensor according to claim 1, wherein a work function of said second conductive element is less than a work function of said semiconductive element and said work function of said second conductive element is greater than a work function of said base member.

5. The sensor according to any of claims 1-4, wherein said second conductive element is an element of an electrode connected to a detection unit, said second conductive element being brought into contact with said semiconductive element, wherein said semiconductive element is an element of said sensor strip.

6. The sensor according to any of claims 1-5, further comprising a packaging layer disposed above said binding agent layer, said packaging layer being soluble in a medium that contains the analyte.

7. The sensor according to any of claims 1-5, wherein said semiconductive element is an organic compound and is physically associated with said base member on a first side of said base member, wherein said binding agent layer is immobilized on a second side of said base member.

8. The sensor according to any of claims 1-5, wherein said sensor strip comprises a plurality of sensor strips.

9. The sensor according to any of claims 1-5, wherein said semiconductive element is electroluminescent.

10. A method for detecting a predetermined analyte, comprising the steps of:

providing an electrically conductive base member, said base member defining a first conductive element;

forming a binding agent layer of macromolecules in proximity to said base member, wherein said macromolecules are capable of interacting at a level of specificity with said predetermined analyte,

disposing a semiconductive element proximate said base member, wherein said base member, said binding agent layer and said semiconductive element define a sensor strip;

exposing said predetermined analyte to said binding agent layer, and,

detecting an electrical current generated in a closed electrical circuit, said electrical current being responsive to a presence of said predetermined analyte, wherein said closed electrical circuit comprises said base member, said semiconductive element, and a second conductive element electrically contacted to said semiconductive element.

11. The method according to claim 10, further comprising the steps of:

binding a chemical entity to said base member, and

forming said binding agent layer proximate said chemical entity.

12. The method according to any of claims 10-11, wherein said step of detecting is performed by equipotentially coupling leads of a detection unit to said sensor strip, wherein one of said leads is coupled to said semiconductive element.

13. The method according to claim 12, wherein said step of coupling said detection unit is performed by contacting electrodes to said sensor strip and said semiconductive element, electrical passivity of said electrodes being maintained while performing said step of coupling.

14. The method according to claim 13, wherein said second conductive element is physically associated with one of said electrodes prior to performing said step of coupling.

15. The method according to any of claims 10-11, wherein a work function of said conductive element is less than a work function of said semiconductive element and said work function of said conductive element is greater than a work function of said base member.

16. The method according to any of claims 10-11, wherein said semiconductive element is an organic compound and is physically associated with said base member on a first side of said base member, wherein said binding agent layer is immobilized on a second side of said base member.

17. The method according to any of claims 10-11, further comprising the step of disposing a packaging layer above said binding agent layer, said packaging layer being soluble in a medium that contains said predetermined analyte.

18. The method according to any of claims 10-11, further comprising the step of generating photonic energy in said closed electrical circuit.

19. The method according to claim 18, wherein said step of detecting comprises detecting said photonic energy.

20. The method according to claim 19, wherein said photonic energy is detected remote from said sensor strip.

21. The method according to claim 10, wherein said sensor strip comprises a plurality of sensor strips.

22. A sensor for the detection of an analyte through generated electroluminescence, comprising:

an electrically conductive base member, said base member defining a first conductive element;

a binding agent layer proximate said base member;

an electroluminescent semiconductive element proximate said base member; and

a second conductive element that is electrically contacted to said base member and said semiconductive element.

23. The sensor according to claim 22, wherein a work function of said second conductive element is less than a work function of said semiconductive element and said work function of said second conductive element is greater than a work function of said base member.

24. The sensor according to claim 22, wherein said second conductive element is optically opaque.