Electroprocessed collagen and tissue engineering

• US 20040037813A1
• Filed: 05/28/2003
• Published: 02/26/2004
• Est. Priority Date: 02/25/1999
• Status: Active Grant

• Current Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.), Virginia Commonwealth University
• Original Assignee: Organogenesis Inc. (Organogenesis Holdings, Inc.)
• Inventors: Simpson, David G., Bowlin, Gary L., Carr, Marcus E., Stevens, Peter J., Wnek, Gary E., Rajendran, Saravanamoorthy, Matthews, Jamil A.
• Granted Patent: US 7,615,373 B2
• Time in Patent Office: Days
• US Class Current: 424/93.7
• CPC Class Codes: A61F 2/08 Muscles; Tendons; Ligaments sutures A61B17/04
• CPC Class Codes: A61K 2035/126 Immunoprotecting barriers, e.g. jackets, diffusion chambers
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 15/32 Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin A61L15/225 takes precedence
• CPC Class Codes: A61L 2430/30 for muscle reconstruction
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 31/041 Mixtures of macromolecular compounds
• CPC Class Codes: A61P 41/00 Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
• CPC Class Codes: B29C 41/006 using an electrostatic field for applying the material
• CPC Class Codes: B29C 67/0007 Manufacturing coloured articles not otherwise provided for, e.g. by colour change
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C08L 89/06 : derived from leather or skin , e.g. gelatin
• CPC Class Codes: C12N 2533/40 : Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2533/54 : Collagen; Gelatin
• CPC Class Codes: C12N 5/0012 : Cell encapsulation
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: D01D 5/0038 : the fibre formed by solvent evaporation, i.e. dry electro-spinning
• CPC Class Codes: D01F 1/10 : Other agents for modifying properties
• CPC Class Codes: D01F 4/00 : Monocomponent artificial filaments or the like of proteins; Manufacture thereof
• CPC Class Codes: Y10T 428/298 : Physical dimension
• CPC Class Codes: Y10T 442/2525 : Coating or impregnation functions biologically [e.g., insect repellent, antiseptic, insecticide, bactericide, etc.]
• CPC Class Codes: Y10T 442/60 : Nonwoven fabric [i.e., nonwoven strand or fiber material]
• CPC Class Codes: Y10T 442/614 : Strand or fiber material specified as having microdimensions [i.e., microfiber]

Abstract

The invention is directed to formation and use of electroprocessed collagen, including use as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed collagen may also be combined with other molecules in order to deliver substances to the site of application or implantation of the electroprocessed collagen. The collagen or collagen/cell suspension is electrodeposited onto a substrate to form tissues and organs.

Claims

1. Electrospun collagen fibers.

2. The electrospun collagen fibers of claim 1, wherein the electrospun collagen fibers have an average diameter of between about 30 nm and about 10 μ

m.

3. The electrospun collagen fibers of claim 1, wherein the electrospun collagen fibers induce differentiation of cells in vivo or in vitro when the electrospun collagen fibers and the cells are placed in conditions effective to allow interaction between the electrospun collagen fibers and the cells.

4. A matrix comprising the electrospun collagen fibers of claim 1.

5. The matrix of claim 4, wherein the matrix induces differentiation of cells in vivo or in vitro when the matrix and the cells are placed in conditions effective to allow interaction between the matrix and the cells.

6. The matrix of claim 4, wherein the matrix comprises pores having an average diameter of about 10 μ

m or less.

7. The matrix of claim 4, wherein the matrix comprises pores having an average area of between about 0.1 square μ

m and 100 square μ

m.

8. The matrix of claim 4, wherein the matrix has an elastic modulus of between about 2 MPa and about 10 MPa when hydrated.

9. A method of inducing cellular differentiation, comprising placing the electrospun collagen fibers of claim 1 and cells in vivo or in vitro under conditions effective to allow interaction between the electrospun collagen fibers and the cells.

10. A matrix comprising isolated collagen fibers having an average diameter of between about 30 nm and about 10 μ

m.

11. The matrix of claim 10, wherein the matrix comprises pores having an average diameter of about 10 μ

m or less.

12. The matrix of claim 10, wherein the matrix comprises pores having an average area of between about 0.1 square μ

m and 100 square μ

m.

13. A matrix comprising isolated collagen fibers wherein the matrix induces differentiation of cells in vivo or in vitro when the matrix and the cells are placed in conditions effective to allow interaction between the isolated collagen fibers and the cells.

14. The matrix of claim 13, wherein the matrix comprises pores having an average diameter of about 10/m or less.

15. The matrix of claim 13, wherein the matrix comprises pores having an average area of between about 0.1 square μ

m and 100 square μ

m.

16. A matrix comprising collagen fibers wherein the matrix comprises pores having an average diameter of about 10 μ

m or less.

17. A matrix comprising collagen fibers wherein the matrix comprises pores having an average area of between about 0.1 square μ

m and 100 square μ

m.