Sample generation for genotyping by mass spectrometry
First Claim
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1. A method for DNA genotyping by mass spectrometry, comprising the steps of:
- a. reduction of the complexity of the DNA sample, b. generation of allele specific products on the products generated in step a, wherein the generation of allele specific products in step b. is achieved by at least one method that uses (an) allele specific oligonucleotide(s) c. mass spectrometric analysis of the products generated in step b, wherein the mass spectrometric analysis in step c. is performed on the products generated in step b. without purification or separation from the reaction mixture.
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Abstract
The invention is drawn to a universal method for preparing DNA samples for genotyping of single nucleotide polymorphisms (SNP), deletions and insertions by mass spectrometry. The invention relates to a method for genotyping that comprises three steps: a) reducing the complexity of the genome, b) generating allele specific products, c) analyzing the products by mass spectrometry, without purifying the products obtained in step b).
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15 Claims
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1. A method for DNA genotyping by mass spectrometry, comprising the steps of:
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a. reduction of the complexity of the DNA sample, b. generation of allele specific products on the products generated in step a, wherein the generation of allele specific products in step b. is achieved by at least one method that uses (an) allele specific oligonucleotide(s) c. mass spectrometric analysis of the products generated in step b, wherein the mass spectrometric analysis in step c. is performed on the products generated in step b. without purification or separation from the reaction mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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