Spatially resolved enzyme-linked assay
First Claim
1. A method for assessing at least one quality parameter or at least one quantity parameter of at least one species of analytes in a sample comprising the steps of establishing a sample domain having at least one wall, arranging catalyst-analyte complexes between the at least one species of analytes and at least one catalyst in a manner allowing the analytes to move relative to the wall(s) of the sample domain, arranging a substrate in the sample domain, said substrate being capable of being converted into a product through catalysing by said catalyst, contacting the substrate with the catalyst-analyte complexes of individual analytes allowing a detectable amount of product to be produced, recording an image of the product related to individual analytes in the sample domain, correlating the image to the at least one quality parameter or at least one quantity parameter of the at least one species of analytes.
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Abstract
The present invention relates to a method of assessing at least one quality parameter and/or at least one quantity parameter of at lest one analyte wherein said at least one analyte is connected to a catalyst capable of catalysing a substrate into a product, whereby the analyte is assessed through detection of product produced around the analyte. More particularly the present invention relates to a method of assessing at least one quality parameter or at least one quantity parameter of at least one species of analytes in a sample comprising the steps of establishing a sample domain having at least one wall, arranging in the sample domain catalyst-analyte complexes between the at lest one species of analytes and at least one catalyst in a manner allowing the analytes to move relative to the wall(s) of the sample domain, arranging a substrate in the sample domain, said substrate being capable of being converted into a product through catalysation by said catalyst, contacting the substrate with the catalyst-analyte complexes of individual analytes allowing a detectable amount of product to be produced, recording an image of the product related to individual analytes in the sample domain, correlating the image to the at least one quality parameter or the at least one quantity parameter of the at least one species of analytes.
35 Citations
114 Claims
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1. A method for assessing at least one quality parameter or at least one quantity parameter of at least one species of analytes in a sample comprising the steps of
establishing a sample domain having at least one wall, arranging catalyst-analyte complexes between the at least one species of analytes and at least one catalyst in a manner allowing the analytes to move relative to the wall(s) of the sample domain, arranging a substrate in the sample domain, said substrate being capable of being converted into a product through catalysing by said catalyst, contacting the substrate with the catalyst-analyte complexes of individual analytes allowing a detectable amount of product to be produced, recording an image of the product related to individual analytes in the sample domain, correlating the image to the at least one quality parameter or at least one quantity parameter of the at least one species of analytes.
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102. The method according to claim 102, wherein the detector is positioned in a housing having an opening allowing the emitted signals to reach the detector(s).
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103. A system for the assessment of at least one parameter of analytes in a liquid sample, comprising
a device comprising a sample domain comprising an exposing domain, an inlet through which a volume of a liquid sample representing the analyte material can been introduced, and a flow system comprising at least a channel allowing at least a portion of the volume of the liquid sample to flow within the device, the device further comprising means to control the flow of liquid around a catalyst-analyte complex in the sample domain, a detection device comprising at least a first detector for quantitatively detecting spatial image data and a processor for processing the detected image presentation, the device and the detection device having means for arranging the device in relation to the detection device in a manner allowing electromagnetic signals from a sample in the exposing domain of the device to pass to the detection device and to form, in the detection device, a spatial image representation of the exposing domain.
- 104. A system according to claim 104, wherein the flow system additionally comprises a compartment or a flow channel part in or from which at least part of one or more reaction components initially loaded in the compartment or flow channel part is added to at least a portion of the volume of the liquid representing the sample.
- 106. A system according to claim 106, wherein the first light source comprises an excitation light source.
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107. A system according to claim 107, wherein the first light source and the detector are located on the same side of the exposing domain.
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108. A system according to claim 108, comprising a second light source and a second detector, located on the opposite side from the first light source and first detection means.
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110. The system according to claim 110, wherein the excitation light filter is essentially circular, such as essentially ring formed.
- 112. The use according to claim 112, wherein the individual is a human being.
Specification