Amino acids factory

• US 20040038391A1
• Filed: 02/05/2003
• Published: 02/26/2004
• Est. Priority Date: 02/06/2002
• Status: Abandoned Application

• Current Assignee: Green Earth Industries, LLC
• Original Assignee: Green Earth Industries, LLC
• Inventors: Pyntikov, Alexander V., Salerno, Salvatore P.
• Application Number:
  US10/358,135
• Publication Number:
  US 20040038391A1
• Time in Patent Office: Days
• US Class Current: 435/289.1
• CPC Class Codes: A23J 1/002 from animal waste materials A23J1/10 takes precedence
• CPC Class Codes: A23J 1/04 from fish or other sea animals
• CPC Class Codes: A23J 3/34 using enzymes
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 33/10 by centrifugation centrifuges in general B04B; Cyclones cyclones in general B04C
• CPC Class Codes: C12M 41/26 of pH
• CPC Class Codes: C12M 45/02 by mechanical forces; Stirring; Trituration; Comminuting crushing, pulverizing, disintegrating in general B02C
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor

Abstract

A method of production of enzymatic protein hydrolysate from cold water animals that takes place under continuous mixing and precisely controlled conditions until amino acids and very short peptides are produced. The enzymatic protein hydrolysate of the present invention is sterilized, fish-oil and solid particles are removed by means of centrifuging. The enzymatic protein hydrolysate contains up to 90% free amino acids, cold fish oil, hydroxilaptite (calcium orthophosphate), proteins, highly molecular peptides, vitamin, minerals and calcium salts of phosphorus. The production process involves mixing ground fish waste products with an enzyme source comprising cold-water fish guts and entrails, containing relatively aggressive enzymes that are effective in even mild (slightly alkaline) medium of water. The industrial method provides a greater degree of refining than existing technology through the use of acid and alkaline denaturation steps, in combination with advanced separation and drying technology. The new drying technology results in a higher-quality, less expensive mixture than was previously possible.

Claims

1. A proteolytic fermenter, comprising:

a mixer having a multi-ton capacity into which raw materials are fed;

a cutter conjoining said mixer;

a reactor connected to said mixer and said cutter, into which feeds a first alkaline PH adjuster;

a first centrifuge connected to said reactor, said first centrifuge producing an hydroxylapatite;

a first denaturing tank, connected to said first centrifuge, into which feeds a first acidic PH adjuster;

a second centrifuge connected to said first denaturing tank, said second centrifuge producing cold fish oil;

a second denaturing tank, connected to said second centrifuge, into which feeds a second alkaline PH adjuster;

a third centrifuge, connected to said second denaturing tank, said third centrifuge producing a calcium-based product;

a PH adjuster, connected to said third centrifuge, into which feeds a second acidic PH adjuster; and

a concentrator connected to said PH adjuster, wherein

said concentrator comprises a nutrient separator and at least one dryer.

2. The fermenter according to claim 1, wherein said raw materials further comprise:

a substrate of essentially whole marine animals;

ferments comprising marine animal entrails having a mass content that is essentially equivalent to the mass content of said substrate; and

purified water having a mass content that is essentially equivalent to the mass content of said animal protein.

3. The fermenter according to claim 2, wherein said marine animals consist of a single species of farmed cold-water fish such as Salmon, Trout, Halibut, Tappai, or Cod.

4. The fermenter according to claim 2, wherein said ferments are derived from a single organ, such as the stomach of a fish.

5. The fermenter according to claim 2, wherein said marine animals consist of a single species of cold-water wild catch, such as Cod, Mackerel, or Herring.

6. The fermenter according to claim 2, wherein said marine animals consist of a single species of Pellagic fish.

7. The fermenter according to claim 2, wherein said marine animals consist of Shrimp or Krill.

8. The fermenter according to claim 2, wherein said marine animals consist of Industry Fish, such as Blue Whiting.

9. The fermenter according to claim 2, wherein said concentrator produces a liquid-based enzymatic protein hydrolysate (EPH) consisting essentially of peptides, amino acids, vitamins, and minerals.

10. The fermenter according to claim 2, wherein said concentrator produces a powder-based enzymatic protein hydrolysate (EPH) consisting essentially of vitamins, minerals, and an amino acid mix.

11. The fermenter according to claim 2, wherein said nutrient separator separates said EPH into a nutrient package consisting essentially of a vitamin mix, a mineral mix, and an amino acid mix.

12. The fermenter according to claim 9, wherein said amino acid mix consists of taurine, aspartine, threonine, serine, glutamic acid, praline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylaline, OH-Pro, omithine, lysine, histidine, and arginine.

13. The fermenter according to claim 10, wherein said amino acid mix consists of taurine, aspartine, threonine, serine, glutamic acid, praline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylaline, OH-Pro, omithine, lysine, histidine, and arginine.

14. The fermenter according to claim 10, wherein said amino acid mix consists of taurine, aspartine, threonine, serine, glutamic acid, praline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylaline, OH-Pro, omithine, lysine, histidine, and arginine.

15. The fermenter according to claim 10, wherein said minerals comprise cadmium, lead, mercury, arsenic, natrium, potassium, calcium, phosphorus, magnesium, iron, zinc, copper, manganese, selenium, cobalt, molybdenum, and chromium.

16. The fermenter according to claim 15, further comprising the following vitamins:

thiamine, riboflavin, pantothenic acid, pyridoxine, nicotinic acid, biotin, folic acid, cyanocobalamine, meso-inosite, and vitamin E.

17. An enzymatic protein hydrolysate consisting of 70-90% of the free amino acids:

taurine, aspartine, threonine, serine, glutamic acid, praline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylaline, oxyhedral proline, omithine, lysine, histidine, arginine; and

5-25% of a mix of cadmium, lead, mercury, arsenic, natrium, potassium, calcium, phosphorous, magnesium, iron, zinc, copper, manganese, selenium, cobalt, molybdenum, and chromium.

18. A proteolytic fermenter for cold-water marine fish, comprising:

a mixer having a multi-ton capacity;

a raw material solution feeding into said mixer, said solution essentially comprising, by weight, 25% laboratory-derived enzymes having a hydrolyzing strength equivalent to its weight in cold-water marine fish entrails, 25% whole fish, 50% purified water, and a basic solution;

a cutter conjoining said mixer;

a fermentation reactor connected to said mixer and said cutter, into which feeds a first alkaline PH adjuster;

a first centrifuge connected to said reactor, said first centrifuge producing an hydroxylapatite;

a first denaturing tank, connected to said first centrifuge, into which feeds a first acidic PH adjuster;

a second centrifuge connected to said first denaturing tank, said second centrifuge producing cold fish oil;

a second denaturing tank, connected to said second centrifuge, into which feeds a second alkaline PH adjuster;

a third centrifuge, connected to said second denaturing tank, said third centrifuge producing phosphorous salts of calcium;

a PH adjuster, connected to said third centrifuge, into which feeds a second acidic PH adjuster;

a protein hydrolysate concentrator, connected to said PH adjuster, comprising a nutrient separator and a dryer;

wherein, said concentrator produces an enzymatic protein hydrolysate consisting essentially of taurine, aspartine, threonine, serine, glutamic acid, praline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylaline, OH-proline, ornithine, lysine, histidine, and arginine, bioactive peptides, and select vitamins and minerals.