Immunogen adherence inhibitor directed to lactic acid producing organisms and method of making and using it

• US 20040043020A1
• Filed: 09/08/2003
• Published: 03/04/2004
• Est. Priority Date: 07/15/1999
• Status: Active Grant

• Current Assignee: Bradley M. Mitteness, Peter Nash
• Original Assignee: Bradley M. Mitteness, Peter Nash
• Inventors: Mitteness, Bradley M., Nash, Peter
• Granted Patent: US 7,238,351 B2
• Time in Patent Office: Days
• US Class Current: 424/130.1
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: C07K 16/02 from eggs
• CPC Class Codes: C07K 16/12 against material from bacteria
• CPC Class Codes: C07K 16/1203 from Gram-negative bacteria
• CPC Class Codes: C07K 16/1275 from Streptococcus (G)
• CPC Class Codes: C07K 2317/23 from birds

Abstract

A microbial adherence inhibitor specific to lactic acid producing microorganisms, in the form of fowl egg antibodies is disclosed, along with the method of making it and methods of using it. The inhibitor functions by substantially preventing the attachment or adherence of colony-forming immunogens in the rumen and intestinal tracts of host food animals. The inhibitor is made by inoculating female birds with the immunogen, allowing time for an immune response in the female bird and then harvesting the eggs that contain antibodies to the immunogen. The egg contents can be dried or used as a liquid and added to the feed or water for the host animals. Dependent upon the particular immunogen with which the female bird is inoculated, the egg antibody is used to promote the growth of food animals by improving feed conversion rates by decreasing the lactic acid production caused by the presence of certain colony-forming organisms in the animals, and to substantially reduce or eliminate the incidence of species that have been linked to very high production of lactic acid which can result in reduced performance and in acute situations, dangerously low rumen pH levels. When high levels of lactic acid are present in the rumen, rumen ulcers can form. When rumen ulcers are present other bacteria such as Fusobacterium necrophorum can escape the rumen and cause liver abscesses or laminitis, which further reduce feed conversion efficiency. Colony forming immunogens such as Streptococcus bovis (a major lactic acid producer) and Fusobacterium necrophorum can both be targeted by antibodies to enhance feed efficiency.

Claims

1. A method of production of a microbial adherence inhibitor for administration to food animals to control the incidence of acidosis in food animals by preventing the adherence of colony-forming lactic acid producing immunogens in the rumen or intestinal tracts of said food animals, which method comprises:

a. inoculating female birds, in or about to reach their egg laying age, with a lactic acid producing immunogen;

b. allowing a period of time sufficient to permit the production in the birds of antibody to the lactic acid producing immunogen;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells.

2. The method of claim 1 wherein:

said colony forming immunogen is from the class consisting of Streptococcus bovis, Lactobacillus spp., and Fusobacterium necrophorum.

3. The method of claim 1 including:

drying the separated antibody-containing contents of said eggs.

4. The method of claim 3 wherein:

said colony forming immunogen is from the class consisting of Streptococcus bovis, Lactobacillus, spp. and Fusobacterium necrophorum.

5. The method of claim 3 including:

providing a dry feed carrier material, said drying of the separated antibody-containing contents of said eggs is achieved by coating the dry carrier material with the separated antibody-containing contents of said eggs.

6. The method of claim 5 wherein:

the dry feed carrier material is selected from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

7. The method of claim 1 including:

mixing the antibody-containing contents of the eggs with a liquid extender.

8. A method of production of a microbial adherence inhibitor for administration to food animals to control the incidence of acidosis in food animals by preventing the adherence of colony-forming lactic acid producing immunogen in the rumen or intestinal tracts of said food animals, where said immunogen is the SB antigen from Streptococcus bovis, said method comprising:

a. inoculating female birds, in or about to reach their egg laying age, with SB antigen from Streptococcus bovis;

b. allowing a period of time to permit the production in the birds and eggs laid by the birds of antibody to SN antigen from Streptococcus bovis;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said harvested eggs from the eggshells.

9. The method of claim 8 including:

drying said antibody-containing contents of the eggs.

10. The method of claim 9 including:

providing a dry feed carrier material, said drying of the separated antibody-containing contents of said eggs is achieved by coating the dry carrier material with the separated antibody-containing contents of said eggs.

11. The method of claim 10 wherein:

the dry feed carrier material is selected from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

12. The method of claim 8 including:

mixing the antibody-containing contents of the eggs with a liquid extender.

13. A method of production of a microbial adherence inhibitor for administration to food animals to control the incidence of liver abscesses in food animals by preventing the adherence of colony-forming immunogen in the rumen or intestinal tracts of said food animals, where said immunogen is the FN antigen from Fusobactarium necrophorum, said method comprising:

a. inoculating female birds, in or about to reach their egg laying age, with FN antigen from Fusobacterium necrophorum;

b. allowing a period of time to permit the production in the birds and eggs laid by the birds of antibody to FN antigen from Fusobacterium necrophorum;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said harvested eggs from the eggshells.

14. The method of claim 13 including:

drying said antibody-containing contents of the eggs.

15. The method of claim 14 including:

providing a dry feed carrier material, said drying of the separated antibody-containing contents of said eggs is achieved by coating the dry carrier material with the separated antibody-containing contents of said eggs.

16. The method of claim 15 wherein:

the dry feed carrier material is selected from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grain and beet pulp.

17. The method of claim 13 including:

mixing the antibody-containing contents of the eggs with a liquid extender.

18. A microbial adherence inhibitor for administration to food animals to control the incidence of acidosis caused by lactic acid producing immunogen in the rumen or intestinal tracts of said food animals by reducing the ability of the immunogen to multiply, produced by the method of:

a. inoculating female birds, in or about to reach their egg laying age, with a lactic acid producing immunogen;

b. allowing a period of time sufficient to permit the production in the birds of antibody to the lactic acid producing immunogen;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells.

19. The microbial adherence inhibitor of claim 18 wherein:

said colony forming immunogen is from the class consisting of Streptococcus bovis, Lactobacillus, spp. and Fusobacterium necrophorum

20. The microbial adherence inhibitor of claim 19 wherein the method includes:

drying the antibody-containing contents of the eggs.

21. The microbial adherence inhibitor of claim 19 including:

a dry feed carrier coated with the antibody-containing contents of the eggs.

22. The microbial adherence inhibitor of claim 21 wherein:

the dry feed carrier material is selected from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

23. The microbial adherence inhibitor of claim 19 including:

a liquid extender mixed with the antibody-containing contents of the eggs.

24. The microbial adherence inhibitor of claim 23 wherein:

said liquid extender is chosen from the group of liquid molasses and PBS.

25. A microbial adherence inhibitor for administration to food animals to control the incidence of acidosis caused by Streptococcus bovis immunogen in the rumen or intestinal tracts of said food animals by reducing the ability of the immunogen to multiply, produced by the method of:

a. inoculating female birds, in or about to reach their egg laying age, with SB antigen from Streptococcus bovis;

b. allowing a period of time sufficient to permit the production in the birds and eggs laid by the birds of antibody to SB antigen from Streptococcus bovis;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells.

26. The microbial adherence inhibitor of claim 25 wherein said method includes:

drying said antibody-containing contents of said eggs.

27. The microbial adherence inhibitor of claim 25 including:

a dry feed carrier material coated with the antibody-containing contents of said eggs.

28. The microbial adherence inhibitor of claim 27 wherein:

the dry feed carrier material is selected from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

29. The microbial adherence inhibitor of claim 25 including:

a liquid extender mixed with the antibody-containing contents of the eggs.

30. A microbial adherence inhibitor for administration to food animals to control the incidence of liver abscesses caused by Fusobacterium necrophorum immunogen in said food animals by reducing the ability of the immunogen to multiply, produced by the method of:

a. inoculating female birds, in or about to reach their egg laying age, with FN antigen from Fusobacterium necrophorum;

b. allowing a period of time sufficient to permit the production in the birds and eggs laid by the birds of antibody to FN antigen from Fusobacterium necrophorum;

c. harvesting the eggs laid by the birds;

d. separating the antibody-containing contents of said eggs from the shells.

31. The microbial adherence inhibitor of claim 30 wherein the method includes:

drying said antibody-containing contents of said eggs.

32. The microbial adherence inhibitor of claim 30 including:

a dry feed carrier material coated with the antibody-containing contents of said eggs.

33. The microbial adherence inhibitor of claim 32 wherein:

the dry feed carrier material is selected from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

34. The microbial adherence inhibitor of claim 30 including:

a liquid extender mixed with the antibody-containing contents of the eggs.

35. The microbial adherence inhibitor of claim 34 wherein:

said liquid extender is selected from the group of liquid molasses and PBS.

36. A method for substantially reducing or eliminating the incidence of lactic acid in food animals caused by the presence of lactic acid forming and liver abscess forming immunogens in the animal by inhibiting the ability of the immunogens to adhere to the rumen or intestinal tracts of the animal to reduce the ability of the immunogens to multiply, said method comprising:

a. inoculating female birds, in or about to reach their egg laying age, with lactic acid producing immunogens;

b. allowing a period of time sufficient to permit the production in the birds of antibody to the lactic acid producing immunogens;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells;

e. drying said separated antibody-containing contents of said eggs;

f. distributing the resulting dried egg antibody product substantially uniformly through an animal feed or water; and

g. supplying the resulting antibody-containing animal feed or water to food animals to substantially prevent adherence of the targeted immunogens to the rumen or intestinal tracts of the animals.

37. The method of claim 36 wherein:

said lactic acid forming immunogens are selected from the class consisting of Streptococcus bovis, Lactobacillus, spp. and Fusobacterium necrophorum.

38. The method of claim 37 including:

providing a dry feed carrier material, said drying of the separated antibody-containing contents of said eggs is achieved by coating the dry carrier material with the separated antibody-containing contents of said eggs.

39. The method of claim 38 wherein:

the dry feed carrier material is selected from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.

40. A method for substantially reducing or eliminating the incidence of lactic acid in food animals caused by the presence of lactic acid forming immunogens in the animal by inhibiting the ability of the immunogens to adhere to the rumen or intestinal tracts of the animal to reduce the ability of the immunogens to multiply, said method comprising:

a. inoculating female birds, in or about to reach their egg laying age, with lactic acid producing immunogens;

b. allowing a period of time sufficient to permit the production in the birds of antibody to the lactic acid producing immunogens;

c. harvesting the eggs laid by the birds; and

d. separating the antibody-containing contents of said eggs from the shells;

e. distributing the resulting egg mixture antibody product substantially uniformly through an animal feed or water; and

f. supplying the resulting antibody-containing animal feed or water to food animals to substantially prevent adherence of the targeted immunogens to the rumen or intestinal tracts of the animals.

41. The method of claim 40 including:

mixing the antibody-containing contents of said eggs with a liquid extender.

42. The method of claim 41 wherein:

said liquid extender is chosen from the group liquid molasses and PBS.