Method of producing stem cell lines
First Claim
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1. A method of producing a cell line from a pluripotent cell comprising:
- isolating a tissue from a first animal to be used as a feeder tissue;
maintaining the feeder tissue in contact with a culture medium;
contacting a pluripotent cell from a second animal with the feeder tissue;
incubating the pluripotent cell together with the feeder tissue in the culture medium; and
recovering a cell line derived from the pluripotent cell.
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Abstract
A method of producing a cell line from a pluripotent cell by isolating a portion of a feeder tissue from a first animal, maintaining the feeder tissue in contact with culture medium, contacting and incubating a pluripotent cell from a second animal together with the feeder tissue in the culture medium, and recovering a cell line wherein the cell line is derived from a pluripotent cell. This method can be used to prevent pluripotent cells from differentiating or aging in an in vitro setting by using fibroblast-rich feeder tissue slices. This method can also be used to produce differentiated cell lines suitable for use in transplantation.
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20 Claims
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1. A method of producing a cell line from a pluripotent cell comprising:
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isolating a tissue from a first animal to be used as a feeder tissue;
maintaining the feeder tissue in contact with a culture medium;
contacting a pluripotent cell from a second animal with the feeder tissue;
incubating the pluripotent cell together with the feeder tissue in the culture medium; and
recovering a cell line derived from the pluripotent cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of a preventing a stem cell from differentiating or aging in vitro, comprising:
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isolating a fibroblast-rich tissue from a first animal to be used as a fibroblast rich feeder tissue;
maintaining the fibroblast rich feeder tissue in contact with a culture medium;
contacting a pluripotent cell from a second animal with the fibroblast rich feeder tissue;
incubating the pluripotent cell with the fibroblast rich feeder tissue in the culture medium; and
recovering a cell line derived from the pluripotent cell;
wherein the recovered cell line is prevented from differentiating or aging in vitro. - View Dependent Claims (12, 13, 14)
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15. A method for producing a differentiated cell line suitable for use in transplantation comprising:
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selecting a portion of a tissue from a first animal to be used as a feeder tissue;
maintaining the feeder tissue in contact with a culture medium;
contacting a pluripotent cell from a second animal with the feeder tissue;
incubating the pluripotent cell together with the feeder tissue in the culture medium; and
recovering a differentiated cell line derived from the pluripotent cell;
wherein the differentiated cell line is suitable for use in transplantation. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification