Method of producing stem cell lines

• US 20040043482A1
• Filed: 01/03/2003
• Published: 03/04/2004
• Est. Priority Date: 01/04/2002
• Status: Abandoned Application

• Current Assignee: Kye-Hyung Paik
• Original Assignee: Kye-Hyung Paik
• Inventors: Paik, Kye-Hyung
• Application Number:
  US10/337,248
• Publication Number:
  US 20040043482A1
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: C12N 2502/085 eye cells
• CPC Class Codes: C12N 2502/1329 Cardiomyocytes
• CPC Class Codes: C12N 2502/14 hepatocytes
• CPC Class Codes: C12N 2502/256 Renal cells
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0618 Cells of the nervous system
• CPC Class Codes: C12N 5/0657 Cardiomyocytes; Heart cells
• CPC Class Codes: C12N 5/067 Hepatocytes
• CPC Class Codes: C12N 5/0686 Kidney cells

Abstract

A method of producing a cell line from a pluripotent cell by isolating a portion of a feeder tissue from a first animal, maintaining the feeder tissue in contact with culture medium, contacting and incubating a pluripotent cell from a second animal together with the feeder tissue in the culture medium, and recovering a cell line wherein the cell line is derived from a pluripotent cell. This method can be used to prevent pluripotent cells from differentiating or aging in an in vitro setting by using fibroblast-rich feeder tissue slices. This method can also be used to produce differentiated cell lines suitable for use in transplantation.

Claims

1. A method of producing a cell line from a pluripotent cell comprising:

isolating a tissue from a first animal to be used as a feeder tissue;

maintaining the feeder tissue in contact with a culture medium;

contacting a pluripotent cell from a second animal with the feeder tissue;

incubating the pluripotent cell together with the feeder tissue in the culture medium; and

recovering a cell line derived from the pluripotent cell.

2. The method of claim 1, wherein the first animal and the second animal are from the same species.

3. The method of claim 2, wherein the first animal and the second animal are the same individual.

4. The method of claim 1, wherein the first animal and the second animal are from different species.

5. The method of claim 1, wherein the pluripotent cell is selected from the group consisting of a totipotent cell, a stem cell, an embryonic germ cell, and a multipotent stem cell.

6. The method of claim 5, wherein tissue from which the pluripotent cell is selected from the group consisting of a brain tissue, a liver tissue, a heart tissue, a pancreas tissue, and a blood tissue.

7. The method of claim 1, wherein the pluripotent cell is isolated from a vertebrate or an invertebrate.

8. The method of claim 1, wherein the feeder tissue is isolated from an animal.

9. The method of claim 1, wherein the feeder tissue is isolated from a vertebrate or an invertebrate.

10. The method of claim 1, further comprising replacing the feeder tissue with fresh feeder tissue.

11. A method of a preventing a stem cell from differentiating or aging in vitro, comprising:

isolating a fibroblast-rich tissue from a first animal to be used as a fibroblast rich feeder tissue;

maintaining the fibroblast rich feeder tissue in contact with a culture medium;

contacting a pluripotent cell from a second animal with the fibroblast rich feeder tissue;

incubating the pluripotent cell with the fibroblast rich feeder tissue in the culture medium; and

recovering a cell line derived from the pluripotent cell;

wherein the recovered cell line is prevented from differentiating or aging in vitro.

12. The method of claim 11, wherein the fibroblast rich feeder tissue is isolated from a fresh granulation tissue in a chronic inflammatory tissue or a fibrosarcoma.

13. The method of claim 11, wherein the fibroblast rich feeder tissue is isolated from a vertebrate or an invertebrate.

14. The method of claim 11, further comprising replacing the feeder tissue with fresh feeder tissue.

15. A method for producing a differentiated cell line suitable for use in transplantation comprising:

selecting a portion of a tissue from a first animal to be used as a feeder tissue;

maintaining the feeder tissue in contact with a culture medium;

contacting a pluripotent cell from a second animal with the feeder tissue;

incubating the pluripotent cell together with the feeder tissue in the culture medium; and

recovering a differentiated cell line derived from the pluripotent cell;

wherein the differentiated cell line is suitable for use in transplantation.

16. The method of claim 15, wherein the feeder tissue is cultured in vitro.

17. The method of claim 15, wherein the pluripotent cell is isolated from a human.

18. The method of claim 15, further comprising selecting feeder tissue derived from a dopaminergic neuron.

19. The method of claim 15, wherein the differentiated cell line is suitable for use in a transplantation of a nerve cell for treatment of a neurodegenerative disease.

20. The method of claim 19, wherein the neurodegenerative disease is Alzheimer'"'"'s disease.