Polymer-coated substrates for binding biological molecules
First Claim
1. A substrate for supporting a biological array, the substrate comprising:
- a reactive surface to which a polymer coating can attach by covalent bonds;
an even coating of a polymer containing functional groups, which can reduce nonspecific binding of various biomolecules to a polymer-coated substrate surface.
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Accused Products
Abstract
A substrate, which that is capable of attaching biomolecules, and a method for preparing the substrate are provided. The substrate has a reactive surface that can covalently attach a polymer coating containing functional groups, which can reduce nonspecific binding of biomolecules to the surface for a biological array. Optionally, at least a portion of the substrate may be coated with an intermediate tie layer, which enhances the covalent bonding between the polymer coating with the underlying substrate. The present invention also pertains to a method that uses electrostatic blocking agents to reduce non-specific binding of proteins to a substrate, especially anhydride-modified surfaces.
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Citations
100 Claims
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1. A substrate for supporting a biological array, the substrate comprising:
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a reactive surface to which a polymer coating can attach by covalent bonds;
an even coating of a polymer containing functional groups, which can reduce nonspecific binding of various biomolecules to a polymer-coated substrate surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A method for preparing a substrate to support an array of biomolecules, the method comprising:
- providing a substrate of a suitable material;
preparing on the substrate a reactive surface for attaching a polymer coating; and
, applying an polymer coating in an even layer to the reactive surface of the substrate. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61)
- providing a substrate of a suitable material;
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62. A method for making a biological array, the method comprising:
- providing a substrate;
preparing a reactive surface on said substrate for attaching a polymer coating;
applying a polymer coating to the reactive surface of the substrate; and
, depositing biomolecules onto said polymer-coated surface. - View Dependent Claims (63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94)
- providing a substrate;
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95. A method for reducing non-specific binding of proteins to a surface of a biological array device, the method comprising:
- contacting said surface with a charged compound that has good non-specific binding properties itself, after attachment of a biomolecule to said surface, but before a detection step.
- View Dependent Claims (96, 97, 98, 99, 100)
Specification