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PCR compatible nucleic acid sieving matrix

  • US 20040045827A1
  • Filed: 09/10/2003
  • Published: 03/11/2004
  • Est. Priority Date: 03/20/2000
  • Status: Active Grant
First Claim
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1. A method of performing PCR and separating one or more PCR products, the method comprising:

  • (i) mixing one or more PCR reaction components with a sieving medium to provide a PCR sieving medium, wherein the sieving medium comprises a polymer solution, which polymer solution comprises less than about 0.5% polymer; and

    (ii) thermocycling the PCR sieving medium to produce one or more PCR products; and

    , (iii) separating the one or more PCR products by flowing the one or more PCR products through the sieving medium.

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