Sample introduction device

• US 20040047768A1
• Filed: 09/10/2002
• Published: 03/11/2004
• Est. Priority Date: 05/12/1999
• Status: Active Grant

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Inventors: Sullivan, Kevin J.
• Granted Patent: US 7,157,056 B2
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A sample introduction device is provided that is particularly useful in the critical care environment for the anaerobic withdrawal of small volumes of arterial blood from a sample collection syringe and introduction thereof into a sample chamber of a sensor for analysis. The sample introduction device includes a Luer fitting adapted to mate an outlet of the container in a concentric orientation with an input aperture of the sample chamber. A tubular probe is adapted for concentric placement with both the outlet and inlet aperture, with a first end thereof extending into the syringe and a second end adapted for attachment to an air supply. The probe has a predetermined diameter sufficient to provide an annular clearance between the probe and each of the outlet and inlet aperture. A predetermined volume of air is injected through the probe into the container to displace a predetermined volume of sample therefrom, through the annular clearance into the sample chamber. Injecting air into the syringe through the probe to displace the sample therefrom, rather than drawing the sample into the probe, advantageously reduces or eliminates the need for washing the interior of the probe to relatively reduce the use of wash reagents.

Claims

1. A sample introduction device adapted for introducing a sample into a sample chamber of a sensor assembly from a container maintained in fluid communicating relationship therewith, said sample introduction device comprising:

a probe of substantially tubular construction, having a first end adapted to extend into the container and a second end adapted for connection to a material volume supply;

wherein said probe is adapted to inject a predetermined volume of material into the container to displace a predetermined volume of the sample from the container into the sample chamber.

2. The sample introduction device as set forth in claim 1, wherein:

an outlet of the container is disposed in fluid communicating relation with an inlet aperture of the sample chamber;

said probe is adapted to extend through the inlet aperture and through the outlet into the container; and

said probe is sized and shaped to provide clearance between said probe and each of the inlet aperture and outlet sufficient to enable said predetermined volume of the sample to be displaced from the container into the sample chamber therethrough.

3. The sample introduction device as set forth in claim 2, wherein said probe is substantially cylindrical and is adapted for disposition concentrically within the outlet and the inlet aperture, said clearance being substantially annular.

4. The sample introduction device as set forth in claim 2, wherein said probe is adapted to extend in fluid tight engagement through a probe aperture disposed in a wall of the sample chamber.

5. The sample introduction device as set forth in claim 4, wherein said probe is adapted for being withdrawn from said container, said fluid tight engagement between the probe aperture and said probe being adapted to wipe the sample from the exterior of said probe during said withdrawal.

6. The sample introduction device as set forth in claim 5, wherein said probe is substantially cylindrical and is adapted for simultaneous disposition within the probe aperture, the outlet and the inlet aperture.

7. The sample introduction device as set forth in claim 2, further comprising a fitting adapted to mate the outlet of the container in fluid communicating relationship with the input aperture of the sample chamber.

8. The sample introduction device as set forth in claim 7, wherein said fitting is adapted to maintain the outlet of the container in physical contact with the sample chamber.

9. The sample introduction device as set forth in claim 8, wherein said fitting is integrally fastened to the sample chamber.

10. The sample introduction device as set forth in claim 1, wherein said material is selected from the group consisting of a liquid, a paste, solid beads or granules, or gas.

11. The sample introduction device as set forth in claim 10, wherein said material is chemically inert.

12. The sample introduction device as set forth in claim 1, wherein said material comprises air.

13. A test apparatus for determining analyte content of a test sample, said test apparatus comprising:

the sample introduction device as set forth in claim 1;

the sensor assembly having at least one sample chamber; and

the container adapted for being maintained in fluid communicating relationship with said at least one sample chamber.

14. The test apparatus as set forth in claim 13, wherein said sensor assembly further comprises a multiple single use sensor assembly having a plurality of single use sample chambers disposed therein, said container and said sample introduction device adapted for being selectively engaged with each of said plurality of single use sample chambers for selectively introducing a sample therein.

15. A method of operating a sensor, comprising the steps of:

(a) maintaining a sample container in fluid communicating relationship with the sample chamber; and

(b) injecting a predetermined volume of gas into the sample container, wherein a predetermined volume of a sample disposed within the sample container is displaced by the gas from the container into the sample chamber.

16. The method as set forth in claim 15, wherein said injecting step (b) further comprises the steps of:

(c) extending the first end of a probe of substantially tubular construction, into the container; and

(d) supplying gas from a gas supply to a second end of the probe, wherein the gas is fed through the probe into the container.

17. The method as set forth in claim 16, wherein said extending step (c) further comprises the step of extending the first end of the probe through an inlet aperture of the sample chamber and into the container through an outlet thereof, the probe being sized and shaped to provide clearance between the probe and each of the inlet aperture and outlet sufficient to enable the predetermined volume of the sample to be displaced from the container into the sample chamber therethrough.

18. The method as set forth in claim 16, wherein the probe is adapted to extend in fluid tight engagement through a probe aperture disposed in a wall of the sample chamber.

19. The method as set forth in claim 18, further comprising the step of:

(e) withdrawing the probe, wherein the probe aperture serves to wipe sample fluid from the exterior of the probe.

20. The method as set forth in claim 19, wherein said withdrawing step (e) further comprises the step of withdrawing the probe from the probe aperture.

21. The method as set forth in claim 19, further comprising the steps of:

(f) measuring predetermined parameters of the sample disposed in the single use sample chamber; and

(g) discarding the sensor assembly with sample fluid disposed within the sample chamber.

22. The method as set forth in claim 19, wherein said withdrawing step (e) further comprises the steps of:

withdrawing the probe from the sample fluid disposed in the container; and

injecting a predetermined volume of gas into the probe, wherein the sample fluid disposed within the sample chamber is displaced therefrom.

23. The method as set forth in claim 22, wherein the sample fluid disposed within the sample chamber is displaced into a waste receptical.

24. The method as set forth in claim 23, further comprising the step of discarding the sensor assembly with sample fluid disposed within the sample chamber.

25. A sample introduction device adapted for introducing a sample from a container into a sample chamber of a sensor, said sample introduction device comprising:

a fitting adapted to mate an outlet of the container in fluid communicating relationship with an input aperture of the sample chamber;

a probe of substantially tubular construction, having a first end and a second end, said probe adapted to extend in a fluid tight manner through a probe aperture disposed in a wall of the sample chamber, through the input aperture and through the outlet wherein said first end extends into the container, said second end being adapted for connection to a gas supply;

said probe being sized and shaped to provide fluid communicating clearance between said probe and each of the input aperture and the outlet; and

said probe adapted for communicating a predetermined volume of gas therethrough into the container;

wherein said predetermined volume of gas serves to displace a predetermined volume of the sample from the container through the clearance into the sample chamber.