Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
First Claim
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1. A composition, comprising:
- a first component comprising a membrane;
a cationic membrane permeable dye; and
, an anionic membrane permeable redistributing dye.
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Abstract
Transmembrane potential measurement methods using cationic dyes, and anionic dyes are provided. Compositions of the cationic and anionic dyes and microfluidic systems which include the dyes and membranes are provided in conjunction with processing elements for transmembrane potential measurements.
64 Citations
72 Claims
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1. A composition, comprising:
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a first component comprising a membrane;
a cationic membrane permeable dye; and
,an anionic membrane permeable redistributing dye. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of generating a signal output which is sensitive to membrane potential, the method comprising:
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providing a first component comprising one or more membranes;
adding a cationic membrane permeable nucleic acid staining dye to the first component; and
,monitoring a first signal output from the cationic membrane permeable nucleic acid staining dye, wherein the first signal output is correlated with the transmembrane potential across the one or more membranes. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A method of producing a signal which is dependent on transmembrane potential, the method comprising:
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flowing a first mixture comprising one or more membranes and one or more voltage sensitive dyes through a first channel region; and
,monitoring at least a first signal output from at least one of the voltage sensitive dyes, thereby producing a signal which is dependent on the transmembrane potential across the one or more membranes. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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42. A method of generating an optical signal which is sensitive to transmembrane potential, the method comprising:
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providing a first component comprising one or more membrane;
adding a cationic membrane permeable redistributing dye to the first component;
adding an anionic membrane permeable redistributing dye to the first component; and
,measuring a first signal output from the cationic dye and a second signal output from the anionic dye, wherein one or more of the first and second signal outputs comprises an optical signal output, thereby generating the optical signal which is sensitive to the transmembrane potential. - View Dependent Claims (43, 44, 45, 57)
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46. A method of generating an optical signal which is dependent on transmembrane potential, the method comprising:
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providing a first component comprising one or more membrane;
adding at least a first membrane permeable redistributing dye to the first component, wherein the first membrane permeable redistributing dye comprises an ion;
measuring one or more signal output from the first redistributing dye before an equilibrium dye distribution is established, which one or more signal output comprises at least one optical signal output, thereby providing the optical signal which is dependent on transmembrane potential. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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58. A microfluidic device for monitoring transmembrane potential, the microfluidic device comprising:
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a body structure having at least one microscale cavity disposed therein;
a target source of a first composition comprising at least one membrane, which target source is fluidly coupled to the at least one microscale cavity; and
,a source of one or more voltage sensitive dyes which source is fluidly coupled to the at least one microscale cavity, wherein, during operation of the device, the first composition is contacted to the one or more voltage sensitive dyes in the at least one microscale channel. - View Dependent Claims (59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72)
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Specification