Method for the expression of biosynthetic genes in plant seeds using multiple expression constructs
First Claim
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1. An expression cassette with a structure selected from the group consisting of:
- a) L1-promoter-structural gene-L2, b) L1-promoter-structural gene-L2-L1-promoter-structural gene-L2, c) L1-promoter-structural gene-L2-L1-promoter-structural gene-L2-L1-promoter-structural gene-L2, where L1, L2, promoter and structural gene have the following meanings;
L1=SEQ ID NO;
32 or a sequence comprising equivalent restriction cleavage sites, L2=independently of one another SEQ ID NO;
33, 34 or 35 or sequences comprising equivalent restriction cleavage sites, promoter=plant promoter structural gene=a nucleic acid sequence which can be expressed in plants.
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Abstract
The present invention relates to expression cassettes, to their combination and vectors comprising the expression cassettes comprising plant promoters with an expression specificity for plant seeds, in particular linseed, and to the use of these expression cassettes or vectors for the recombinant expression of heterologous genes in plants. The invention furthermore relates to transgenic plants which have been transformed with these expression cassettes or vectors, to cultures, parts or transgenic propagation material derived therefrom, and to their use as food, feed or seed, pharmaceuticals, fine chemicals or as industrial feedstock.
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Citations
17 Claims
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1. An expression cassette with a structure selected from the group consisting of:
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a) L1-promoter-structural gene-L2, b) L1-promoter-structural gene-L2-L1-promoter-structural gene-L2, c) L1-promoter-structural gene-L2-L1-promoter-structural gene-L2-L1-promoter-structural gene-L2, where L1, L2, promoter and structural gene have the following meanings;
L1=SEQ ID NO;
32 or a sequence comprising equivalent restriction cleavage sites,L2=independently of one another SEQ ID NO;
33, 34 or 35 or sequences comprising equivalent restriction cleavage sites,promoter=plant promoter structural gene=a nucleic acid sequence which can be expressed in plants. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification