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Capture and detection format versatility for dipstick assays

  • US 20040053255A1
  • Filed: 04/07/2003
  • Published: 03/18/2004
  • Est. Priority Date: 07/07/2000
  • Status: Active Grant
First Claim
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1. A method for testing for the presence of target nucleic acid in a sample solution which comprises:

  • a) providing a chromatographic strip having a contact end for contacting the sample solution and a universal capture probe immobilised at a capture zone of the chromatographic strip remote from the contact end;

    b) providing a hook capture probe capable of hybridising to a first region of the target nucleic acid and to the universal capture probe;

    c) incubating the sample solution with the hook capture probe under conditions for hybridisation of the hook capture probe to the first region of target nucleic acid in the sample solution;

    d) contacting the sample solution with the contact end of the chromatographic strip so that a complex formed between the hook capture probe, the detection probe and target nucleic acid can travel by capillary action to the capture zone and bind to the capture zone by interaction between the hook capture probe of the complex and the universal capture probe immobilised at the capture zone; and

    e) detecting for target nucleic acid at the capture zone.

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