Detection signal and capture in dipstick assays
First Claim
Patent Images
1. A kit for testing for the presence of target nucleic acid in a sample solution which comprises:
- i) a dipstick comprising;
a chromatographic strip having a contact end for contacting the sample solution;
a capture moiety, immobilised at a capture zone remote from the contact end;
ii) a plurality of capture probes, each capture probe being capable of hybridising to a different region of the target nucleic acid and each capture probe capable of being bound by the capture moiety when the capture probe has hybridised to the target nucleic acid; and
optionally iii) a detection probe capable of hybridising to the target nucleic acid and thereby allowing detection of the target nucleic acid utilising the detection probe, the detection probe being releasably immobilised to a probe zone of the chromatographic strip located between the contact end and the capture zone of the chromatographic strip, or the detection probe being separate from the dipstick.
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Abstract
Process for the production of methylenedianiline and its higher homologous products which comprises reacting aniline, or one of its derivatives, and formaldehyde, or one of its precursors, in one or two reactors in the presence of a solid acid catalyst selected from a zeolite or a silico-alumina, distilling the reaction water or the water added with the reagents.
36 Citations
47 Claims
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1. A kit for testing for the presence of target nucleic acid in a sample solution which comprises:
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i) a dipstick comprising;
a chromatographic strip having a contact end for contacting the sample solution;
a capture moiety, immobilised at a capture zone remote from the contact end;
ii) a plurality of capture probes, each capture probe being capable of hybridising to a different region of the target nucleic acid and each capture probe capable of being bound by the capture moiety when the capture probe has hybridised to the target nucleic acid; and
optionallyiii) a detection probe capable of hybridising to the target nucleic acid and thereby allowing detection of the target nucleic acid utilising the detection probe, the detection probe being releasably immobilised to a probe zone of the chromatographic strip located between the contact end and the capture zone of the chromatographic strip, or the detection probe being separate from the dipstick. - View Dependent Claims (3, 4, 5, 6, 7, 8, 21, 26, 27)
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2. A dipstick for testing for the presence of target nucleic acid in a sample solution which comprises:
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a chromatographic strip having a contact end for contacting the sample solution;
a capture moiety, immobilised at a capture zone remote from the contact end; and
a plurality of capture probes releasably immobilised at a probe zone of the chromatographic strip located between the contact end and the capture zone, each capture probe being capable of hybridising to a different region of the target nucleic acid and each capture probe capable of being bound by the capture moiety when the capture probe has hybridised to the target nucleic acid.
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9. A kit for testing for the presence of target nucleic acid in a sample solution which comprises:
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i) a dipstick comprising;
a chromatographic strip having a contact end for contacting the sample solution; and
a capture moiety immobilised at a capture zone remote from the contact end;
ii) a capture probe capable of hybridising to the target nucleic acid, wherein the capture probe comprises a plurality of capture ligands each of which can be bound by the capture moiety when the capture probe has hybridised to the target nucleic acid; and
optionallyiii) a detection probe capable of hybridising to the target nucleic acid and thereby allowing detection of the target nucleic acid utilising the detection probe, the detection probe being releasably immobilised to a probe zone of the chromatographic strip located between the contact end and the capture zone of the chromatographic strip, or the detection probe being separate from the dipstick.
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10. A dipstick for testing for the presence of target nucleic acid in a sample solution which comprises:
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a chromatographic strip having a contact end for contacting the sample solution;
a capture moiety immobilised at a capture zone remote from the contact end; and
a capture probe releasably immobilised to a probe zone of the chromatographic strip located between the contact end and the capture zone, the capture probe being capable of hybridising to the target nucleic acid, wherein the capture probe comprises a plurality of capture ligands each of which can be bound by the capture moiety when the capture probe has hybridised to the target nucleic acid. - View Dependent Claims (11, 12, 13, 14, 24, 25)
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15. A kit for testing for the presence of target nucleic acid in a sample solution which comprises:
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i) a dipstick comprising;
a chromatographic strip having a contact end for contacting the sample solution; and
a capture moiety immobilised at a capture zone remote from the contact end the capture moiety being capable of binding directly or indirectly to the target nucleic acid; and
ii) a plurality of detection probes, each detection probe being capable of hybridising to a different region of the target nucleic acid and thereby allowing detection of the target nucleic acid utilising the detection probes. - View Dependent Claims (17, 18, 22, 23, 38)
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16. A dipstick for testing for the presence of target nucleic acid in a sample solution which comprises:
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a chromatographic strip having a contact end for contacting the sample solution;
a capture moiety immobilised at a capture zone remote from the contact end, the capture moiety being capable of binding directly or indirectly to the target nucleic acid; and
a plurality of detection probes releasably immobilised at a probe zone of the chromatographic strip located between the contact end and the capture zone, each detection probe being capable of hybridising to a different region of the target nucleic acid and thereby allowing detection of the target nucleic acid utilising the detection probes. - View Dependent Claims (39)
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19. A kit for testing for the presence of target nucleic acid in a sample solution which comprises:
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i) a dipstick comprising;
a chromatographic strip having a contact end for contacting the sample solution;
a capture moiety immobilised at a capture zone remote from the contact end, the capture moiety being capable of binding directly or indirectly to the target nucleic acid; and
ii) a detection probe capable of hybridising to the target nucleic acid, wherein the detection probe comprises a plurality of labels allowing direct detection of the target nucleic acid utilising the detection probe, or wherein the detection probe comprises a plurality of detection ligands allowing indirect detection of the target nucleic acid utilising the detection probe. - View Dependent Claims (40)
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20. A dipstick for testing for the presence of target nucleic acid in a sample solution which comprises:
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a chromatographic strip having a contact end for contacting the sample solution;
a capture moiety immobilised at a capture zone remote from the contact end, the capture moiety being capable of binding directly or indirectly to the target nucleic acid; and
a detection probe releasably immobilised at a probe zone of the chromatographic strip located between the contact end and the capture zone, the detection probe being capable of hybridising to the target nucleic acid, wherein the detection probe comprises a plurality of labels allowing direct detection of the target nucleic acid utilising the detection probe, or wherein the detection probe comprises a plurality of detection ligands allowing indirect detection of the target nucleic acid utilising the detection probe.
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- 28. A probe for detecting or capturing target nucleic acid which comprises a nucleic acid or nucleic acid analogue capable of hybridising to the target nucleic acid, wherein the nucleic acid or nucleic acid analogue is coupled to a plurality of labels allowing direct detection of the target nucleic acid when the probe has hybridised to the target nucleic acid, or wherein the nucleic acid is coupled to a plurality of ligands which can be bound by a ligand binding moiety to detect or capture the target nucleic acid when the probe has hybridised to the target nucleic acid.
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36. Use of a plurality of different capture probes in a dipstick assay for testing for the presence of a target nucleic acid in a sample solution, each capture probe being capable of hybridising to a different region of the target nucleic acid, thereby allowing capture of the target nucleic acid to the dipstick by a capture moiety;
- immobilised to the dipstick, capable of binding the capture probes.
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37. Use of a plurality of different detection probes in a dipstick assay for testing for the presence of a target nucleic acid in a sample solution, each detection probe being capable of hybridising to a different region of the target nucleic acid, thereby allowing detection of the target nucleic acid utilising the detection probe.
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41. A method for testing for the presence of a target nucleic acid in a sample solution which comprises:
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a) providing a chromatographic strip having a contact end for contacting the sample solution and a capture moiety immobilised at a capture zone of the chromatographic strip remote from the contact end, the capture moiety being capable of binding directly or indirectly to the target nucleic acid;
b) contacting the contact end of the chromatographic strip with the sample solution to cause sample solution to move by capillary action to the capture zone so that target nucleic acid in the sample solution can be captured at the capture zone by the capture moiety;
(c) either;
(i) contacting the capture zone with a plurality of detection probes, each detection probe being capable of hybridising to a different region of the target nucleic acid and thereby allowing detection of the target nucleic acid utilising the detection probes;
or (ii) contacting the capture zone with a detection probe capable of hybridising to the target nucleic acid thereby allowing detection of the target nucleic acid utilising the detection probe, wherein the detection probe comprises a plurality of labels allowing direct detection of the target nucleic acid utilising the detection probe, or wherein the detection probe comprises a plurality of detection ligands allowing indirect detection of the target nucleic acid utilising the detection probe;
d) removing unbound detection probe from the capture zone of the chromatographic strip; and
e) detecting for the presence of the or each detection probe at the capture zone.
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42. A method for testing for the presence of target nucleic acid in a sample solution which comprises:
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providing a dipstick comprising a chromatographic strip having a contact end for contacting the sample solution and a capture moiety immobilised at a capture zone of the chromatographic strip remote from the contact end;
contacting the sample solution with a plurality of capture probes, each capture probe being capable of hybridising to a different region of the target nucleic acid and each capture probe capable of being bound by the capture moiety when the capture probe has hybridised to the target nucleic acid;
contacting the sample solution with the contact end of the chromatographic strip to cause sample solution to move by capillary action to the capture zone, thereby allowing a complex comprising target nucleic acid and the capture probes to be captured at the capture zone by binding of the capture moiety to the capture probes; and
detecting for target nucleic acid at the capture zone.
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43. A method for testing for the presence of target nucleic acid in a sample solution which comprises:
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providing a dipstick comprising a chromatographic strip having a contact end for contacting the sample solution and a capture moiety immobilised at a capture zone of the chromatographic strip remote from the contact end;
contacting the sample solution with a capture probe capable of hybridising to the target nucleic acid, wherein the capture probe comprises a plurality of capture ligands, each of which can be bound by the capture moiety when the capture probe has hybridised to the target nucleic acid;
contacting the sample solution with the contact end of the chromatographic strip to cause sample solution to move by capillary action to the capture zone, thereby allowing a complex comprising target nucleic acid and capture probe to be captured at the capture zone by binding of the capture moiety to the capture probe; and
detecting for target nucleic acid at the capture zone.
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44. A method for testing for the presence of target nucleic acid in a sample solution which comprises:
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providing a dipstick comprising a chromatographic strip having;
a contact end for contacting the sample solution;
a capture moiety immobilised at a capture zone of the chromatographic strip remote from the contact end; and
a plurality of capture probes releasably immobilised to the chromatographic strip between the contact end and the capture zone, each capture probe being capable of hybridising to a different region of the target nucleic acid and each capture probe capable of being bound by the capture moiety when the capture probe has hybridised to the target nucleic acid;
contacting the sample solution with the contact end of the chromatographic strip to cause sample solution to move by capillary action to the capture zone, thereby releasing the capture probes from the chromatographic strip so that they can hybridise to target nucleic acid in the sample solution as it moves to the capture zone and allowing a complex comprising target nucleic acid and the capture probes to be captured at the capture zone by binding of the capture moiety to the capture probes; and
detecting for target nucleic acid at the capture zone.
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45. A method for testing for the presence of target nucleic acid in a sample solution which comprises:
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providing a dipstick comprising a chromatographic strip having;
a contact end for contacting the sample solution;
a capture moiety immobilised at a capture zone of the chromatographic strip remote from the contact end; and
a capture probe capable of hybridising to the target nucleic acid, the capture probe being releasably immobilised to the chromatographic strip between the contact end and the capture zone, wherein the capture probe comprises a plurality of capture ligands, each of which can be bound by the capture moiety when the capture probe has hybridised to the target nucleic acid;
contacting the sample solution with the contact end of the chromatographic strip to cause sample solution to move by capillary action to the capture zone, thereby releasing the capture probe from the chromatographic strip so that it can hybridise to target nucleic acid in the sample solution as the sample solution moves to the capture zone and allowing a complex comprising target nucleic acid and the capture probe to be captured at the capture zone by binding of the capture moiety to the capture probe; and
detecting for target nucleic acid at the capture zone.
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46. A method for testing for the presence of target nucleic acid in a sample solution which comprises:
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providing a dipstick comprising a chromatographic strip having a contact end for contacting the sample solution and a capture moiety immobilised at a capture zone of the chromatographic strip remote from the contact end;
providing a plurality of capture probes, each capture probe being capable of binding to the capture moiety and hybridising to a different region of the target nucleic acid;
binding the plurality of capture probes to the capture moiety;
contacting the sample solution with the contact end of the chromatographic strip to cause sample solution to move by capillary action to the capture zone, thereby allowing target nucleic acid in the sample solution to be captured at the capture zone by binding of the target nucleic acid to the plurality of capture probes; and
detecting for target nucleic acid at the capture zone.
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47. A method for testing for the presence of target nucleic acid in a sample solution which comprises:
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providing a dipstick comprising a chromatographic strip having a contact end for contacting the sample solution and a capture moiety immobilised at a capture zone of the chromatographic strip remote from the contact end;
providing a plurality of capture probes, each capture probe being capable of binding to the capture moiety and hybridising to a different region of the target nucleic acid;
binding the plurality of capture probes to the capture moiety;
contacting the sample solution with the contact end of the chromatographic strip to cause sample solution to move by capillary action to the capture zone, thereby allowing target nucleic acid in the sample solution to be captured at the capture zone by binding of the target nucleic acid to the plurality of capture probes; and
detecting for target nucleic acid at the capture zone.
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Specification