Method for haplotyping by mass spectrometry

• US 20040053260A1
• Filed: 06/09/2003
• Published: 03/18/2004
• Est. Priority Date: 07/24/2000
• Status: Abandoned Application

• Current Assignee: Consortium National De Recherche En Genomique (CNRG)
• Original Assignee: Consortium National De Recherche En Genomique (CNRG)
• Inventors: Gut, Glynne Ivo, Lechner, Doris
• Application Number:
  US10/333,790
• Publication Number:
  US 20040053260A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2563/167 Mass label

Abstract

The invention relates to a method for performing haplotyping of multiple single nucleotide polymorphisms (SNPs) that uses allele specific PCR and mass spectrometry analysis.

Claims

1. A method for the determination of the haplotype of an individual, comprising the steps of:

d) genotyping of more than two single nucleotide polymorphisms (SNPs) by mass spectrometry;

e) allele specific PCR with one primer being specific for one allele of a heterozygous polymorphism, if more then one polymorphisms is heterozygous;

f) genotyping on the allele specific PCR product by mass spectrometry.

2. The method of claim 1, wherein the genotyping of the SNPs in step a), step c) or both steps is performed after generation of allele specific products, said generation of allele specific products being done by primer extension, oligonucleotide ligation, or a cleavase reaction.

3. The method of claim 1 or 2, wherein the genotyping for multiple SNPs in step a), step c) or both steps is performed in one reaction in a multiplexed procedure.

4. The method of any of claims 1 to 3, wherein the allele specific PCR reaction in step b) is achieved by choosing one primer that matches one allele of a heterozygous SNP.

5. The method of claim 4, wherein said primer is chosen as to specifically hybridize with the heterozygous SNP located at the most 5′

or the most 3′

location of all tested SNPs.

6. The method of any of claims 1 to 5, wherein at least one primer used for the allele specific PCR is fully complementary to the sequence of one allele.

7. The method of any of claims 1 to 6, wherein the 3′

end base of the allele specific primer specifically matches one allele of the heterozygous SNP.

8. The method of any of claims 6 to 7 in which said allele specific primer has 10 to 25 bases that are complementary to the sequence of said one allele of the genomic DNA.

9. The method of any of claims 4, 5, 7 and 8, wherein said allele specific primer has a 5'"'"'tail that is rich in G and C.

10. The method of any of claims 4, 5, 7 to 9 wherein said allele specific primer has one mismatch in the complementary sequence more then 3 bases away from the 3'"'"'end.

11. The method of any of claims 1 to 10 wherein matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is used for either of or both steps a) and c) as defined in claim 1.

12. The method of any of claims 1 to 11 wherein the primers used for generation of the products detected in the genotyping in steps a) and/or c) are chimeric in nature.

13. The method of claim 12, wherein the GOOD assay is applied for either of or both the genotyping steps.

14. The method of any of claims 1 to 13 wherein electrospray ionization mass spectrometry is used for either or both steps a) and c) of claim 1.

15. Kit for the implementation of haplotyping by the method according any of claims 1 to 14 comprising primers for PCR that generate allele specific products.