Nucleic-acid ink compositions for arraying onto a solid support
First Claim
1. A medium for suspending a solution of nucleic acid, the medium having a composition comprising:
- about 30% to about 80% by volume of an organic solution comprising dimethylsulfoxide (DMSO), ethylene glycol (EG), formamide, or a combination thereof, a buffer with a pH value of about 3.5-9.5;
water; and
nucleic acid, wherein the nucleic acid denatures to provide for more favorable hybridization.
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Abstract
A medium or ink solution containing nucleic acid is provided for depositing onto a solid support in the manufacture of biological arrays. The medium has a composition that comprises: about 30% to about 80% by volume of an organic solution comprising dimethylsulfoxide (DMSO), ethylene glycol (EG), formamide, or a combination thereof; a buffer with a pH value of about 3.5-9.5; water; and nucleic acid, wherein the nucleic acid denatures to provide for more favorable hybridization. The buffer can be made from a solution that may contain acetate, citrate, citrate-phosphate, maleate, or succinate. The medium permits long-term storage of nucleic acids in solution without excessive degradation, which is a phenomenon associated with many conventional ink solutions.
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Citations
80 Claims
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1. A medium for suspending a solution of nucleic acid, the medium having a composition comprising:
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about 30% to about 80% by volume of an organic solution comprising dimethylsulfoxide (DMSO), ethylene glycol (EG), formamide, or a combination thereof, a buffer with a pH value of about 3.5-9.5;
water; and
nucleic acid, wherein the nucleic acid denatures to provide for more favorable hybridization. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A medium for suspending a solution of nucleic acid, the medium having a composition comprising:
- a mixed organic solution of about 1% to about 55% by volume of ethylene glycol (EG) or formamide either individually, together, or with DMSO;
a buffer with a pH value of about 3.5-9.5;
water; and
nucleic acid. - View Dependent Claims (32, 33, 34, 35, 36, 37)
- a mixed organic solution of about 1% to about 55% by volume of ethylene glycol (EG) or formamide either individually, together, or with DMSO;
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38. A method for depositing a nucleic acid on a support, said method comprising:
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a) providing a solution of nucleic acid comprising about 30% to about 80% by volume of dimethylsulfoxide (DMSO), ethylene glycol (EG), formamide, or a combination thereof;
a buffer with a pH value of about 3.5-9.5;
water; and
a nucleic acid; and
b) depositing said solution on the support. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70)
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71. A method for depositing a nucleic acid on a solid support, said method comprising:
- depositing on the solid support a solution of nucleic acid comprising a mixed organic solution of about 1% to about 55% by volume of ethylene glycol (EG) or formamide either individually, together, or with DMSO;
a buffer with a pH value of about 3.5-9.5;
water; and
nucleic acid. - View Dependent Claims (72, 73, 74, 75, 76, 77, 78, 79, 80)
- depositing on the solid support a solution of nucleic acid comprising a mixed organic solution of about 1% to about 55% by volume of ethylene glycol (EG) or formamide either individually, together, or with DMSO;
Specification