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Methods for immobilizing polypeptides

  • US 20040058390A1
  • Filed: 09/23/2003
  • Published: 03/25/2004
  • Est. Priority Date: 06/19/2000
  • Status: Abandoned Application
First Claim
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1. A method for immobilizing a polypeptide to a surface, wherein the method comprises:

  • contacting a polypeptide which comprises an ester or thioester, with an anchor molecule comprising a first nucleophilic group at a 2 or 3 position relative to a second nucleophilic group, wherein the ester or thioester undergoes a trans-esterification reaction with the first nucleophilic group, thus forming an intermediate compound in which the polypeptide is attached to the anchor molecule through the first nucleophilic group; and

    attaching the anchor molecule to a surface.

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