Reactor systems having a light-interacting component

• US 20040058407A1
• Filed: 06/05/2003
• Published: 03/25/2004
• Est. Priority Date: 04/10/2001
• Status: Abandoned Application

• Current Assignee: BioProcessors Corp.
• Original Assignee: BioProcessors Corp.
• Inventors: Zarur, Andrey J., Miller, Scott E., Rodgers, Seth T., LeBlanc, Sean J.
• Application Number:
  US10/457,015
• Publication Number:
  US 20040058407A1
• Time in Patent Office: Days
• US Class Current: 435/29
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2400/046 Chemical or electrochemical formation of bubbles
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells

Abstract

Various aspects of the present invention relate to light-interacting components suitable for use in chips and other reactor systems. These components may include waveguides, optical fibers, light sources, photodetectors, optical elements, and the like. If waveguides are used, they may be fashioned out of any material able to transmit light to or from the reaction site. The chip may contain a reaction site having a volume of less than about 1 ml. In some embodiments, the chip may be constructed in such a way as to be able to support a living cell. The chip may be used for imaging or analysis, or the chip may be used to facilitate a chemical or biological reaction, which may be light-sensitive or light-activated in certain cases. Other facilitated reactions may include the production or consumption of a chemical or biological species. In some embodiments, the chip may include more than one component or component type, or more than one reaction site.

Claims

1. An apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

at least one waveguide in optical communication with the predetermined reaction site.

2. The apparatus of claim 1, comprising a plurality of reaction sites.

3. The apparatus of claim 1, wherein the predetermined reaction site has a volume of less than about 500 microliters.

4. The apparatus of claim 1, wherein the predetermined reaction site has a volume of less than about 100 microliters.

5. The apparatus of claim 1, wherein the predetermined reaction site has a volume of less than about 10 microliters.

6. The apparatus of claim 1, wherein the predetermined reaction site has a volume of less than about 1 microliter.

7. The apparatus of claim 1, wherein the predetermined reaction site has a maximum dimension of less than about 1 cm.

8. The apparatus of claim 1, wherein the predetermined reaction site has a maximum dimension of less than about 1 mm.

9. The apparatus of claim 1, wherein the predetermined reaction site has a maximum dimension of less than about 100 micrometers.

10. The apparatus of claim 1, wherein the predetermined reaction site has a maximum dimension of less than about 10 micrometers.

11. The apparatus of claim 1, wherein the waveguide is able to transmit light having a frequency of between about 350 nm and about 1000 nm.

12. The apparatus of claim 1, wherein the waveguide is able to transmit visible light.

13. The apparatus of claim 1, wherein the waveguide comprises a silicon-based material.

14. The apparatus of claim 13, wherein the silicon-based material comprises glass.

15. The apparatus of claim 13, wherein the silicon-based material comprises polysilicon.

16. The apparatus of claim 13, wherein the silicon-based material comprises quartz.

17. The apparatus of claim 13, wherein the silicon-based material comprises a carbide.

18. The apparatus of claim 13, wherein the silicon-based material comprises a nitride.

19. The apparatus of claim 13, wherein the silicon-based material comprises an oxide.

20. The apparatus of claim 1, wherein the chip comprises an inorganic material.

21. The apparatus of claim 20, wherein the inorganic material comprises a semiconductor.

22. The apparatus of claim 20, wherein the inorganic material comprises a metal.

23. The apparatus of claim 1, wherein the living cell is a mammalian cell.

24. The apparatus of claim 1, wherein the living cell is a bacterium.

25. The apparatus of claim 1, wherein the living cell is part of a tissue culture.

26. The apparatus of claim 1, wherein at least one surface of the predetermined reaction site comprises a polymer.

27. The apparatus of claim 26, wherein the at least one surface consists essentially of the polymer.

28. The apparatus of claim 26, wherein the polymer is selected from the group consisting of a silicone, a polycarbonate, a polyethylene, a polypropylene, a polytetrafluoroethylene, a apolyvinylidene chloride, a bis-benzocyclobutene, a polystyrene, a polyacrylate, a polymethacrylate, a polyimide, and combinations thereof.

29. The apparatus of claim 26, wherein the polymer is fluorinated.

30. The apparatus of claim 1, wherein the waveguide comprises a polymer.

31. The apparatus of claim 30, wherein the waveguide consists essentially of the polymer.

32. The apparatus of claim 30, wherein the waveguide comprises polystyrene.

33. The apparatus of claim 30, wherein the waveguide comprises polyacrylate.

34. The apparatus of claim 30, wherein the waveguide comprises polymethacrylate.

35. The apparatus of claim 30, wherein the waveguide comprises polycarbonate.

36. The apparatus of claim 30, wherein the waveguide comprises polyimide.

37. The apparatus of claim 30, wherein the waveguide comprises polyvinylidene fluoride.

38. The apparatus of claim 30, wherein the waveguide comprises polyethylene.

39. The apparatus of claim 30, wherein the waveguide comprises polypropylene.

40. The apparatus of claim 30, wherein the polymer is fluorinated.

41. The apparatus of claim 30, wherein the polymer is a copolymer.

42. A apparatus, comprising:

a chip comprising a predetermined reaction site having at least one substantially hydrophobic surface and a volume of less than about 1 ml; and

at least one waveguide in optical communication with the predetermined reaction site.

43. A apparatus, comprising:

a chip comprising a predetermined reaction site having at least one substantially hydrophilic surface and a volume of less than about 1 ml; and

at least one waveguide in optical communication with the predetermined reaction site.

44. A apparatus, comprising:

a chip comprising a predetermined reaction site having at least one substantially cytophilic surface and a volume of less than about 1 ml; and

at least one waveguide in optical communication with the predetermined reaction site.

45. A apparatus, comprising:

a chip comprising a predetermined reaction site having at least one substantially cytophobic surface and a volume of less than about 1 ml; and

at least one waveguide in optical communication with the predetermined reaction site.

46. A apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml; and

a milled waveguide in optical communication with the predetermined reaction site.

47. A apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml; and

a machined waveguide in optical communication with the predetermined reaction site.

48. A apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

an optical element in optical communication with the predetermined reaction site.

49. The apparatus of claim 48, wherein the optical element is integrally connected to the apparatus.

50. The apparatus of claim 48, wherein optical element is a diffraction grating.

51. The apparatus of claim 48, wherein the optical element is a lens.

52. The apparatus of claim 51, wherein the lens is a diverging lens.

53. The apparatus of claim 51, wherein the lens comprises a graded index material.

54. The apparatus of claim 48, wherein the optical element is constructed and arranged to focus light on a waveguide.

55. The apparatus of claim 48, wherein the optical element is constructed and arranged to focus light on a point located within the predetermined reaction site.

56. The apparatus of claim 48, wherein the optical element is positioned so as to be able to focus light that will enter the predetermined reaction site.

57. The apparatus of claim 48, wherein the optical element is positioned so as to able to collect light emitted from a point located within the predetermined reaction site.

58. A apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

a photodetector in optical communication with the predetermined reaction site.

59. The apparatus of claim 58, wherein the photodetector is able to detect the presence of a cell at the predetermined reaction site.

60. The apparatus of claim 59, wherein the cell is a mammalian cell.

61. The apparatus of claim 59, wherein the photodetector is able to detect adhesion of the cell at the predetermined reaction site.

62. The apparatus of claim 59, wherein the photodetector is able to detect a location of the cell at the predetermined reaction site.

63. The apparatus of claim 58, wherein the photodetector is in optical communication with a waveguide.

64. The apparatus of claim 58, wherein the photodetector is able to detect light having a frequency of between about 350 nm and about 1000 nm.

65. The apparatus of claim 58, wherein the photodetector comprises a photomultiplier.

66. The apparatus of claim 58, wherein the photodetector comprises a photodiode.

67. A method, comprising:

providing a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site;

providing material in the predetermined reaction site, the material having a smallest dimension;

directing electromagnetic radiation having an average beam diameter less than the smallest dimension of the material;

allowing the electromagnetic radiation to interact with the material to produce altered radiation; and

determining the altered radiation.

68. The method of claim 67, wherein the determining step comprises quantifying the altered radiation.

69. The method of claim 67, wherein the electromagnetic radiation comprises visible light.

70. The method of claim 69, wherein the electromagnetic radiation consists essentially of visible light.

71. The method of claim 67, further comprising the step of determining a property of the interaction based on the measuring step.

72. The method of claim 67, wherein the measuring step comprises determining the optical density of the altered light.

73. The method of claim 67, wherein the interaction comprises fluorescence.

74. The method of claim 67, wherein the interaction comprises light scattering.

75. The method of claim 67, wherein the electromagnetic radiation is substantially monochromatic.

76. The method of claim 67, wherein the material comprises a cell.

77. The method of claim 67, wherein the electromagnetic radiation has a wavelength of between about 350 nm and about 1000 nm.

78. A method, comprising:

providing a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

optically causing a biological change in a biological material located at the predetermined reaction site.

79. The method of claim 78, wherein the biological material comprises a cell.

80. The method of claim 79, wherein the causing step comprises causing the cell to photosynthesize.

81. The method of claim 79, wherein the causing step comprises killing the cell.

82. The method of claim 79, wherein the cell is a plant cell.

83. A apparatus, comprising:

a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

a source of light in optical communication and integrally connected with the predetermined reaction site.

84. The apparatus of claim 83, wherein the source of light comprises a light-emitting diode.

85. The apparatus of claim 83, wherein the source of light comprises a laser.

86. The apparatus of claim 85, wherein the laser comprises a semiconductor laser.

87. The apparatus of claim 85, wherein the laser comprises a quantum well laser.

88. A method, comprising:

providing a chip having a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site;

providing material in the predetermined reaction site;

directing light from a source within the chip at the material; and

producing an image of the material.

89. A apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

a filter able to filter light entering or exiting the predetermined reaction site, wherein the filter is integrally connected to the chip.

90. A apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

a light-interacting component integrally connected to the predetermined reaction site.

91. A apparatus, comprising:

a chip comprising a predetermined reaction site having a volume of less than about 1 ml; and

an actuator able to target a first cell type within the predetermined reaction site without targeting a second cell type.

92. An apparatus, comprising:

a chip comprising a predetermined reaction site having an inlet, an outlet, and a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site, wherein the chip is substantially transparent.

93. A method, comprising:

providing a chip comprising a predetermined reaction site having an inlet, an outlet, and a volume of less than about 1 ml, the predetermined reaction site constructed and arranged to maintain at least one living cell at the site; and

optically addressing the predetermined reaction site.

94. The method of claim 93, wherein optically addressing comprises optically addressing the predetermined reaction site using light that is in line-of-sight optical communication with the predetermined reaction site.

95. The method of claim 93, wherein optically addressing comprises optically addressing the predetermined reaction site using substantially monochromatic light.

96. The method of claim 93, wherein optically addressing comprises causing a light-sensitive or a light-activated reaction to occur.