Electroprocessing in drug delivery and cell encapsulation

• US 20040058887A1
• Filed: 09/22/2003
• Published: 03/25/2004
• Est. Priority Date: 08/31/1999
• Status: Active Grant

• Current Assignee: Virginia Commonwealth University
• Original Assignee: Virginia Commonwealth University
• Inventors: Bowlin, Gary L., Simpson, David G., Wnek, Gary E.
• Granted Patent: US 7,374,774 B2
• Time in Patent Office: Days
• US Class Current: 514/44
• CPC Class Codes: A61F 2/08 Muscles; Tendons; Ligaments sutures A61B17/04
• CPC Class Codes: A61K 2035/126 Immunoprotecting barriers, e.g. jackets, diffusion chambers
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 15/32 Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin A61L15/225 takes precedence
• CPC Class Codes: A61L 2400/04 Materials for stopping bleeding
• CPC Class Codes: A61L 2430/30 for muscle reconstruction
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/225 Fibrin; Fibrinogen
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3847 Bones
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 31/041 Mixtures of macromolecular compounds
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: B29C 41/006 using an electrostatic field for applying the material
• CPC Class Codes: B29C 67/0007 : Manufacturing coloured articles not otherwise provided for, e.g. by colour change
• CPC Class Codes: C07K 14/75 : Fibrinogen
• CPC Class Codes: C07K 14/78 : Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C08L 67/04 : Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 89/00 : Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C08L 89/06 : derived from leather or skin , e.g. gelatin
• CPC Class Codes: C12N 2503/00 : Use of cells in diagnostics
• CPC Class Codes: C12N 2510/00 : Genetically modified cells
• CPC Class Codes: C12N 2533/40 : Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2533/54 : Collagen; Gelatin
• CPC Class Codes: C12N 2533/56 : Fibrin; Thrombin
• CPC Class Codes: C12N 5/0012 : Cell encapsulation
• CPC Class Codes: C12N 5/0062 : General methods for three-dimensional culture
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0622 : Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells

Abstract

The invention is directed to novel compositions comprising an electroprocessed material and a substance, their formation and use. The electroprocessed material can, for example, be one or more natural materials, one or more synthetic materials, or a combination thereof. The substance can be one or more therapeutic or cosmetic substances or other compounds, molecules, cells, vesicles. The compositions can be used in substance delivery, including drug delivery within an organism by, for example, releasing substances or containing cells that release substances. The compositions can be used for other purposes, such as prostheses or similar implants.

Claims

1. A composition comprising:

an electroprocessed material and a substance.

2. The composition of claim 1, wherein the material is one or more natural materials, one or more synthetic materials, or a combination thereof.

3. The composition of claim 1, wherein the material is a combination of one or more natural materials and one or more synthetic materials.

4. The composition of claim 2, wherein the natural material comprises one or more amino acids, peptides, denatured peptides, polypeptides, proteins, carbohydrates, lipids, nucleic acids, glycoproteins, lipoproteins, glycolipids, glycosaminoglycans, proteoglycans, or a combination thereof.

5. The composition of claim 2, wherein the synthetic material comprises one or more polymers.

6. The composition of claim 1, wherein the substance comprises a therapeutic substance, a cosmetic substance or a combination thereof

7. The composition of claim 1, wherein the substance comprises one or more molecules, cells, objects, or combinations thereof.

8. The composition of claim 1, further comprising placement of the composition in a bioreactor.

9. The composition of claim 1, wherein the electroprocessed material is crosslinked.

11. A method of delivering a substance to a desired location, comprising placing the composition of claim 1 at the desired location.

13. The method of claim 11, wherein the substance comprises a molecule or a cell that will release the molecule after placement in the desired location.

14. The method of claim 11, wherein the substance comprises a therapeutic substance, a cosmetic substance or a combination thereof.

15. The method of claim 11, wherein the substance comprises one or more molecules, cells, objects, or combinations thereof.

16. The method of claim 11, wherein the substance comprises a cell.

17. The method of claim 11, wherein the location is inside or upon the body of a human or an animal.

18. A method of manufacturing the composition of claim 1, comprising:

electrodepositing one or more electrically-charged solutions comprising a material or molecules capable of forming the material onto a grounded substrate under conditions effective to electrodeposit the material or the molecules capable of forming the material on the substrate to form the electroprocessed material; and

, adding the substance to the electrodeposited material to form the composition.

19. The method of claim 18, wherein the material is one or more natural materials, one or more synthetic materials, or a combination thereof.

20. The method of claim 18, wherein the substance comprises a therapeutic substance, a cosmetic substance or a combination thereof.

21. The method of claim 18, wherein the substance comprises one or more molecules, cells, objects, or combinations thereof.

10. A composition comprising an electroprocessed material, wherein the electroprocessed material provides a biological effect when delivered to a desired location in vivo or in vitro.

12. A method of delivering an electroprocessed material to a desired location, comprising placing the composition of claim 10 at the desired location.

22. A method of manufacturing the composition of claim 10, comprising electrodepositing one or more electrically-charged solutions comprising a material or molecules capable of forming the material onto a grounded substrate under conditions effective to electrodeposit the material or the molecules capable of forming the material on the substrate to form the electroprocessed material.

23. The method of claim 22, wherein the material is one or more natural materials, one or more synthetic materials, or a combination thereof.