Method and apparatus for detecting fluorescene of a sample
First Claim
1. An apparatus for detecting the fluorescence of a sample comprising a first excitation light means comprising at least a first light source, the first light source having a main light path, a sample plane for positioning said sample, first detection means comprising at least a first detector for detecting fluorescence signals from the sample, the axis between the detection means and the sample plane being the detection-sample axis, a processor coupled to receive data from the detector(s), a focusing means for focusing the signals or to the detection means, said focusing means having a collection angle, the angle between the excitation main light path of the from the first light source and the detection-sample axis is between the collection angle/2 and 90°
- , and wherein a second detection means is arranged so that the sample plane is positioned between the first detection means and the second detection means, and/or the excitation light is arranged as at least two light sources, and the excitation light filter(s) for each light source are connected to each other on a supporting material.
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Abstract
The present invention relates to an apparatus and a method for detecting the fluorescence of a sample, wherein the apparatus comprises excitation light source located on the same side of the sample as the detecting means. The apparatus may be constructed as a single-sided as well as a double-sided system, wherein the double-sided system comprises double-sided excitation system and/or double-sided detecting system. The double-sided system may also be a combination of fluorescence system and conventional microscopy. The apparatus may be used for analysis of various types of biological material, liquid as well as solid material, and various other types of material suitable being analysed through fluorescence studies.
58 Citations
54 Claims
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1. An apparatus for detecting the fluorescence of a sample comprising
a first excitation light means comprising at least a first light source, the first light source having a main light path, a sample plane for positioning said sample, first detection means comprising at least a first detector for detecting fluorescence signals from the sample, the axis between the detection means and the sample plane being the detection-sample axis, a processor coupled to receive data from the detector(s), a focusing means for focusing the signals or to the detection means, said focusing means having a collection angle, the angle between the excitation main light path of the from the first light source and the detection-sample axis is between the collection angle/2 and 90° - , and
wherein a second detection means is arranged so that the sample plane is positioned between the first detection means and the second detection means, and/or the excitation light is arranged as at least two light sources, and the excitation light filter(s) for each light source are connected to each other on a supporting material. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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2. An apparatus for detecting the fluorescence of a sample comprising
a first excitation light means comprising at least a first light source, the first light source having a main light path, a sample plane for positioning said sample, detection means comprising at least a first detector for detecting fluorescence signals from the sample, the axis between the detection means and the sample plane being the detection-sample axis, a processor coupled to receive data from the detector(s), a focusing means for focusing the signals or to the detection means, said focusing means having a collection angle, the angle between the excitation main light path of the from the first light source and the detection-sample axis is between the collection angle/2 and 90° - , and the focusing means provides a magnification in the range of 2/1 to 1/10.
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24. A method of assessing a parameter of a sample comprising
arranging the sample in a sample plane, exposing a first surface of the sample directly with excitation light from a first light means having at least a first light source, by use of focusing means detecting a fluorescence signal from the first surface of the sample onto a first detection means comprising at least a first detector, and onto a second detection means, wherein the second detection means is arranged so that the sample is positioned between the first detection means and the second detection means, processing the detected signal obtaining signal data, correlating the signal data to the parameter to be assessed, and assessing the parameter.
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25. A method of assessing a parameter of a sample comprising
arranging the sample in a sample plane, exposing a first surface of the sample directly with excitation light from a first light means having at least a first light source, by use of focusing means detecting a fluorescence signal from the first surface of the sample onto a first detection means comprising at least a first detector, the focusing means providing a magnification in the range of 2/1 to 1/10, processing the detected signal obtaining signal data, correlating the signal data to the parameter to be assessed, and assessing the parameter.
Specification