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Method and apparatus for detecting fluorescene of a sample

  • US 20040061070A1
  • Filed: 10/10/2002
  • Published: 04/01/2004
  • Est. Priority Date: 04/11/2000
  • Status: Active Grant
First Claim
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1. An apparatus for detecting the fluorescence of a sample comprising a first excitation light means comprising at least a first light source, the first light source having a main light path, a sample plane for positioning said sample, first detection means comprising at least a first detector for detecting fluorescence signals from the sample, the axis between the detection means and the sample plane being the detection-sample axis, a processor coupled to receive data from the detector(s), a focusing means for focusing the signals or to the detection means, said focusing means having a collection angle, the angle between the excitation main light path of the from the first light source and the detection-sample axis is between the collection angle/2 and 90°

  • , and wherein a second detection means is arranged so that the sample plane is positioned between the first detection means and the second detection means, and/or the excitation light is arranged as at least two light sources, and the excitation light filter(s) for each light source are connected to each other on a supporting material.

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