Hydrogel formulations for use in electroosmotic extraction and detection of glucose
First Claim
1. A hydrogel patch, comprising:
- (a) a hydrophilic compound which forms a gel in the presence of water, which compound is present in an amount of about 4% or more by weight based on the weight of the hydrogel;
(b) water in an amount of about 95% or less based on the weight of the hydrogel;
(c) an enzyme capable of catalyzing a reaction; and
(d) an electrolyte.
1 Assignment
0 Petitions
Accused Products
Abstract
A hydrogel patch is disclosed which is comprised of a polymeric material which forms a gel with water with the material being present in an amount of about 0.5% to 40% by weight based on the weight of the patch. Electrical conductively of the water is increased by the addition of an electrolyte. The patch comprises an enzyme which is capable of catalyzing a reaction with a biomedically important molecule such as glucose. Glucose drawn into the patch undergoes a reaction with the aid of the enzyme and the hydrogen peroxide released flows through the electrically conductivity of the water and may react at an electrode surface to generate a signal related to the amount of glucose entering the patch. The patch is also preferably comprised of a buffer which maintains the pH of the patch in the range of from about 3 to 9, and may be further comprised of a cross-linking agent, a biocide, a humectant, and a surfactant. The patch is preferably in the form of a thin (5 μm-50 mils), flat circular disc (0.5 to 10 cm2 of area) which will conform to the contours of human skin and may have a non-woven fabric embedded therein and removable release liners on each surface.
-
Citations
27 Claims
-
1. A hydrogel patch, comprising:
-
(a) a hydrophilic compound which forms a gel in the presence of water, which compound is present in an amount of about 4% or more by weight based on the weight of the hydrogel;
(b) water in an amount of about 95% or less based on the weight of the hydrogel;
(c) an enzyme capable of catalyzing a reaction; and
(d) an electrolyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
-
17. An absorbent material patch, characterized by:
-
(a) an absorbent material having embedded therein a dry enzyme;
(b) a package attached to a first surface of the absorbent material, the package containing an aqueous solution of water having dissolved therein an electrolyte, the package being separated from the absorbent material by a seal which is breakable on the application of force and further wherein the package is readily detachable from the absorbent material after the seal is broken. - View Dependent Claims (18, 19, 20, 21)
-
-
22. A patch having a thickness in a range of about 5 μ
- m to 50 mils and a first and a second surface each having an area in a range of about 0.5 cm2 to about 10 cm2, comprising;
a material which holds water in place;
an enzyme which catalyzes a reaction with glucose. - View Dependent Claims (23, 24, 25, 26)
- m to 50 mils and a first and a second surface each having an area in a range of about 0.5 cm2 to about 10 cm2, comprising;
-
27. A dry gel patch on a solid support, prepared by the method comprising:
-
(a) mixing dry gel components and an amount of water to form a gel mixture;
(b) cross-linking the gel mixture to form a hydrated gel;
(c) attaching the hydrated gel to a solid support; and
(d) drying the gel on the solid support, wherein said dry gel components comprise a hydrophilic compound which forms a gel in the presence of water, which compound is present in an amount of about 4% or more by weight based on the weight of the hydrated gel, an enzyme capable of catalyzing a reaction, an electrolyte, and wherein the amount of water is about 95% or less based on the weight of the hydrated gel.
-
Specification