Cell adhesion resisting surfaces
First Claim
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12. A process of coating a plasma-treated surface of an article, which surface comprises nitrogen-containing groups, with HA or AA or a derivative of HA or AA, said process comprising:
- exposing the plasma-treated surface to a solution of HA, AA or said derivative in the presence of a carbodiimide and a reactive intermediate stabilizing compound, to obtain a coated surface.
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Abstract
A coated surface that resists cell adhesion comprisesg hyaluronic acid directly bound to a plasma-treated polymer surface. A process for producing the coated surface is disclosed as are further modifications of the hyaluronic acid by attaching ligand-binding polypeptides (antibodies or antibody binding proteins).
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Citations
58 Claims
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12. A process of coating a plasma-treated surface of an article, which surface comprises nitrogen-containing groups, with HA or AA or a derivative of HA or AA, said process comprising:
exposing the plasma-treated surface to a solution of HA, AA or said derivative in the presence of a carbodiimide and a reactive intermediate stabilizing compound, to obtain a coated surface. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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13. A process of coating a plasma-treated surface of an article, which surface comprises nitrogen-containing groups, with HA or AA or a derivative of HA or AA, said process consisting essentially of:
exposing the plasma-treated surface to a solution of HA, AA or said derivative in the presence of a carbodiimide and a reactive intermediate stabilizing compound, to obtain a coated surface.
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20-1. The process of claim 12 wherein the ratio of carbodiimide- and ester stabilizing compound-to-HA repeat unit is at least 1 and 0.5.
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21-2. A surface that is formed by the process of claim 1.
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22-3. A surface that is formed by the process of claims 12.
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48. An article useful for binding a target cell or target molecule, which target cell or molecule comprises a target ligand, said article comprising;
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(a) a CAR surface coated with a layer comprising HA, AA, or a derivative of HA or AA;
(b) covalently bonded to said HA, AA or derivative, a first ligand-binding polypeptide that is capable of binding to said target ligand. - View Dependent Claims (49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
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Specification