Heparanase specific molecular probes and their use in research and medical applications

• US 20040063135A1
• Filed: 10/02/2003
• Published: 04/01/2004
• Est. Priority Date: 09/02/1997
• Status: Active Grant

• Current Assignee: Hadasit Medical Research Services & Development Ltd (Hadassah, The Women's Zionist Organization of America, Inc.), Insight Strategy & Marketing Ltd.
• Original Assignee: Hadasit Medical Research Services & Development Ltd (Hadassah, The Women's Zionist Organization of America, Inc.), Insight Strategy & Marketing Ltd.
• Inventors: Friedmann, Yael, Pecker, Iris, Peretz, Tuvia, Vlodavsky, Israel
• Granted Patent: US 7,339,038 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61M 15/009 using medicine packages with incorporated spraying means, e.g. aerosol cans pocket atomiser of the injector type A61M11/08
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C07K 16/40 against enzymes
• CPC Class Codes: C12N 9/2402 hydrolysing O- and S- glycosyl compounds (3.2.1)
• CPC Class Codes: C12Y 302/01166 Heparanase (3.2.1.166)
• CPC Class Codes: G01N 2333/988 Lyases (4.), e.g. aldolases, heparinase, enolases, fumarase
• CPC Class Codes: G01N 2400/40 Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
• CPC Class Codes: G01N 33/573 for enzymes or isoenzymes

Abstract

A variety of heparanase specific molecular probes which can be used for research and medical applications including diagnosis and therapy. Specific applications include the use of a heparanase specific molecular probe for detection of the presence, absence or level of heparanase expression; the use of a heparanase specific molecular probe for therapy of a condition associated with expression of heparanase; the use of a heparanase specific molecular probe for quantification of heparanase in a body fluid; the use of a heparanase specific molecular probe for targeted drug delivery; and the use of a heparanase specific molecular probe as a therapeutic agent.

Claims

1. An oligonucleotide comprising a nucleic acid sequence specifically hybridizable with heparanase encoding nucleic acid.

2. An antisense nucleic acid molecule comprising a nucleic acid sequence specifically hybridizable with heparanase messenger RNA.

3. A sense nucleic acid molecule comprising a nucleic acid sequence specifically hybridizable with heparanase antisense RNA.

4. A pair of polymerase chain reaction primers comprising a sense primer and an antisense primers, each of said primers including a nucleic acid sequence specifically hybridizable with heparanase encoding nucleic acid.