Method for detecting biomarkers

• US 20040063216A1
• Filed: 06/24/2003
• Published: 04/01/2004
• Est. Priority Date: 12/24/2000
• Status: Abandoned Application

• Current Assignee: Iser Lubocki
• Original Assignee: Iser Lubocki
• Inventors: Lubocki, Iser
• Application Number:
  US10/451,746
• Publication Number:
  US 20040063216A1
• Time in Patent Office: Days
• US Class Current: 436/173
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/4088 Diagnosing of monitoring cognitive diseases, e.g. Alzheimer, prion diseases or dementia
• CPC Class Codes: A61B 5/411 Detecting or monitoring allergy or intolerance reactions to an allergenic agent or substance
• CPC Class Codes: A61B 5/415 the glands, e.g. tonsils, adenoids or thymus
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

According to the present invention, there is provided a noninvasive method for rapid detection of biomarkers, including the steps of subjecting biological material to laser beam irradiation of a single wavelength, enhancing the scattered light returned from passage of said excitation light through the biological material, transmitting this scattered light and measuring it, to obtain data which is characteristic of the biological material being tested, comparing the spectral data to reference spectral data obtained from laser irradiation of the same wavelength applied to a known biological sample contaminated with the biomarker being detected, receiving a diagnosis of the presence or absence of biomarkers of the disease in the biological material, and determining the quantitative value of the biomarkers in the biological material. Also provided is a diagnostic tool for detecting the presence of a biomarker in a sample, the tool including a laser beam irradiation device with excitation light of a certain wavelength, an enhancing kit for enhancing the scattered light returned from passage of the excitation light, through the biological material, and a measurement device to obtain data which is characteristic of the biological material being tested, a comparing device for comparing the data to reference spectral data obtained from laser irradiation of the same wavelength applied to a known biological sample that has the biomarker, a quantitator for quantitating the value of the biomarker in the biological material and a display device for displaying the diagnosis.

Claims

1. A method for rapid detection of biomarkers of diseases, comprising the steps of:

subjecting biological material to laser beam irradiation of certain wavelength;

enhancing the scattered light returned from passage of said excitation light, through the biological material;

measuring the scattered light returned, to obtain data which is characteristic of the biological material being tested;

comparing the spectral data to reference spectral data obtained from laser irradiation of the same wavelength applied to a known similar biological material which is a carrier of the biomarker being detected;

receiving a diagnosis of the presence or absence of the biomarkers of the disease in the biological material; and

determining the quantitative value of the biomarkers of the disease in the biological material.

2. The method for rapid detection of biomarkers according to claim 1, wherein said enhancing step can be done before or after subjecting the biological material to a laser beam

3. The method for rapid detection of biomarkers according to claim 1, wherein said enhancing step includes subjecting the biological material or the light scattering to metal surfaced enhancement in a resonant and off-resonant enhancement.

4. The method for rapid detection of biomarkers according to claim 1, wherein the said transmitting step includes transmission of the biological material, or the spectral data obtained from the biological material for analysis at a remote physical location.

5. The method for rapid detection of biomarkers according to claim 1, further comprising measuring the plot of intensity of scattered light versus energy difference, returned after passage of laser light through said biological sample is performed using at least one selected from the group consisting of a spectrometer, a monochrometer, and an interferometer.

6. The method according to claim 1, wherein said method is used for measuring Transmissible Spongiform Encephalopathy in a sample.

7. A diagnostic tool for detecting the presence and quantitative value of a biomarker in a biological material, said tool comprising:

enhancing means for metal surfaced enhancement with resonant and off-resonant enhancement or with chromophore enhancement.

a laser beam irradiation device with excitation light of a certain wavelength;

transmitting means for transmitting the scattered light returned from passage of the excitation light through the biological material;

measuring means for measuring the wavelength to obtain data which is characteristic of the biological material being tested;

comparing means for comparing the data to reference spectral data obtained from laser irradiation of the same wavelength applied to a known similar biological sample that has the same biomarker;

in order to obtain a diagnosis of the presence or absence of the biomarkers of the disease in the biological material;

quantitative means for quantifying the value of the biomarker in the biological material.

8. The tool according to claim 7, further including a collecting and transporting device which is preferably a transparent kit with a rough surface or a special stone, in which the biological material is stored in a manner which enables it to be concentrated, preserved and transported.

9. The tool according to claim 7, wherein said laser device is selected from an adjustable laser titanium sapphire diode, a continuous wave laser diode, a neodymium doped yttrium aluminum gamet laser (Nd-YAG laser), an optical parametric oscillator, gas ion lasers, and solid state lasers.

10. The tool according to claim 7, wherein said laser beam has a wavelength within the range of 210 of 1500 nanometers.

11. The tool according to claim 7, wherein said enhancing means is a compound selected from the group consisting essentially of silver, gold, copper, other metals, chromophores such as:

metalloporphyrins, carotenoides, fullerenes, polydiacetylenes, fluorophores Hex and Rhodamine and combinations thereof, and is placed in close proximity to said biological material.

12. The tool according to claim 7, further comprising transmitting means for transmitting said data obtained from said biological material for analysis at a remote physical location, consisting of:

fiber-optics, Internet, wirelessly or radio waves.

13. The tool according to claim 7, wherein said measuring means is selected from the group consisting essentially of a spectrometer, a monochrometer, and an interferometer.

14. The tool according to claim 7, wherein said comparing means is a comparing device which is capable of comparing the data obtained from the measurement such as a computer.

15. The tool according to claim 7, wherein said quantitating mean is a foreign compound with a pre known Raman spectra and concentration which is used as a comparison baseline.

16. The tool according to claim 7, further comprising compiling and displaying means for compiling, displaying and delivering said data to an end user in a simple illustrative manner.

17. The tool according to claim 7, wherein said tool is a mobile hand-held device.

18. A method for detecting biomarkers, comprising the steps of:

subjecting biological material to laser beam irradiation of a certain wavelength and detecting at least one biomarker for a disease by obtaining data which is characteristic of the biomarker.