Method for producing arrays and devices relating thereto

• US 20040063221A1
• Filed: 06/13/2003
• Published: 04/01/2004
• Est. Priority Date: 10/16/1997
• Status: Abandoned Application

• Current Assignee: Larry S. Millstein
• Original Assignee: Larry S. Millstein
• Inventors: Millstein, Larry S.
• Application Number:
  US10/460,417
• Publication Number:
  US 20040063221A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00522 in a multiple parallel arrangement
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00664 Three-dimensional arrays
• CPC Class Codes: B01J 2219/00673 Slice arrays
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B26D 3/161 : for obtaining more than one product at a time
• CPC Class Codes: C03B 37/0756 : Hollow fibres
• CPC Class Codes: C03B 37/16 : Cutting or severing light guides G02B6/25
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 1/36 : Embedding or analogous mounting of samples
• CPC Class Codes: G01N 33/54313 : the carrier being characterised by its particulate form
• CPC Class Codes: Y10T 156/1062 : Prior to assembly

Abstract

A method of making replicate arrays, comprises repeatedly sectioning a bundle of aligned array members to make wafers comprising replicate arrays, wherein: each array comprises structural members each of which has a lumen therethrough which is continuously enclosed thereby; each array member is a homogenous composition disposed within a separate lumen of a structural member which extends from a first to a second wafer surface formed by said sectioning; and each structural member and each array member are aligned in the bundle parallel to an alignment axis and occupy a defined position in the two dimensions orthogonal thereto; wherein the array members comprise analyte binding reagents that hybridize to DNA or RNA having specific nucleotide sequences.

Claims

19. A method for making arrays, comprising the step of cross-sectioning a plurality of aligned array members comprising at least two array members different from one another.

20. A method for making replica arrays, comprising repeatedly cross-sectioning a plurality of aligned array members to produce sections with at least one surface that exposes array members in the same disposition, thereby replicating the array.

21. A method for making arrays for detecting a plurality of analytes, comprising the steps of:

(A) providing a plurality of analyte binding reagents array members;

(B) forming bundle members comprising of or comprising the array members;

(C) assembling the bundle members to form a bundle in which the array members are aligned;

(D) sectioning the bundle to produce wafers that comprise an array of the analyte binding reagents.

1. A method for making arrays of a plurality of array members, comprising the steps of:

(A) providing a plurality of array members;

(B) forming bundle members comprising the array members;

(C) assembling the bundle members to form a bundle in which the array members are aligned;

(D) sectioning the bundle to produce wafers that comprise an array of the array members.

2. A method according to claim 1, wherein the array members are cross-sectioned perpendicular to their alignment.

3. A method according to claim 1, wherein the array members are cross-sectioned at an angle of 10 to 80 degrees or 100 to 170 degrees to their alignment.

4. A method according to any of the foregoing claims, wherein the array members are cross-sectioned by a smooth planar cut.

5. A method according to any of claims 1 to 3, wherein the array members are cross-sectioned by a non-planar cut.

6. A method according to claim 5, wherein the surface area of array members exposed by cross-sectioning is increased over that provided by a smooth, planar cut.

7. A method according to claim 1, wherein airway members are comprised of or are disposed within a plastic, a glass, a metal or a ceramic.

8. A method according to claim 7, wherein array members are comprised of or disposed within a glass.

9. A method according to claim 7, wherein array members are comprised of or disposed within a plastic.

10. A method according to claim 9, wherein the plastic is a polycarbonate, polyethylene, polymethylmethacrylate, polystyrene, a copolymer of polystyrene, polysulfone, polyvinylchloride, polyester, polyamide, polyacetal, polyethyleneterephthalate, polytetrafluoroethylene or polyurethane.

11. A method according to claim 10, wherein the plastic is a polycarbonate, polyethylene, polystyrene, a copolymer of polystyrene, polysulfone or polyvinylchloride.

12. A method according to claim 1, wherein the array members are spaced about 1.0 to about 1,000 micrometers apart.

13. A method according to claim 1, wherein the array members have a cross-sectional area of about 1.0 to about 1,000,000 μ

m².

14. A method according to claim 1, wherein the density of array members in the array is about 250 to about 2,500,000 array members per square centimeter of cross sectional surface area of the array.

15. A method according to claim 1, wherein the density in the array is about 10 to about 100,000 array members per square centimeter of total surface area at the assay.

16. A method according to claim 1, wherein there are about 100 to about 2,500,000 aligned array members.

17. A method according to claim 1, wherein there are about 100 to 2,500,000 different aligned array members.

18. A method according to claim 1, wherein cross-sectioning produces sections about 2.5 to about 2,500 micrometers thick.

22. A method according to claim 21, wherein the array members are cross-sectioned perpendicular to their alignment.

23. A method according to claim 21, wherein the array members are cross-sectioned at an angle of 10-80 degrees or 100-170 degrees to their alignment.

24. A method according to any of claims 21 to 23, wherein the array members are cross-sectioned by a smooth planar cut.

25. A method according to any of claims 21 to 23, wherein the array members are cross-sectioned by a non-planar cut.

26. A method according to claim 25, wherein the surface area of array members exposed by cross-sectioning is increased over that provided by a smooth, planar cut.

27. A method according to claim 21, wherein array members are comprised of or are disposed within a plastic, a glass, a metal or a ceramic.

28. A method according to claim 27, wherein array members are comprised of or disposed within a glass.

29. A method according to claim 27, wherein array members are comprised of or disposed within a plastic.

30. A method according to claim 29, the plastic is a polycarbonate polyethylene, polymethylmethacrylate, polystyrene, a copolymer of polystyrene, polysulfone, polyvinylchloride, polyester, polyamide, polyacetal, polyethyleneterephthalate, polytetrafluoroethylene or polyurethane.

31. A method according to claim 30, wherein the plastic is a polycarbonate, polyethylene, polystyrene, a copolymer of polystyrene, polysulfone or polyvinylchloride.

32. A method according to claim 21, wherein the array members are spaced about 1.0 to about 1,000 micrometers apart.

33. A method according to claim 21, wherein the array members have a cross-sectional area of about 1.0 to about 1,000,000 μ

m².

34. A method according to claim 21, wherein the density of array members in the array is about 250 to about 2,500,000 array members per square centimeter of cross sectional surface area of the assay.

35. A method according to claim 21, wherein the density in the array is about 10 to about 100,000 array members per square centimeter of total surface area of the array.

36. A method according to claim 21, wherein there are about 100 to about 2,500,000 aligned array members in the plurality.

37. A method according to claim 21, wherein there are 100 to about 2,500,000 different aligned array members in the plurality.

38. A method according to claim 21, wherein cross-sectioning produces sections about 2.5 to about 2,500,000 micrometers thick.

41. A method according to claim 21, wherein the array comprises analyte binding reagents that hybridize to DNA or RNA having specific nucleotide sequences.

42. A method according to claim 41, wherein the sequence specific binding reagent are polynucleotides, peptide-nucleic acids or polyamides.

43. A method according to claim 42, wherein the sequence specific binding reagents are oligonucleotides.

44. A method according to claim 43, wherein the polypeptide-specific binding reagents are polyclonal antibodies, monoclonal antibodies, a single chain antibody, or an antigen-binding fragment of an antibody.

45. A method according to claim 21, wherein analyte binding reagents are one or more of a nucleic acid, a polynucleotide, a DNA, an RNA, an oligonucleotide, a protein-nucleic acid, an aptamer, a ribozyme, a nucleic acid-binding polyamide, a protein, a peptide, a polypeptide, a glycoprotein, an antibody, an antibody-derived polypeptide, a receptor protein, a fusion protein, a mutein, a lipid, a polysaccharide, a lectin, a ligand, an antigen or a hapten.

46. A method according to claim 21, wherein the array is used to carry out an immunoassay, a hybridization assay, a ligand-binding assay or receptor-binding assay, or a substrate analog affinity assay.

47. A method according to claim 21, wherein binding to the analyte binding reagents is detected using radioactivity, fluorescence, phosphorescence or chemiluminescence.

39. A method for making replica arrays, comprising repeatedly cross-sectioning a plurality of aligned array members to produce sections with at least one surface that exposes array members in the same disposition, thereby replicating the array.

40. A method for making replica arrays for detecting a plurality of analyte, comprising repeatedly cross-sectioning a plurality of aligned analyte binding reagent array members to produce sections with at least one surface that exposes array members in the same disposition, thereby replicating the array.

43-1. A method according to claim 21, wherein the array comprises analyte binding reagents that bind specific polypeptides.